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. 1988 Oct;62(10):3668–3674. doi: 10.1128/jvi.62.10.3668-3674.1988

Inducible expression of herpes simplex virus type 2 glycoprotein gene gG-2 in a mammalian cell line.

H K Su 1, R J Courtney 1
PMCID: PMC253509  PMID: 2843667

Abstract

The gG-2 glycoprotein gene of herpes simplex virus type 2 (HSV-2) was cloned into the mammalian expression vector pMSG under the control of the inducible mouse mammary tumor virus promoter. Transfection of this cloned gG-2 construct into NIH 3T3 cells resulted in the stable expression of gG-2 upon induction with dexamethasone. In addition, the 104,000-molecular-weight (104K) and 72K gG-2 precursors as well as the 34K secreted component were generated in the transformed cells. The synthesis of gG-2 in these transformed cells appeared to follow the same cleavage-processing pathway as gG-2 synthesis during an HSV-2 infection. These results indicate that the processing of gG-2 can occur in the absence of an HSV-2 infection.

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Selected References

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