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. 1985;63(4):695–703.

Can variola-like viruses be derived from monkeypox virus? An investigation based on DNA mapping*

Joseph J Esposito, James H Nakano, John F Obijeski
PMCID: PMC2536370  PMID: 3002651

Abstract

The results are presented of a special study to determine whether variola-like “whitepox” viruses could arise as white pock variants of monkeypox virus after one or a few mutations. DNA mapping by cross-hybridization of restriction endonuclease DNA fragments was carried out on 18 orthopoxviruses relevant to this study, including variola and monkeypox viruses and white (non-haemorrhagic) pock producers recovered from chorioallantoic membranes infected with red (haemorrhagic) pock-producing monkeypox viruses. The distinctiveness of the DNA maps of true variola and monkeypox viruses indicated that spontaneous production of “whitepox” from monkeypox virus was genetically impossible. These and other observations led to the conclusion that the “whitepox” viruses recovered from monkeypox virus stocks had an exogenous origin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archard L. C., Mackett M., Barnes D. E., Dumbell K. R. The genome structure of cowpox virus white pock variants. J Gen Virol. 1984 May;65(Pt 5):875–886. doi: 10.1099/0022-1317-65-5-875. [DOI] [PubMed] [Google Scholar]
  2. Arita I., Henderson D. A. Monkeypox and whitepox viruses in West and Central Africa. Bull World Health Organ. 1976;53(4):347–353. [PMC free article] [PubMed] [Google Scholar]
  3. Arita I. Virological evidence for the success of the smallpox eradication programme. Nature. 1979 May 24;279(5711):293–298. doi: 10.1038/279293a0. [DOI] [PubMed] [Google Scholar]
  4. DOWNIE A. W., HADDOCK D. W. A variant of cowpox virus. Lancet. 1952 May 24;1(6717):1049–1050. doi: 10.1016/s0140-6736(52)90698-3. [DOI] [PubMed] [Google Scholar]
  5. Dumbell K. R., Archard L. C. Comparison of white pock (h) mutants of monkeypox virus with parental monkeypox and with variola-like viruses isolated from animals. Nature. 1980 Jul 3;286(5768):29–32. doi: 10.1038/286029a0. [DOI] [PubMed] [Google Scholar]
  6. Dumbell K. R., Kapsenberg J. G. Laboratory investigation of two "whitepox" viruses and comparison with two variola strains from southern India. Bull World Health Organ. 1982;60(3):381–387. [PMC free article] [PubMed] [Google Scholar]
  7. Esposito J. J., Cabradilla C. D., Nakano J. H., Obijeski J. F. Intragenomic sequence transposition in monkeypox virus. Virology. 1981 Mar;109(2):231–243. doi: 10.1016/0042-6822(81)90495-5. [DOI] [PubMed] [Google Scholar]
  8. Esposito J. J., Knight J. C. Nucleotide sequence of the thymidine kinase gene region of monkeypox and variola viruses. Virology. 1984 Jun;135(2):561–567. doi: 10.1016/0042-6822(84)90212-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Esposito J. J., Knight J. C. Orthopoxvirus DNA: a comparison of restriction profiles and maps. Virology. 1985 May;143(1):230–251. doi: 10.1016/0042-6822(85)90111-4. [DOI] [PubMed] [Google Scholar]
  10. Esposito J. J., Obijeski J. F., Nakano J. H. Orthopoxvirus DNA: strain differentiation by electrophoresis of restriction endonuclease fragmented virion DNA. Virology. 1978 Aug;89(1):53–66. doi: 10.1016/0042-6822(78)90039-9. [DOI] [PubMed] [Google Scholar]
  11. FENNER F. The biological characters of several strains of vaccinia, cowpox and rabbitpox viruses. Virology. 1958 Jun;5(3):502–529. doi: 10.1016/0042-6822(58)90042-4. [DOI] [PubMed] [Google Scholar]
  12. Fenner F., Sambrook J. F. Conditional lethal mutants of rabbitpox virus. II. Mutants (p) that fail to multiply in PK-2a cells. Virology. 1966 Apr;28(4):600–609. doi: 10.1016/0042-6822(66)90245-5. [DOI] [PubMed] [Google Scholar]
  13. GEMMELL A., FENNER F. Genetic studies with mammalian poxviruses. III. White (u) mutants of rabbitpox virus. Virology. 1960 May;11:219–235. doi: 10.1016/0042-6822(60)90063-5. [DOI] [PubMed] [Google Scholar]
  14. Gibbs A., Fenner F. Methods for comparing sequence data such as restriction endonuclease maps or nucleotide sequences of viral nucleic acid molecules. J Virol Methods. 1984 Dec;9(4):317–324. doi: 10.1016/0166-0934(84)90057-0. [DOI] [PubMed] [Google Scholar]
  15. Gispen R., Brand-Saathof B. "White" poxvirus strains from monkeys. Bull World Health Organ. 1972;46(5):585–592. [PMC free article] [PubMed] [Google Scholar]
  16. Gispen R., Verlinde J. D., Zwart P. Histopathological and virological studies on monkeypox. Arch Gesamte Virusforsch. 1967;21(2):205–216. doi: 10.1007/BF01241445. [DOI] [PubMed] [Google Scholar]
  17. Lake J. R., Cooper P. D. Deletions of the terminal sequences in the genomes of the white pock (u) and host-restricted (p) mutants of rabbitpox virus. J Gen Virol. 1980 May;48(1):135–147. doi: 10.1099/0022-1317-48-1-135. [DOI] [PubMed] [Google Scholar]
  18. Mackett M., Archard L. C. Conservation and variation in Orthopoxvirus genome structure. J Gen Virol. 1979 Dec;45(3):683–701. doi: 10.1099/0022-1317-45-3-683. [DOI] [PubMed] [Google Scholar]
  19. Marennikova S. S., Seluhina E. M., Mal'ceva N. N., Ladnyj I. D. Poxviruses isolated from clinically ill and asymptomatically infected monkeys and a chimpanzee. Bull World Health Organ. 1972;46(5):613–620. [PMC free article] [PubMed] [Google Scholar]
  20. Marennikova S. S., Shelukhina E. M., Maltseva N. N., Matsevich G. R. Monkeypox virus as a source of whitepox viruses. Intervirology. 1979;11(6):333–340. doi: 10.1159/000149055. [DOI] [PubMed] [Google Scholar]
  21. Marennikova S. S., Shelukhina E. M., Maltseva N. N. Monkeypox virus and whitepox viruses. Acta Virol. 1978 Nov;22(6):512–512. [PubMed] [Google Scholar]
  22. Marennikova S. S., Shelukhina E. M., Shenkman L. S. "White-wild" (variola-like) poxvirus strains from rodents in Equatorial Africa. Acta Virol. 1976 Feb;20(1):80–82. [PubMed] [Google Scholar]
  23. Marennikova S. S., Shelukhina E. M. Whitepox virus isolated from hamsters inoculated with monkeypox virus. Nature. 1978 Nov 16;276(5685):291–292. doi: 10.1038/276291a0. [DOI] [PubMed] [Google Scholar]
  24. Moyer R. W., Brown G. D., Graves R. L. The white pock mutants of rabbit poxvirus. II. The early white pock (mu) host range (hr) mutants of rabbit poxvirus uncouple transcription and translation in nonpermissive cells. Virology. 1980 Oct 30;106(2):234–249. doi: 10.1016/0042-6822(80)90247-0. [DOI] [PubMed] [Google Scholar]
  25. Moyer R. W., Rothe C. T. The white pock mutants of rabbit poxvirus. I. Spontaneous host range mutants contain deletions. Virology. 1980 Apr 15;102(1):119–132. doi: 10.1016/0042-6822(80)90075-6. [DOI] [PubMed] [Google Scholar]
  26. VAN TONGEREN H. A. E. Spontaneous mutation of cowpox-virus by means of egg-passage. Arch Gesamte Virusforsch. 1952;5(1):35–52. doi: 10.1007/BF01245138. [DOI] [PubMed] [Google Scholar]

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