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. 1959;20(6):1133–1150.

Consistency in potency assay of tetanus toxoid in mice

H Cohen, J D van Ramshorst, A Tasman
PMCID: PMC2537883  PMID: 13810954

Abstract

The use of mice for the assay of tetanus toxoids would offer considerable advantages over the use of guinea-pigs, but mice cannot readily be immunized with the fluid tetanus toxoid at present designated as the International Standard. This study shows, however, that the mouse is a very suitable laboratory animal for the comparison of adsorbed tetanus toxoids, and that an AlPO4-adsorbed vaccine, which is stable at 4°C, is a satisfactory reference preparation. The log-dose-response lines of toxoids adsorbed on different quantities of AlPO4 and on various quantities of another adsorbent ran parallel to those of the reference vaccine. The 95% confidence limits for the potencies of tetanus vaccines, diphtheria-tetanus vaccines, and diphtheria-pertussis-tetanus vaccines, determined by assay against the reference vaccine in mice, showed a high degree of reproducibility of the results.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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