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. 1988 Aug;62(8):2884–2889. doi: 10.1128/jvi.62.8.2884-2889.1988

The gene encoding the nonstructural protein of B19 (human) parvovirus may be lethal in transfected cells.

K Ozawa 1, J Ayub 1, S Kajigaya 1, T Shimada 1, N Young 1
PMCID: PMC253725  PMID: 2969055

Abstract

The B19 parvovirus is a cause of bone marrow failure in humans. B19 is toxic to erythroid progenitor cells in vitro. Viral products possibly responsible for toxicity were explored by transfection of cloned B19 genome into HeLa cells. The nonstructural (NS) protein was detected in cells 30 h after transfection. Plasmids containing the B19 genome were transfected with selectable marker genes in stable transformation assays. Plasmids that contained the left side of the B19 genome, which encodes the NS protein of the virus, inhibited antibiotic-resistant colony formation. Transformation occurred when NS protein expression was blocked by mutation. Suppression of transformation by NS protein was not tissue specific, suggesting a role for NS protein in toxicity for nonpermissive cells without parvovirus replication or virion accumulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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