Abstract
A cDNA containing the complete open reading frame of the Hantaan virus (HTN) M genome segment has been cloned into vaccinia virus. This recombinant virus expresses two glycoproteins which are similar to the HTN structural glycoproteins, G1 and G2, in molecular weight, cleavage pattern, and cellular distribution. Both HTN and recombinant vaccinia virus glycoproteins are exclusively associated with the Golgi apparatus of the cell. Despite this intracellular restriction, mice inoculated with the recombinant vaccinia virus raised neutralizing antibodies against HTN. The specificity of virus neutralization appears to reside in the HTN glycoproteins, since a vaccinia virus recombinant expressing the HTN nucleocapsid protein was unable to elicit a neutralizing antibody response.
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Selected References
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- Chakrabarti S., Brechling K., Moss B. Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques. Mol Cell Biol. 1985 Dec;5(12):3403–3409. doi: 10.1128/mcb.5.12.3403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cheng K. C., Smith G. L., Moss B. Hepatitis B virus large surface protein is not secreted but is immunogenic when selectively expressed by recombinant vaccinia virus. J Virol. 1986 Nov;60(2):337–344. doi: 10.1128/jvi.60.2.337-344.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Childs J. E., Korch G. W., Smith G. A., Terry A. D., Leduc J. W. Geographical distribution and age related prevalence of antibody to Hantaan-like virus in rat populations of Baltimore, Maryland, USA. Am J Trop Med Hyg. 1985 Mar;34(2):385–387. doi: 10.4269/ajtmh.1985.34.385. [DOI] [PubMed] [Google Scholar]
- Elliott L. H., Kiley M. P., McCormick J. B. Hantaan virus: identification of virion proteins. J Gen Virol. 1984 Aug;65(Pt 8):1285–1293. doi: 10.1099/0022-1317-65-8-1285. [DOI] [PubMed] [Google Scholar]
- Eshita Y., Bishop D. H. The complete sequence of the M RNA of snowshoe hare bunyavirus reveals the presence of internal hydrophobic domains in the viral glycoprotein. Virology. 1984 Sep;137(2):227–240. doi: 10.1016/0042-6822(84)90215-0. [DOI] [PubMed] [Google Scholar]
- Ihara T., Smith J., Dalrymple J. M., Bishop D. H. Complete sequences of the glycoproteins and M RNA of Punta Toro phlebovirus compared to those of Rift Valley fever virus. Virology. 1985 Jul 15;144(1):246–259. doi: 10.1016/0042-6822(85)90321-6. [DOI] [PubMed] [Google Scholar]
- Kim G. R., McKee K. T., Jr Pathogenesis of Hantaan virus infection in suckling mice: clinical, virologic, and serologic observations. Am J Trop Med Hyg. 1985 Mar;34(2):388–395. doi: 10.4269/ajtmh.1985.34.388. [DOI] [PubMed] [Google Scholar]
- Lee H. W. Korean hemorrhagic fever. Prog Med Virol. 1982;28:96–113. [PubMed] [Google Scholar]
- Lee P. W., Gibbs C. J., Jr, Gajdusek D. C., Yanagihara R. Serotypic classification of hantaviruses by indirect immunofluorescent antibody and plaque reduction neutralization tests. J Clin Microbiol. 1985 Dec;22(6):940–944. doi: 10.1128/jcm.22.6.940-944.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lees J. F., Pringle C. R., Elliott R. M. Nucleotide sequence of the Bunyamwera virus M RNA segment: conservation of structural features in the Bunyavirus glycoprotein gene product. Virology. 1986 Jan 15;148(1):1–14. doi: 10.1016/0042-6822(86)90398-3. [DOI] [PubMed] [Google Scholar]
- Mackett M., Smith G. L. Vaccinia virus expression vectors. J Gen Virol. 1986 Oct;67(Pt 10):2067–2082. doi: 10.1099/0022-1317-67-10-2067. [DOI] [PubMed] [Google Scholar]
- Mackett M., Yilma T., Rose J. K., Moss B. Vaccinia virus recombinants: expression of VSV genes and protective immunization of mice and cattle. Science. 1985 Jan 25;227(4685):433–435. doi: 10.1126/science.2981435. [DOI] [PubMed] [Google Scholar]
- McKee K. T., Jr, Kim G. R., Green D. E., Peters C. J. Hantaan virus infection in suckling mice: virologic and pathologic correlates. J Med Virol. 1985 Oct;17(2):107–117. doi: 10.1002/jmv.1890170203. [DOI] [PubMed] [Google Scholar]
- Moss B., Smith G. L., Gerin J. L., Purcell R. H. Live recombinant vaccinia virus protects chimpanzees against hepatitis B. Nature. 1984 Sep 6;311(5981):67–69. doi: 10.1038/311067a0. [DOI] [PubMed] [Google Scholar]
- Pereira L., Dondero D., Norrild B., Roizman B. Differential immunologic reactivity and processing of glycoproteins gA and gB of herpes simplex virus types 1 and 2 made in Vero and HEp-2 cells. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5202–5206. doi: 10.1073/pnas.78.8.5202. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Poruchynsky M. S., Tyndall C., Both G. W., Sato F., Bellamy A. R., Atkinson P. H. Deletions into an NH2-terminal hydrophobic domain result in secretion of rotavirus VP7, a resident endoplasmic reticulum membrane glycoprotein. J Cell Biol. 1985 Dec;101(6):2199–2209. doi: 10.1083/jcb.101.6.2199. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rice C. M., Franke C. A., Strauss J. H., Hruby D. E. Expression of Sindbis virus structural proteins via recombinant vaccinia virus: synthesis, processing, and incorporation into mature Sindbis virions. J Virol. 1985 Oct;56(1):227–239. doi: 10.1128/jvi.56.1.227-239.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmaljohn C. S., Dalrymple J. M. Analysis of Hantaan virus RNA: evidence for a new genus of bunyaviridae. Virology. 1983 Dec;131(2):482–491. doi: 10.1016/0042-6822(83)90514-7. [DOI] [PubMed] [Google Scholar]
- Schmaljohn C. S., Hasty S. E., Dalrymple J. M., LeDuc J. W., Lee H. W., von Bonsdorff C. H., Brummer-Korvenkontio M., Vaheri A., Tsai T. F., Regnery H. L. Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome. Science. 1985 Mar 1;227(4690):1041–1044. doi: 10.1126/science.2858126. [DOI] [PubMed] [Google Scholar]
- Schmaljohn C. S., Hasty S. E., Harrison S. A., Dalrymple J. M. Characterization of Hantaan virions, the prototype virus of hemorrhagic fever with renal syndrome. J Infect Dis. 1983 Dec;148(6):1005–1012. doi: 10.1093/infdis/148.6.1005. [DOI] [PubMed] [Google Scholar]
- Schmaljohn C. S., Hasty S. E., Rasmussen L., Dalrymple J. M. Hantaan virus replication: effects of monensin, tunicamycin and endoglycosidases on the structural glycoproteins. J Gen Virol. 1986 Apr;67(Pt 4):707–717. doi: 10.1099/0022-1317-67-4-707. [DOI] [PubMed] [Google Scholar]
- Schmaljohn C. S., Jennings G. B., Hay J., Dalrymple J. M. Coding strategy of the S genome segment of Hantaan virus. Virology. 1986 Dec;155(2):633–643. doi: 10.1016/0042-6822(86)90223-0. [DOI] [PubMed] [Google Scholar]
- Schmaljohn C. S., Schmaljohn A. L., Dalrymple J. M. Hantaan virus M RNA: coding strategy, nucleotide sequence, and gene order. Virology. 1987 Mar;157(1):31–39. doi: 10.1016/0042-6822(87)90310-2. [DOI] [PubMed] [Google Scholar]