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. 1987 Mar;61(3):912–915. doi: 10.1128/jvi.61.3.912-915.1987

Inhibition of Cleavage of a Plant Viral Polyprotein by an Inhibitor Activity Present in Wheat Germ and Cowpea Embryos †

Ding S Shih 1,*, Ming Bu 1, Mary A Price 1, Chao-Yun T Shih 2
PMCID: PMC254037  PMID: 16789263

Abstract

In rabbit reticulocyte lysate, the bottom component RNA of cowpea mosaic virus directs the synthesis of a 200,000-molecular-weight precursor protein (200K protein) that is cleaved during synthesis by a reticulocyte enzyme to form a 32K protein and a 170K protein. Cleavage of the 200K protein was found to be effectively inhibited by inhibitor activity in wheat germ and cowpea embryo extracts. The inhibitor was nondialyzable, precipitatable by ammonium sulfate, and partially stable at high temperatures. The activity appeared to be specific in that it caused no inhibition of the secondary cleavage reactions (cleavage of the 170K protein) at concentrations that were sufficient to cause complete inhibition of the primary cleavage reaction (cleavage of the 200K protein).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Daubert S. D., Bruening G., Najarian R. C. Protein bound to the genome RNAs of cowpea mosaic virus. Eur J Biochem. 1978 Dec 1;92(1):45–51. doi: 10.1111/j.1432-1033.1978.tb12721.x. [DOI] [PubMed] [Google Scholar]
  2. Dorssers L., van der Krol S., van der Meer J., van Kammen A., Zabel P. Purification of cowpea mosaic virus RNA replication complex: Identification of a virus-encoded 110,000-dalton polypeptide responsible for RNA chain elongation. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1951–1955. doi: 10.1073/pnas.81.7.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Franssen H., Goldbach R., Broekhuijsen M., Moerman M., van Kammen A. Expression of Middle-Component RNA of Cowpea Mosaic Virus: In Vitro Generation of a Precursor to Both Capsid Proteins by a Bottom-Component RNA-Encoded Protease from Infected Cells. J Virol. 1982 Jan;41(1):8–17. doi: 10.1128/jvi.41.1.8-17.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Franssen H., Moerman M., Rezelman G., Goldbach R. Evidence That the 32,000-Dalton Protein Encoded by Bottom-Component RNA of Cowpea Mosaic Virus is a Proteolytic Processing Enzyme. J Virol. 1984 Apr;50(1):183–190. doi: 10.1128/jvi.50.1.183-190.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldbach R., Rezelman G., Zabel P., van Kammen A. Expression of the Bottom-Component RNA of Cowpea Mosaic Virus: Evidence that the 60-Kilodalton VPg Precursor Is Cleaved into Single VPg and a 58-Kilodalton Polypeptide. J Virol. 1982 May;42(2):630–635. doi: 10.1128/jvi.42.2.630-635.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gopo J. M., Frist R. H. Location of the gene specifying the smaller protein of the cowpea mosaic virus capsid. Virology. 1977 Jun 15;79(2):259–266. doi: 10.1016/0042-6822(77)90353-1. [DOI] [PubMed] [Google Scholar]
  7. Kiefer M. C., Bruening G., Russell M. L. RNA and capsid accumulation in cowpea protoplasts that are resistant to cowpea mosaic virus strain SB. Virology. 1984 Sep;137(2):371–381. doi: 10.1016/0042-6822(84)90229-0. [DOI] [PubMed] [Google Scholar]
  8. Lomonossoff G. P., Shanks M. The nucleotide sequence of cowpea mosaic virus B RNA. EMBO J. 1983;2(12):2253–2258. doi: 10.1002/j.1460-2075.1983.tb01731.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pelham H. R. Synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates. Virology. 1979 Jul 30;96(2):463–477. doi: 10.1016/0042-6822(79)90104-1. [DOI] [PubMed] [Google Scholar]
  10. Peng X. X., Shih D. S. Proteolytic processing of the proteins translated from the bottom component RNA of cowpea mosaic virus. The primary and secondary cleavage reactions. J Biol Chem. 1984 Mar 10;259(5):3197–3201. [PubMed] [Google Scholar]
  11. Rezelman G., Goldbach R., Van Kammen A. Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts. J Virol. 1980 Nov;36(2):366–373. doi: 10.1128/jvi.36.2.366-373.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shih D. S., Shih C. T., Kew O., Pallansch M., Rueckert R., Kaesberg P. Cell-free synthesis and processing of the proteins of poliovirus. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5807–5811. doi: 10.1073/pnas.75.12.5807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stanley J., Rottier P., Davies J. W., Zabel P., Van Kammen A. A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome. Nucleic Acids Res. 1978 Dec;5(12):4505–4522. doi: 10.1093/nar/5.12.4505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tian Y. C., Shih D. S. Cleavage of a viral polyprotein by a cellular proteolytic activity. J Virol. 1986 Feb;57(2):547–551. doi: 10.1128/jvi.57.2.547-551.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. el-Manna M. M., Bruening G. Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus. Virology. 1973 Nov;56(1):198–206. doi: 10.1016/0042-6822(73)90299-7. [DOI] [PubMed] [Google Scholar]
  16. van Kammen A. Purification and properties of the components of cowpea mosaic virus. Virology. 1967 Apr;31(4):633–642. doi: 10.1016/0042-6822(67)90192-4. [DOI] [PubMed] [Google Scholar]
  17. van Wezenbeek P., Verver J., Harmsen J., Vos P., van Kammen A. Primary structure and gene organization of the middle-component RNA of cowpea mosaic virus. EMBO J. 1983;2(6):941–946. doi: 10.1002/j.1460-2075.1983.tb01525.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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