Figure 2.

Dot blot assay of representative CHO cell-derived IgG1 PA-specific human monoclonal antibodies. PA83 and PA63 were purchased from List Biological Laboratories. D4-GFP and PA20-GFP were expressed and purified from E. coli as described in the materials and methods section. All antigens were spotted at 1 µg per spot using a 96 well manifold. Blots were cut into strips and probed with individual PA-specific monoclonal antibodies.