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. 1986 Oct;60(1):90–96. doi: 10.1128/jvi.60.1.90-96.1986

Conserved epitopes on the hemagglutinin-neuraminidase proteins of human and bovine parainfluenza type 3 viruses: nucleotide sequence analysis of variants selected with monoclonal antibodies.

K J Coelingh, C C Winter, B R Murphy, J M Rice, P C Kimball, R A Olmsted, P L Collins
PMCID: PMC253905  PMID: 2427750

Abstract

We have previously identified 11 epitopes located in two topologically nonoverlapping antigenic sites (A and B) and a third bridging site (C) on the human type 3 parainfluenza virus (PIV3) hemagglutinin-neuraminidase (HN) glycoprotein by using monoclonal antibodies (MAbs) which inhibit hemagglutination and virus infectivity (K. L. Coelingh, C. C. Winter, and B. R. Murphy, Virology 143:569-582, 1985). We have identified three additional antigenic sites (D, E, and F) on the HN molecule by competitive-binding assays of anti-HN MAbs which have no known biological activity. Epitopes in sites A, D, and F are conserved on the bovine PIV3 HN glycoprotein and also among a wide range of human isolates. The dideoxy method was used to identify nucleotide substitutions in the HN genes of antigenic variants selected with neutralizing MAbs representing epitopes in site A which are shared by human and bovine PIV3. The deduced amino acid substitutions in the variants were located in separate hydrophilic stretches of HN residues which are conserved in the primary structures of the HN proteins of both human and bovine PIV3 strains.

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Selected References

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  1. ABINANTI F. R., CHANOCK R. M., COOK M. K., WONG D., WARFIELD M. Relationship of human and bovine strains of myxovirus para-influenza 3. Proc Soc Exp Biol Med. 1961 Mar;106:466–469. doi: 10.3181/00379727-106-26371. [DOI] [PubMed] [Google Scholar]
  2. ABINANTI F. R., HUEBNER R. J. The serological relationships of strains of parainfluenza 3 virus isolated from humans and cattle with respiratory disease. Virology. 1959 Jul;8(3):391–394. doi: 10.1016/0042-6822(59)90041-8. [DOI] [PubMed] [Google Scholar]
  3. Air G. M., Els M. C., Brown L. E., Laver W. G., Webster R. G. Location of antigenic sites on the three-dimensional structure of the influenza N2 virus neuraminidase. Virology. 1985 Sep;145(2):237–248. doi: 10.1016/0042-6822(85)90157-6. [DOI] [PubMed] [Google Scholar]
  4. Air G. M. Nucleotide sequence coding for the "signal peptide" and N terminus of the hemagglutinin from an asian (H2N2) strain of influenza virus. Virology. 1979 Sep;97(2):468–472. doi: 10.1016/0042-6822(79)90358-1. [DOI] [PubMed] [Google Scholar]
  5. Aymard-Henry M., Coleman M. T., Dowdle W. R., Laver W. G., Schild G. C., Webster R. G. Influenzavirus neuraminidase and neuraminidase-inhibition test procedures. Bull World Health Organ. 1973;48(2):199–202. [PMC free article] [PubMed] [Google Scholar]
  6. Bean W. J., Jr, Sriram G., Webster R. G. Electrophoretic analysis of iodine-labeled influenza virus RNA segments. Anal Biochem. 1980 Feb;102(1):228–232. doi: 10.1016/0003-2697(80)90343-7. [DOI] [PubMed] [Google Scholar]
  7. Berton M. T., Naeve C. W., Webster R. G. Antigenic structure of the influenza B virus hemagglutinin: nucleotide sequence analysis of antigenic variants selected with monoclonal antibodies. J Virol. 1984 Dec;52(3):919–927. doi: 10.1128/jvi.52.3.919-927.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Blumberg B., Giorgi C., Roux L., Raju R., Dowling P., Chollet A., Kolakofsky D. Sequence determination of the Sendai virus HN gene and its comparison to the influenza virus glycoproteins. Cell. 1985 May;41(1):269–278. doi: 10.1016/0092-8674(85)90080-7. [DOI] [PubMed] [Google Scholar]
  10. CHANOCK R. M., PARROTT R. H., COOK K., ANDREWS B. E., BELL J. A., REICHELDERFER T., KAPIKIAN A. Z., MASTROTA F. M., HUEBNER R. J. Newly recognized myxoviruses from children with respiratory disease. N Engl J Med. 1958 Jan 30;258(5):207–213. doi: 10.1056/NEJM195801302580502. [DOI] [PubMed] [Google Scholar]
  11. Caton A. J., Brownlee G. G., Yewdell J. W., Gerhard W. The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype). Cell. 1982 Dec;31(2 Pt 1):417–427. doi: 10.1016/0092-8674(82)90135-0. [DOI] [PubMed] [Google Scholar]
  12. Dubin D. T., Montoya J., Timko K. D., Attardi G. Sequence analysis and precise mapping of the 3' ends of HeLa cell mitochondrial ribosomal RNAs. J Mol Biol. 1982 May 5;157(1):1–19. doi: 10.1016/0022-2836(82)90510-1. [DOI] [PubMed] [Google Scholar]
  13. Elango N., Coligan J. E., Jambou R. C., Venkatesan S. Human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mRNA and limited amino acid sequence of the purified protein. J Virol. 1986 Feb;57(2):481–489. doi: 10.1128/jvi.57.2.481-489.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Guskey L. E., Bergtrom G. High yield growth and purification of human parainfluenza type 3 virus and initial analysis of viral structural proteins. J Gen Virol. 1981 May;54(Pt 1):115–123. doi: 10.1099/0022-1317-54-1-115. [DOI] [PubMed] [Google Scholar]
  15. Jambou R. C., Elango N., Venkatesan S. Proteins associated with human parainfluenza virus type 3. J Virol. 1985 Oct;56(1):298–302. doi: 10.1128/jvi.56.1.298-302.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. REISINGER R. C., HEDDLESTON K. L., MANTHEI C. A. A myxovirus (SF-4) associated with shipping fever of cattle. J Am Vet Med Assoc. 1959 Aug 1;135(3):147–152. [PubMed] [Google Scholar]
  17. Seif I., Coulon P., Rollin P. E., Flamand A. Rabies virulence: effect on pathogenicity and sequence characterization of rabies virus mutations affecting antigenic site III of the glycoprotein. J Virol. 1985 Mar;53(3):926–934. doi: 10.1128/jvi.53.3.926-934.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shibuta H., Kanda T., Adachi A., Yogo Y. Characterization of bovine parainfluenza virus type 3. Microbiol Immunol. 1979;23(7):617–628. doi: 10.1111/j.1348-0421.1979.tb00502.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Storey D. G., Dimock K., Kang C. Y. Structural characterization of virion proteins and genomic RNA of human parainfluenza virus 3. J Virol. 1984 Dec;52(3):761–766. doi: 10.1128/jvi.52.3.761-766.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sánchez A., Banerjee A. K. Studies on human parainfluenza virus 3: characterization of the structural proteins and in vitro synthesized proteins coded by mRNAs isolated from infected cells. Virology. 1985 May;143(1):45–54. doi: 10.1016/0042-6822(85)90095-9. [DOI] [PubMed] [Google Scholar]
  21. Webster R. G., Laver W. G. Determination of the number of nonoverlapping antigenic areas on Hong Kong (H3N2) influenza virus hemagglutinin with monoclonal antibodies and the selection of variants with potential epidemiological significance. Virology. 1980 Jul 15;104(1):139–148. doi: 10.1016/0042-6822(80)90372-4. [DOI] [PubMed] [Google Scholar]
  22. Wilbur W. J., Lipman D. J. Rapid similarity searches of nucleic acid and protein data banks. Proc Natl Acad Sci U S A. 1983 Feb;80(3):726–730. doi: 10.1073/pnas.80.3.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van Wyke Coelingh K. L., Winter C., Murphy B. R. Antigenic variation in the hemagglutinin-neuraminidase protein of human parainfluenza type 3 virus. Virology. 1985 Jun;143(2):569–582. doi: 10.1016/0042-6822(85)90395-2. [DOI] [PubMed] [Google Scholar]

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