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. 1988 Dec;62(12):4786–4790. doi: 10.1128/jvi.62.12.4786-4790.1988

Hepatitis B virus particles contain a polypeptide encoded by the largest open reading frame: a putative reverse transcriptase.

D H Mack 1, W Bloch 1, N Nath 1, J J Sninsky 1
PMCID: PMC253600  PMID: 2460642

Abstract

A segment of the largest open reading frame of hepatitis B virus (HBV) was inserted into an open reading frame vector directing the expression in Escherichia coli of a fusion molecule containing 143 HBV-encoded amino acids. The fusion protein was used to generate antiserum which served in immunoblots to identify a polypeptide with a molecular mass of 65 kilodaltons in HBV particles. Because of the small number of molecules in virus particles, unambiguous detection required the development of a highly sensitive immunoblot procedure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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