Abstract
The oligomers formed by a mutant nonkaryophilic large T antigen of simian virus 40, which lacks residues 110 through 152 of normal large T antigen and transforms only established cells (L. Fischer-Fantuzzi and C. Vesco, Proc. Natl. Acad. Sci. USA 82:1891-1895, 1985), were found to consist predominantly of dimers. Anti-p53 antibodies precipitated 14 to 16S complexes containing the mutant nonkaryophilic large T antigen and p53 from extracts of transformed cells, and anti-p53 indirect immunofluorescence stained these cells in the cytoplasm.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baumann E. A., Hand R. Phosphorylation and dephosphorylation alter the structure of D2 hybrid T antigen. J Virol. 1982 Oct;44(1):78–87. doi: 10.1128/jvi.44.1.78-87.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bradley M. K., Griffin J. D., Livingston D. M. Relationship of oligomerization to enzymatic and DNA-binding properties of the SV40 large T antigen. Cell. 1982 Jan;28(1):125–134. doi: 10.1016/0092-8674(82)90382-8. [DOI] [PubMed] [Google Scholar]
- Burger C., Fanning E. Specific DNA binding activity of T antigen subclasses varies among different SV40-transformed cell lines. Virology. 1983 Apr 15;126(1):19–31. doi: 10.1016/0042-6822(83)90459-2. [DOI] [PubMed] [Google Scholar]
- Crawford L., Leppard K., Lane D., Harlow E. Cellular proteins reactive with monoclonal antibodies directed against simian virus 40 T-antigen. J Virol. 1982 May;42(2):612–620. doi: 10.1128/jvi.42.2.612-620.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fanning E., Nowak B., Burger C. Detection and characterization of multiple forms of simian virus 40 large T antigen. J Virol. 1981 Jan;37(1):92–102. doi: 10.1128/jvi.37.1.92-102.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fischer-Fantuzzi L., Scheidtmann K. H., Vesco C. Biochemical properties of a transforming nonkaryophilic T antigen of SV40. Virology. 1986 Aug;153(1):87–95. doi: 10.1016/0042-6822(86)90010-3. [DOI] [PubMed] [Google Scholar]
- Fischer-Fantuzzi L., Vesco C. Deletion of 43 amino acids in the NH2-terminal half of the large tumor antigen of simian virus 40 results in a non-karyophilic protein capable of transforming established cells. Proc Natl Acad Sci U S A. 1985 Apr;82(7):1891–1895. doi: 10.1073/pnas.82.7.1891. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freed M. I., Lubin I., Simmons D. T. Stoichiometry of large T antigen and pp53 in complexes isolated from simian virus 40-transformed rat cells. J Virol. 1983 Jun;46(3):1061–1065. doi: 10.1128/jvi.46.3.1061-1065.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greenspan D. S., Carroll R. B. Complex of simian virus 40 large tumor antigen and 48,000-dalton host tumor antigen. Proc Natl Acad Sci U S A. 1981 Jan;78(1):105–109. doi: 10.1073/pnas.78.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gurney E. G., Harrison R. O., Fenno J. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol. 1980 Jun;34(3):752–763. doi: 10.1128/jvi.34.3.752-763.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harlow E., Crawford L. V., Pim D. C., Williamson N. M. Monoclonal antibodies specific for simian virus 40 tumor antigens. J Virol. 1981 Sep;39(3):861–869. doi: 10.1128/jvi.39.3.861-869.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuchino T., Yamaguchi N. Characterization of T antigen in cells infected with a temperature-sensitive mutant of simian virus 40. J Virol. 1975 Jun;15(6):1302–1307. doi: 10.1128/jvi.15.6.1302-1307.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lane D. P., Crawford L. V. T antigen is bound to a host protein in SV40-transformed cells. Nature. 1979 Mar 15;278(5701):261–263. doi: 10.1038/278261a0. [DOI] [PubMed] [Google Scholar]
- Lanford R. E., Butel J. S. Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen. Cell. 1984 Jul;37(3):801–813. doi: 10.1016/0092-8674(84)90415-x. [DOI] [PubMed] [Google Scholar]
- Linzer D. I., Levine A. J. Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells. Cell. 1979 May;17(1):43–52. doi: 10.1016/0092-8674(79)90293-9. [DOI] [PubMed] [Google Scholar]
- McCormick F., Harlow E. Association of a murine 53,000-dalton phosphoprotein with simian virus 40 large-T antigen in transformed cells. J Virol. 1980 Apr;34(1):213–224. doi: 10.1128/jvi.34.1.213-224.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Montenarh M., Henning R. Self-assembly of simian virus 40 large T antigen oligomers by divalent cations. J Virol. 1983 Feb;45(2):531–538. doi: 10.1128/jvi.45.2.531-538.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Montenarh M., Kohler M., Aggeler G., Henning R. Structural prerequisites of simian virus 40 large T antigen for the maintenance of cell transformation. EMBO J. 1985 Nov;4(11):2941–2947. doi: 10.1002/j.1460-2075.1985.tb04027.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Montenarh M., Kohler M., Henning R. Oligomerization of simian virus 40 large T antigen is not necessarily repressed by temperature-sensitive A gene lesions. J Virol. 1984 Mar;49(3):658–664. doi: 10.1128/jvi.49.3.658-664.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Montenarh M., Vesco C., Kemmerling G., Müller D., Henning R. Regions of SV40 large T antigen necessary for oligomerization and complex formation with the cellular oncoprotein p53. FEBS Lett. 1986 Aug 11;204(1):51–55. doi: 10.1016/0014-5793(86)81386-2. [DOI] [PubMed] [Google Scholar]
- Myers R. M., Williams R. C., Tjian R. Oligomeric structure of a simian virus 40 T antigen in free form and bound to DNA. J Mol Biol. 1981 Jun 5;148(4):347–353. doi: 10.1016/0022-2836(81)90180-7. [DOI] [PubMed] [Google Scholar]
- Oren M., Maltzman W., Levine A. J. Post-translational regulation of the 54K cellular tumor antigen in normal and transformed cells. Mol Cell Biol. 1981 Feb;1(2):101–110. doi: 10.1128/mcb.1.2.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prives C., Beck Y., Gidoni D., Oren M., Shure H. DNA binding and sedimentation properties of SV40 T antigens synthesized in vivo and in vitro. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):123–130. doi: 10.1101/sqb.1980.044.01.014. [DOI] [PubMed] [Google Scholar]
- Rotter V., Abutbul H., Ben-Ze'ev A. P53 transformation-related protein accumulates in the nucleus of transformed fibroblasts in association with the chromatin and is found in the cytoplasm of non-transformed fibroblasts. EMBO J. 1983;2(7):1041–1047. doi: 10.1002/j.1460-2075.1983.tb01543.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Santos M., Butel J. S. Dynamic nature of the association of large tumor antigen and p53 cellular protein with the surfaces of simian virus 40-transformed cells. J Virol. 1984 Jan;49(1):50–56. doi: 10.1128/jvi.49.1.50-56.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scheidtmann K. H., Hardung M., Echle B., Walter G. DNA-binding activity of simian virus 40 large T antigen correlates with a distinct phosphorylation state. J Virol. 1984 Apr;50(1):1–12. doi: 10.1128/jvi.50.1.1-12.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scheidtmann K. H., Schickedanz J., Walter G., Lanford R. E., Butel J. S. Differential phosphorylation of cytoplasmic and nuclear variants of simian virus 40 large T antigen encoded by simian virus 40-adenovirus 7 hybrid viruses. J Virol. 1984 May;50(2):636–640. doi: 10.1128/jvi.50.2.636-640.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schickedanz J., Scheidtmann K. H., Walter G. Kinetics of nuclear transport and oligomerization of simian virus 40 large T antigen. Virology. 1986 Jan 15;148(1):47–57. doi: 10.1016/0042-6822(86)90402-2. [DOI] [PubMed] [Google Scholar]
- Schürmann C., Montenarh M., Kohler M., Henning R. Oligomerization of simian virus 40 tumor antigen may be involved in viral DNA replication. Virology. 1985 Oct 15;146(1):1–11. doi: 10.1016/0042-6822(85)90047-9. [DOI] [PubMed] [Google Scholar]
- Van Roy F., Fransen L., Fiers W. Protein kinase activities in immune complexes of simian virus 40 large T-antigen and transformation-associated cellular p53 protein. Mol Cell Biol. 1984 Feb;4(2):232–239. doi: 10.1128/mcb.4.2.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wachter M., Riedle G., Henning R. Functional implications of oligomerization of simian virus 40 large T antigen during lytic virus infection. J Virol. 1985 Nov;56(2):520–526. doi: 10.1128/jvi.56.2.520-526.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]



