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. 1986 Oct;60(1):224–229. doi: 10.1128/jvi.60.1.224-229.1986

Complete Sequence and Enhancer Function of the Homologous DNA Regions of Autographa californica Nuclear Polyhedrosis Virus

Linda A Guarino 1,*, Mark A Gonzalez 1, Max D Summers 1
PMCID: PMC253920  PMID: 16789259

Abstract

The nucleotide sequence of the five regions of homologous DNA in the genome of Autographa californica nuclear polyhedrosis virus DNA was determined. The homology of repeated sequences within a region was 65 to 87%, and the consensus sequences for each region were 88% homologous to each other. Sequences proximal to the EcoRI sites were most conserved, while the distal sequences were least conserved. The EcoRI sites formed the core of a 28-base-pair imperfect inverted repeat. All homologous regions functioned as enhancers in a transient expression assay. A single EcoRI minifragment located between EcoRI-Q and -L enhanced the expression of 39CAT as efficiently as the regions containing numerous EcoRI repeats did.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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