Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1988 Oct;62(10):3849–3854. doi: 10.1128/jvi.62.10.3849-3854.1988

Investigation of the role of P130gag-fps in transformation: generation and use of a temperature-sensitive mutant P130gag-fps.

G A Weinmaster 1, T Hunter 1
PMCID: PMC253531  PMID: 2843679

Abstract

Changing Glu-1025 to Asp in Fujinami sarcoma virus P130gag-fps made the protein temperature sensitive for transformation and protein-tyrosine kinase activity. Another mutant, Phe-1073 P130gag-fps, lacking the major autophosphorylation site, has an extended latent period for transformation (G. A. Weinmaster, M. J. Zoller, M. Smith, E. Hinze, and T. Pawson, Cell 37:559-568, 1984). By introducing the Asp-1025 lesion into Phe-1073 P130gag-fps, we showed that this mutant protein is required for the maintenance of the transformed phenotype of Phe-1073 P130gag-fps-expressing cells.

Full text

PDF
3849

Images in this article

3851Image
on p.3851
3852Image
on p.3852
3853Image
on p.3853
3853Image
on p.3853

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ariizumi K., Shibuya M. Construction and biological analysis of deletion mutants of Fujinami sarcoma virus: 5'-fps sequence has a role in the transforming activity. J Virol. 1985 Sep;55(3):660–669. doi: 10.1128/jvi.55.3.660-669.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Auersperg N., Pawson T., Worth A., Weinmaster G. Modifications of tumor histology by point mutations in the v-fps oncogene: possible role of extracellular matrix. Cancer Res. 1987 Dec 1;47(23):6341–6348. [PubMed] [Google Scholar]
  3. Chen L. H., Hatada E., Wheatley W., Lee W. H. Single amino acid substitution, from Glu1025 to Asp, of the fps oncogenic protein causes temperature sensitivity in transformation and kinase activity. Virology. 1986 Nov;155(1):106–119. doi: 10.1016/0042-6822(86)90172-8. [DOI] [PubMed] [Google Scholar]
  4. Collett M. S., Belzer S. K., Purchio A. F. Structurally and functionally modified forms of pp60v-src in Rous sarcoma virus-transformed cell lysates. Mol Cell Biol. 1984 Jul;4(7):1213–1220. doi: 10.1128/mcb.4.7.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cooper J. A., Sefton B. M., Hunter T. Detection and quantification of phosphotyrosine in proteins. Methods Enzymol. 1983;99:387–402. doi: 10.1016/0076-6879(83)99075-4. [DOI] [PubMed] [Google Scholar]
  6. Feldman R. A., Hanafusa T., Hanafusa H. Characterization of protein kinase activity associated with the transforming gene product of Fujinami sarcoma virus. Cell. 1980 Dec;22(3):757–765. doi: 10.1016/0092-8674(80)90552-8. [DOI] [PubMed] [Google Scholar]
  7. Hanafusa T., Mathey-Prevot B., Feldman R. A., Hanafusa H. Mutants of Fujinami sarcoma virus which are temperature sensitive for cellular transformation and protein kinase activity. J Virol. 1981 Apr;38(1):347–355. doi: 10.1128/jvi.38.1.347-355.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huang C. C., Hammond C., Bishop J. M. Nucleotide sequence and topography of chicken c-fps. Genesis of a retroviral oncogene encoding a tyrosine-specific protein kinase. J Mol Biol. 1985 Jan 20;181(2):175–186. doi: 10.1016/0022-2836(85)90083-x. [DOI] [PubMed] [Google Scholar]
  9. Hunter T., Sefton B. M. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311–1315. doi: 10.1073/pnas.77.3.1311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kamps M. P., Sefton B. M. Identification of multiple novel polypeptide substrates of the v-src, v-yes, v-fps, v-ros, and v-erb-B oncogenic tyrosine protein kinases utilizing antisera against phosphotyrosine. Oncogene. 1988 Apr;2(4):305–315. [PubMed] [Google Scholar]
  11. Land H., Chen A. C., Morgenstern J. P., Parada L. F., Weinberg R. A. Behavior of myc and ras oncogenes in transformation of rat embryo fibroblasts. Mol Cell Biol. 1986 Jun;6(6):1917–1925. doi: 10.1128/mcb.6.6.1917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lee W. H., Bister K., Moscovici C., Duesberg P. H. Temperature-sensitive mutants of Fujinami sarcoma virus: tumorigenicity and reversible phosphorylation of the transforming p140 protein. J Virol. 1981 Jun;38(3):1064–1076. doi: 10.1128/jvi.38.3.1064-1076.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lee W. H., Bister K., Pawson A., Robins T., Moscovici C., Duesberg P. H. Fujinami sarcoma virus: an avian RNA tumor virus with a unique transforming gene. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2018–2022. doi: 10.1073/pnas.77.4.2018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lee W. H., Liu C. P., Duesberg P. DNA clone of avian Fujinami sarcoma virus with temperature-sensitive transforming function in mammalian cells. J Virol. 1982 Oct;44(1):401–412. doi: 10.1128/jvi.44.1.401-412.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Meckling-Hansen K., Nelson R., Branton P., Pawson T. Enzymatic activation of Fujinami sarcoma virus gag-fps transforming proteins by autophosphorylation at tyrosine. EMBO J. 1987 Mar;6(3):659–666. doi: 10.1002/j.1460-2075.1987.tb04805.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mehta P. P., Bertram J. S., Loewenstein W. R. Growth inhibition of transformed cells correlates with their junctional communication with normal cells. Cell. 1986 Jan 17;44(1):187–196. doi: 10.1016/0092-8674(86)90497-6. [DOI] [PubMed] [Google Scholar]
  17. Morla A. O., Wang J. Y. Protein tyrosine phosphorylation in the cell cycle of BALB/c 3T3 fibroblasts. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8191–8195. doi: 10.1073/pnas.83.21.8191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pawson T., Guyden J., Kung T. H., Radke K., Gilmore T., Martin G. S. A strain of Fujinami sarcoma virus which is temperature-sensitive in protein phosphorylation and cellular transformation. Cell. 1980 Dec;22(3):767–775. doi: 10.1016/0092-8674(80)90553-x. [DOI] [PubMed] [Google Scholar]
  19. Sadowski I., Stone J. C., Pawson T. A noncatalytic domain conserved among cytoplasmic protein-tyrosine kinases modifies the kinase function and transforming activity of Fujinami sarcoma virus P130gag-fps. Mol Cell Biol. 1986 Dec;6(12):4396–4408. doi: 10.1128/mcb.6.12.4396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shibuya M., Hanafusa H. Nucleotide sequence of Fujinami sarcoma virus: evolutionary relationship of its transforming gene with transforming genes of other sarcoma viruses. Cell. 1982 Oct;30(3):787–795. doi: 10.1016/0092-8674(82)90283-5. [DOI] [PubMed] [Google Scholar]
  21. Stone J. C., Atkinson T., Smith M., Pawson T. Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis. Cell. 1984 Jun;37(2):549–558. doi: 10.1016/0092-8674(84)90385-4. [DOI] [PubMed] [Google Scholar]
  22. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weinmaster G. A., Middlemas D. S., Hunter T. A major site of tyrosine phosphorylation within the SH2 domain of Fujinami sarcoma virus P130gag-fps is not required for protein-tyrosine kinase activity or transforming potential. J Virol. 1988 Jun;62(6):2016–2025. doi: 10.1128/jvi.62.6.2016-2025.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weinmaster G., Hinze E., Pawson T. Mapping of multiple phosphorylation sites within the structural and catalytic domains of the Fujinami avian sarcoma virus transforming protein. J Virol. 1983 Apr;46(1):29–41. doi: 10.1128/jvi.46.1.29-41.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weinmaster G., Zoller M. J., Pawson T. A lysine in the ATP-binding site of P130gag-fps is essential for protein-tyrosine kinase activity. EMBO J. 1986 Jan;5(1):69–76. doi: 10.1002/j.1460-2075.1986.tb04179.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weinmaster G., Zoller M. J., Smith M., Hinze E., Pawson T. Mutagenesis of Fujinami sarcoma virus: evidence that tyrosine phosphorylation of P130gag-fps modulates its biological activity. Cell. 1984 Jun;37(2):559–568. doi: 10.1016/0092-8674(84)90386-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES