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. 1988 Mar;62(3):875–881. doi: 10.1128/jvi.62.3.875-881.1988

Identification and characterization of three distinct families of glycoprotein complexes in the envelopes of human cytomegalovirus.

D R Gretch 1, B Kari 1, L Rasmussen 1, R C Gehrz 1, M F Stinski 1
PMCID: PMC253645  PMID: 2828682

Abstract

Several disulfide-linked glycoprotein complexes were identified in the envelope of human cytomegalovirus (HCMV). These glycoprotein complexes were fractionated by rate-zonal centrifugation in sucrose density gradients in the presence of detergents. Fractionated glycoproteins and complexes were immunoprecipitated with three different monoclonal antibodies specific for HCMV glycoproteins and a rabbit polyclonal antiserum prepared against detergent-extracted virion and dense-body envelope glycoproteins. Three distinct families of disulfide-linked glycoprotein complexes were observed and designated glycoprotein complex gcI, gcII, and gcIII. The gcI family, recognized by monoclonal antibody 41C2 under nonreducing conditions, consisted of three complexes with approximate molecular masses of 250 to 300, 190, and 160 kilodaltons (kDa). These complexes consistently sediment more rapidly than other HCMV glycoproteins or complexes in sucrose density gradients. Upon reduction of the gcI family, two size classes of glycoproteins with average molecular masses of 93 to 130 and 55 kDa were observed. The gcII family was recognized by monoclonal antibody 9E10. Under nonreducing conditions, as many as six electrophoretic forms were observed for gcII. When reduced, the major component of the gcII family was a heterogeneous glycoprotein designated gp47-52. The gcIII family was recognized by monoclonal antibody 1G6. It consisted of a complex of approximately 240 kDa without reduction of disulfide bonds. When reduced, two glycoprotein size classes with average molecular masses of 145 and 86 kDa were observed. Polyclonal antiserum R-7 reacted strongly with the gcI and gcIII families, but weakly with the gcII family.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Britt W. J., Auger D. Identification of a 65 000 dalton virion envelope protein of human cytomegalovirus. Virus Res. 1985 Dec;4(1):31–36. doi: 10.1016/0168-1702(85)90018-8. [DOI] [PubMed] [Google Scholar]
  2. Britt W. J., Auger D. Synthesis and processing of the envelope gp55-116 complex of human cytomegalovirus. J Virol. 1986 Apr;58(1):185–191. doi: 10.1128/jvi.58.1.185-191.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Britt W. J. Neutralizing antibodies detect a disulfide-linked glycoprotein complex within the envelope of human cytomegalovirus. Virology. 1984 Jun;135(2):369–378. doi: 10.1016/0042-6822(84)90193-4. [DOI] [PubMed] [Google Scholar]
  4. Cranage M. P., Kouzarides T., Bankier A. T., Satchwell S., Weston K., Tomlinson P., Barrell B., Hart H., Bell S. E., Minson A. C. Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus. EMBO J. 1986 Nov;5(11):3057–3063. doi: 10.1002/j.1460-2075.1986.tb04606.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farrar G. H., Greenaway P. J. Characterization of glycoprotein complexes present in human cytomegalovirus envelopes. J Gen Virol. 1986 Jul;67(Pt 7):1469–1473. doi: 10.1099/0022-1317-67-7-1469. [DOI] [PubMed] [Google Scholar]
  6. Farrar G. H., Oram J. D. Characterization of the human cytomegalovirus envelope glycoproteins. J Gen Virol. 1984 Nov;65(Pt 11):1991–2001. doi: 10.1099/0022-1317-65-11-1991. [DOI] [PubMed] [Google Scholar]
  7. Gretch D. R., Suter M., Stinski M. F. The use of biotinylated monoclonal antibodies and streptavidin affinity chromatography to isolate herpesvirus hydrophobic proteins or glycoproteins. Anal Biochem. 1987 May 15;163(1):270–277. doi: 10.1016/0003-2697(87)90123-0. [DOI] [PubMed] [Google Scholar]
  8. Gönczöl E., Hudecz F., Ianacone J., Dietzschold B., Starr S., Plotkin S. A. Immune responses to isolated human cytomegalovirus envelope proteins. J Virol. 1986 May;58(2):661–664. doi: 10.1128/jvi.58.2.661-664.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kari B., Lussenhop N., Goertz R., Wabuke-Bunoti M., Radeke R., Gehrz R. Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes. J Virol. 1986 Nov;60(2):345–352. doi: 10.1128/jvi.60.2.345-352.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kim K. S., Sapienza V. J., Chen C. M., Wisniewski K. Production and characterization of monoclonal antibodies specific for a glycosylated polypeptide of human cytomegalovirus. J Clin Microbiol. 1983 Aug;18(2):331–343. doi: 10.1128/jcm.18.2.331-343.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Law K. M., Wilton-Smith P., Farrar G. H. A murine monoclonal antibody recognising a single glycoprotein within a human cytomegalovirus virion envelope glycoprotein complex. J Med Virol. 1985 Nov;17(3):255–266. doi: 10.1002/jmv.1890170307. [DOI] [PubMed] [Google Scholar]
  12. Mach M., Utz U., Fleckenstein B. Mapping of the major glycoprotein gene of human cytomegalovirus. J Gen Virol. 1986 Jul;67(Pt 7):1461–1467. doi: 10.1099/0022-1317-67-7-1461. [DOI] [PubMed] [Google Scholar]
  13. Pereira L., Hoffman M., Cremer N. Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus-infected cell extracts by human sera. Infect Immun. 1982 Jun;36(3):933–942. doi: 10.1128/iai.36.3.933-942.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pereira L., Hoffman M., Gallo D., Cremer N. Monoclonal antibodies to human cytomegalovirus: three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants. Infect Immun. 1982 Jun;36(3):924–932. doi: 10.1128/iai.36.3.924-932.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pereira L., Hoffman M., Tatsuno M., Dondero D. Polymorphism of human cytomegalovirus glycoproteins characterized by monoclonal antibodies. Virology. 1984 Nov;139(1):73–86. doi: 10.1016/0042-6822(84)90331-3. [DOI] [PubMed] [Google Scholar]
  16. Rasmussen L. E., Nelson R. M., Kelsall D. C., Merigan T. C. Murine monoclonal antibody to a single protein neutralizes the infectivity of human cytomegalovirus. Proc Natl Acad Sci U S A. 1984 Feb;81(3):876–880. doi: 10.1073/pnas.81.3.876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rasmussen L., Mullenax J., Nelson M., Merigan T. C. Human cytomegalovirus polypeptides stimulate neutralizing antibody in vivo. Virology. 1985 Aug;145(1):186–190. doi: 10.1016/0042-6822(85)90215-6. [DOI] [PubMed] [Google Scholar]
  18. Rasmussen L., Mullenax J., Nelson R., Merigan T. C. Viral polypeptides detected by a complement-dependent neutralizing murine monoclonal antibody to human cytomegalovirus. J Virol. 1985 Aug;55(2):274–280. doi: 10.1128/jvi.55.2.274-280.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schmitz H., Müller-Lantzsch N., Peteler G. Human immune response to proteins of cytomegalovirus. Intervirology. 1980;13(3):154–161. doi: 10.1159/000149120. [DOI] [PubMed] [Google Scholar]
  20. Stinski M. F. Human cytomegalovirus: glycoproteins associated with virions and dense bodies. J Virol. 1976 Aug;19(2):594–609. doi: 10.1128/jvi.19.2.594-609.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stinski M. F., Mocarski E. S., Thomsen D. R., Urbanowski M. L. Membrane glycoproteins and antigens induced by human cytomegalovirus. J Gen Virol. 1979 Apr;43(1):119–129. doi: 10.1099/0022-1317-43-1-119. [DOI] [PubMed] [Google Scholar]
  22. Stinski M. F. Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus-induced polypeptides. J Virol. 1978 Jun;26(3):686–701. doi: 10.1128/jvi.26.3.686-701.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stinski M. F. Synthesis of proteins and glycoproteins in cells infected with human cytomegalovirus. J Virol. 1977 Sep;23(3):751–767. doi: 10.1128/jvi.23.3.751-767.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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