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. 1988 Aug;62(8):2736–2744. doi: 10.1128/jvi.62.8.2736-2744.1988

Generation and characterization of a temperature-sensitive mutation in the NS-1 gene of the autonomous parvovirus minute virus of mice.

G E Tullis 1, L Labieniec-Pintel 1, K E Clemens 1, D Pintel 1
PMCID: PMC253707  PMID: 2969054

Abstract

In-phase single-codon insertion mutations were constructed in the open reading frames of the NS-1 and NS-2 genes of the autonomous parvovirus minute virus of mice. A viral mutant containing an isoleucine insertion exclusively within NS-1 between residues 229 and 230 was isolated that produced approximately 3 orders of magnitude fewer plaques at 39 degrees C than at 32 degrees C. Preliminary characterization of the mutant demonstrated that the NS-1 gene product is independently required for both genome amplification and the regulation of the temporal expression between the two viral transcription units during lytic infection.

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Selected References

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