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. 1987 Mar;61(3):840–844. doi: 10.1128/jvi.61.3.840-844.1987

Resolution of a polyomavirus-mouse hybrid replicon: release of genomic viral DNA.

A Piché, P Bourgaux
PMCID: PMC254027  PMID: 3027409

Abstract

RmI is a circular chimera containing 1.03 copies of polyomavirus DNA and 1,628 base pairs of mouse DNA, joined through direct and inverted repeat sequences. It is excised from the chromosome of a transformed cell via a site-specific recombination event that is dependent on the activation of the viral gene coding for large T antigen. RmI is shown here to be highly infectious for normal mouse cells. This infectivity reflects the ability of RmI to effectively yield unit-length viral DNA via intramolecular recombination. The effectiveness with which infectious viral DNA is produced from RmI is consistent with the idea that the underlying recombination event is site specific, rather than homologous or illegitimate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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