Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1987 Mar;61(3):755–763. doi: 10.1128/jvi.61.3.755-763.1987

Derivation and characterization of POJ cells, transformed human fetal glial cells that retain their permissivity for JC virus.

C Mandl, D L Walker, R J Frisque
PMCID: PMC254017  PMID: 3027403

Abstract

The study of the medically important polyomavirus JC virus is limited to only a few laboratories, primarily because the permissive cell system most often used, primary human fetal glial cells, is difficult to obtain and propagate. We have introduced mutations at the origin of DNA replication of JC virus and transformed glial cells with the replication-defective genomes. Although normal glial cell cultures rapidly lose their permissivity for the virus after subculture, the transformed cells (designated POJ) had a greatly expanded life span and remained permissive for JC virus even after 30 passages in vitro. POJ cells constitutively express a functional T protein that complements the replication defect of lethal early-region mutations in JC virus. We expect that these cells will greatly facilitate the study of this human virus.

Full text

PDF
755

Images in this article

758Image
on p.758
759Image
on p.759
760Image
on p.760
761Image
on p.761

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckmann A. M., Shah K. V., Padgett B. L. Propagation and primary isolation of papovavirus JC in epithelial cells derived from human urine. Infect Immun. 1982 Nov;38(2):774–777. doi: 10.1128/iai.38.2.774-777.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Botchan M., Topp W., Sambrook J. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell. 1976 Oct;9(2):269–287. doi: 10.1016/0092-8674(76)90118-5. [DOI] [PubMed] [Google Scholar]
  3. Chuke W. F., Walker D. L., Peitzman L. B., Frisque R. J. Construction and characterization of hybrid polyomavirus genomes. J Virol. 1986 Dec;60(3):960–971. doi: 10.1128/jvi.60.3.960-971.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeLucia A. L., Lewton B. A., Tjian R., Tegtmeyer P. Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication. J Virol. 1983 Apr;46(1):143–150. doi: 10.1128/jvi.46.1.143-150.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deb S., DeLucia A. L., Baur C. P., Koff A., Tegtmeyer P. Domain structure of the simian virus 40 core origin of replication. Mol Cell Biol. 1986 May;6(5):1663–1670. doi: 10.1128/mcb.6.5.1663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frisque R. J., Bream G. L., Cannella M. T. Human polyomavirus JC virus genome. J Virol. 1984 Aug;51(2):458–469. doi: 10.1128/jvi.51.2.458-469.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Frisque R. J. Nucleotide sequence of the region encompassing the JC virus origin of DNA replication. J Virol. 1983 Apr;46(1):170–176. doi: 10.1128/jvi.46.1.170-176.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frisque R. J. Regulatory sequences and virus-cell interactions of JC virus. Prog Clin Biol Res. 1983;105:41–59. [PubMed] [Google Scholar]
  9. Frisque R. J., Rifkin D. B., Walker D. L. Transformation of primary hamster brain cells with JC virus and its DNA. J Virol. 1980 Jul;35(1):265–269. doi: 10.1128/jvi.35.1.265-269.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gluzman Y., Frisque R. J., Sambrook J. Origin-defective mutants of SV40. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):293–300. doi: 10.1101/sqb.1980.044.01.033. [DOI] [PubMed] [Google Scholar]
  11. Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell. 1981 Jan;23(1):175–182. doi: 10.1016/0092-8674(81)90282-8. [DOI] [PubMed] [Google Scholar]
  12. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  13. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  14. Howley P. M., Rentier-Delrue F., Heilman C. A., Law M. F., Chowdhury K., Israel M. A., Takemoto K. K. Cloned human polyomavirus JC DNA can transform human amnion cells. J Virol. 1980 Dec;36(3):878–882. doi: 10.1128/jvi.36.3.878-882.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jasin M., de Villiers J., Weber F., Schaffner W. High frequency of homologous recombination in mammalian cells between endogenous and introduced SV40 genomes. Cell. 1985 Dec;43(3 Pt 2):695–703. doi: 10.1016/0092-8674(85)90242-9. [DOI] [PubMed] [Google Scholar]
  16. Ketner G., Kelly T. J., Jr Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1102–1106. doi: 10.1073/pnas.73.4.1102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lanford R. E., Butel J. S. Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen. Cell. 1984 Jul;37(3):801–813. doi: 10.1016/0092-8674(84)90415-x. [DOI] [PubMed] [Google Scholar]
  18. Li J. J., Kelly T. J. Simian virus 40 DNA replication in vitro: specificity of initiation and evidence for bidirectional replication. Mol Cell Biol. 1985 Jun;5(6):1238–1246. doi: 10.1128/mcb.5.6.1238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Li J. J., Peden K. W., Dixon R. A., Kelly T. Functional organization of the simian virus 40 origin of DNA replication. Mol Cell Biol. 1986 Apr;6(4):1117–1128. doi: 10.1128/mcb.6.4.1117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Major E. O., Miller A. E., Mourrain P., Traub R. G., de Widt E., Sever J. Establishment of a line of human fetal glial cells that supports JC virus multiplication. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1257–1261. doi: 10.1073/pnas.82.4.1257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mandl C. W., Frisque R. J. Characterization of cells transformed by the human polyomavirus JC virus. J Gen Virol. 1986 Aug;67(Pt 8):1733–1739. doi: 10.1099/0022-1317-67-8-1733. [DOI] [PubMed] [Google Scholar]
  22. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  23. Miller N. R., London W., Padgett B. L., Walker D. L., Wallen W. C. The detection of JC viral genome in owl monkey tumors. Prog Clin Biol Res. 1983;105:271–288. [PubMed] [Google Scholar]
  24. Miyamura T., Jikuya H., Soeda E., Yoshiike K. Genomic structure of human polyoma virus JC: nucleotide sequence of the region containing replication origin and small-T-antigen gene. J Virol. 1983 Jan;45(1):73–79. doi: 10.1128/jvi.45.1.73-79.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Miyamura T., Yoshiike K., Takemoto K. K. Characterization of JC papovavirus adapted to growth in human embryonic kidney cells. J Virol. 1980 Aug;35(2):498–504. doi: 10.1128/jvi.35.2.498-504.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Padgett B. L., Rogers C. M., Walker D. L. JC virus, a human polyomavirus associated with progressive multifocal leukoencephalopathy: additional biological characteristics and antigenic relationships. Infect Immun. 1977 Feb;15(2):656–662. doi: 10.1128/iai.15.2.656-662.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Padgett B. L., Walker D. L. Prevalence of antibodies in human sera against JC virus, an isolate from a case of progressive multifocal leukoencephalopathy. J Infect Dis. 1973 Apr;127(4):467–470. doi: 10.1093/infdis/127.4.467. [DOI] [PubMed] [Google Scholar]
  28. Padgett B. L., Walker D. L., ZuRhein G. M., Eckroade R. J., Dessel B. H. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet. 1971 Jun 19;1(7712):1257–1260. doi: 10.1016/s0140-6736(71)91777-6. [DOI] [PubMed] [Google Scholar]
  29. Peden K. W., Pipas J. M., Pearson-White S., Nathans D. Isolation of mutants of an animal virus in bacteria. Science. 1980 Sep 19;209(4463):1392–1396. doi: 10.1126/science.6251547. [DOI] [PubMed] [Google Scholar]
  30. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  31. Shaul Y., Laub O., Walker M. D., Rutter W. J. Homologous recombination between a defective virus and a chromosomal sequence in mammalian cells. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3781–3784. doi: 10.1073/pnas.82.11.3781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Sompayrac L. M., Danna K. J. Efficient infection of monkey cells with DNA of simian virus 40. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7575–7578. doi: 10.1073/pnas.78.12.7575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  34. Takemoto K. K., Howley P. M., Miyamura T. JC human papovavirus replication in human amnion cells. J Virol. 1979 Apr;30(1):384–389. doi: 10.1128/jvi.30.1.384-389.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Tjian R. Protein-DNA interactions at the origin of simian virus 40 DNA replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):655–661. doi: 10.1101/sqb.1979.043.01.073. [DOI] [PubMed] [Google Scholar]
  36. Tyndall C., La Mantia G., Thacker C. M., Favaloro J., Kamen R. A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication. Nucleic Acids Res. 1981 Dec 11;9(23):6231–6250. doi: 10.1093/nar/9.23.6231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Wigler M., Pellicer A., Silverstein S., Axel R. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell. 1978 Jul;14(3):725–731. doi: 10.1016/0092-8674(78)90254-4. [DOI] [PubMed] [Google Scholar]
  38. Wold W. S., Green M., Mackey J. K., Martin J. D., Padgett B. L., Walker D. L. Integration pattern of human JC virus sequences in two clones of a cell line established from a JC virus-induced hamster brain tumor. J Virol. 1980 Mar;33(3):1225–1228. doi: 10.1128/jvi.33.3.1225-1228.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES