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. 1987 Feb;61(2):395–404. doi: 10.1128/jvi.61.2.395-404.1987

A 14,000-Mr envelope protein of vaccinia virus is involved in cell fusion and forms covalently linked trimers.

J F Rodriguez, E Paez, M Esteban
PMCID: PMC253962  PMID: 3806791

Abstract

A monoclonal antibody, MAbC3, that reacts with a 14,000-molecular-weight envelope protein (14K protein) of vaccinia virus completely inhibited virus-induced cell fusion during infection. Immunoblot and immunofluorescence studies revealed that the 14K protein was synthesized at about 6 to 7 h postinfection and transported from the cytoplasm to the cell surface. Synthesis and transport of the 14K protein during infection occurred in the presence of rifampin, an inhibitor of virus maturation. One- and two-dimensional gel electrophoretic analyses demonstrated that the 14K protein forms largely trimers (42K) that are covalently linked by disulfide bonds. The facts that MAbC3 prevents virus uncoating and blocks virus-induced cell fusion but does not prevent virus attachment to cells and the 14K envelope protein forms trimers all suggest that this protein plays major role in virus penetration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong J. A., Metz D. H., Young M. R. The mode of entry of vaccinia virus into L cells. J Gen Virol. 1973 Dec;21(3):533–537. doi: 10.1099/0022-1317-21-3-533. [DOI] [PubMed] [Google Scholar]
  2. Carrasco L., Bravo R. Specific proteins synthesized during the viral lytic cycle in vaccinia virus-infected HeLa cells: analysis by high-resolution, two-dimensional gel electrophoresis. J Virol. 1986 May;58(2):569–577. doi: 10.1128/jvi.58.2.569-577.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carrasco L., Esteban M. Modification of membrane permeability in vaccinia virus-infected cells. Virology. 1982 Feb;117(1):62–69. doi: 10.1016/0042-6822(82)90507-4. [DOI] [PubMed] [Google Scholar]
  4. Eppstein D. A., Marsh Y. V., Schreiber A. B., Newman S. R., Todaro G. J., Nestor J. J., Jr Epidermal growth factor receptor occupancy inhibits vaccinia virus infection. Nature. 1985 Dec 19;318(6047):663–665. doi: 10.1038/318663a0. [DOI] [PubMed] [Google Scholar]
  5. Essani K., Dales S. Biogenesis of vaccinia: evidence for more than 100 polypeptides in the virion. Virology. 1979 Jun;95(2):385–394. doi: 10.1016/0042-6822(79)90493-8. [DOI] [PubMed] [Google Scholar]
  6. Esteban M. Defective vaccinia virus particles in interferon-treated infected cells. Virology. 1984 Feb;133(1):220–227. doi: 10.1016/0042-6822(84)90443-4. [DOI] [PubMed] [Google Scholar]
  7. Grimley P. M., Rosenblum E. N., Mims S. J., Moss B. Interruption by Rifampin of an early stage in vaccinia virus morphogenesis: accumulation of membranes which are precursors of virus envelopes. J Virol. 1970 Oct;6(4):519–533. doi: 10.1128/jvi.6.4.519-533.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ichihashi Y., Oie M. Adsorption and penetration of the trypsinized vaccinia virion. Virology. 1980 Feb;101(1):50–60. doi: 10.1016/0042-6822(80)90482-1. [DOI] [PubMed] [Google Scholar]
  9. Ichihashi Y., Oie M. Proteolytic activation of vaccinia virus for the penetration phase of infection. Virology. 1982 Jan 15;116(1):297–305. doi: 10.1016/0042-6822(82)90421-4. [DOI] [PubMed] [Google Scholar]
  10. JOKLIK W. K. The purification fo four strains of poxvirus. Virology. 1962 Sep;18:9–18. doi: 10.1016/0042-6822(62)90172-1. [DOI] [PubMed] [Google Scholar]
  11. Lane J. M., Ruben F. L., Neff J. M., Millar J. D. Complications of smallpox vaccination, 1968. N Engl J Med. 1969 Nov 27;281(22):1201–1208. doi: 10.1056/NEJM196911272812201. [DOI] [PubMed] [Google Scholar]
  12. Mackett M., Smith G. L., Moss B. Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415–7419. doi: 10.1073/pnas.79.23.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mallon V. R., Domber E. A., Holowczak J. A. Vaccinia virus proteins on the plasma membranes of infected cells. II. Expression of viral antigens and killing of infected cells by vaccinia virus-specific cytotoxic T cells. Virology. 1985 Aug;145(1):1–23. doi: 10.1016/0042-6822(85)90197-7. [DOI] [PubMed] [Google Scholar]
  14. Mallon V., Holowczak J. A. Vaccinia virus antigens on the plasma membrane of infected cells. I. Viral antigens transferred from infecting virus particles and synthesized after infection. Virology. 1985 Mar;141(2):201–220. doi: 10.1016/0042-6822(85)90252-1. [DOI] [PubMed] [Google Scholar]
  15. Nagaya A., Pogo B. G., Dales S. Biogenesis of vaccinia: separation of early stages from maturation by means of rifampicin. Virology. 1970 Apr;40(4):1039–1051. doi: 10.1016/0042-6822(70)90150-9. [DOI] [PubMed] [Google Scholar]
  16. Paez E., Dallo S., Esteban M. Generation of a dominant 8-MDa deletion at the left terminus of vaccinia virus DNA. Proc Natl Acad Sci U S A. 1985 May;82(10):3365–3369. doi: 10.1073/pnas.82.10.3365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Paez E., Esteban M. Interferon prevents the generation of spontaneous deletions at the left terminus of vaccinia virus DNA. J Virol. 1985 Oct;56(1):75–84. doi: 10.1128/jvi.56.1.75-84.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Panicali D., Paoletti E. Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4927–4931. doi: 10.1073/pnas.79.16.4927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Payne L. G. Identification of the vaccinia hemagglutinin polypeptide from a cell system yielding large amounts of extracellular enveloped virus. J Virol. 1979 Jul;31(1):147–155. doi: 10.1128/jvi.31.1.147-155.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rodriguez J. F., Janeczko R., Esteban M. Isolation and characterization of neutralizing monoclonal antibodies to vaccinia virus. J Virol. 1985 Nov;56(2):482–488. doi: 10.1128/jvi.56.2.482-488.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shida H., Dales S. Biogenesis of vaccinia: carbohydrate of the hemagglutinin molecules. Virology. 1981 May;111(1):56–72. doi: 10.1016/0042-6822(81)90653-x. [DOI] [PubMed] [Google Scholar]
  22. Stern W., Dales S. Biogenesis of vaccinia: isolation and characterization of a surface component that elicits antibody suppressing infectivity and cell-cell fusion. Virology. 1976 Nov;75(1):232–241. doi: 10.1016/0042-6822(76)90022-2. [DOI] [PubMed] [Google Scholar]
  23. Stroobant P., Rice A. P., Gullick W. J., Cheng D. J., Kerr I. M., Waterfield M. D. Purification and characterization of vaccinia virus growth factor. Cell. 1985 Aug;42(1):383–393. doi: 10.1016/s0092-8674(85)80133-1. [DOI] [PubMed] [Google Scholar]

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