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. 1987 May;61(5):1540–1545. doi: 10.1128/jvi.61.5.1540-1545.1987

Structural characteristics of the Corynebacterium lilium bacteriophage CL31.

A Trautwetter, C Blanco, A M Sicard
PMCID: PMC254133  PMID: 3033280

Abstract

Bacteriophage CL31 was isolated on a Corynebacterium lilium strain. Out of 30 strains tested, only CL31 was able to form plaques on Corynebacterium glutamicum ATCC 13287, Brevibacterium lactofermentum ATCC 21086, and Arthrobacter sp. strain SI55, but at a very low frequency. This phage belongs to group B of Bradley's classification (D. E. Bradley, Bacteriol. Rev. 31:230-314; 1967). Its head is 53 nm in diameter, and its tail is 396 nm in length. The phage capsid contains three major proteins, of 12.5, 29.0, and 37.0 kilodaltons, and five minor ones (23.9, 26.0, 27.0, 40.0, and 55.4 kilodaltons). CL31 DNA is a linear molecule of 48 kilobases with cohesive ends. Restriction mapping was performed for endonucleases BglII, EcoRI, SalI, and KpnI. The expression of CL31 genes in Escherichia coli was studied by the maxicell technique; 12 different proteins were detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. An G., Friesen J. D. Plasmid vehicles for direct cloning of Escherichia coli promoters. J Bacteriol. 1979 Nov;140(2):400–407. doi: 10.1128/jb.140.2.400-407.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  4. Bradley D. E. Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967 Dec;31(4):230–314. doi: 10.1128/br.31.4.230-314.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brandsch R., Faller W., Schneider K. Plasmid pAO1 of Arthrobacter oxidans encodes 6-hydroxy-D-nicotine oxidase: cloning and expression of the gene in Escherichia coli. Mol Gen Genet. 1986 Jan;202(1):96–101. doi: 10.1007/BF00330523. [DOI] [PubMed] [Google Scholar]
  6. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Espejo R. T., Canelo E. S. Properties of bacteriophage PM2: a lipid-containing bacterial virus. Virology. 1968 Apr;34(4):738–747. doi: 10.1016/0042-6822(68)90094-9. [DOI] [PubMed] [Google Scholar]
  8. Follettie M. T., Sinskey A. J. Molecular cloning and nucleotide sequence of the Corynebacterium glutamicum pheA gene. J Bacteriol. 1986 Aug;167(2):695–702. doi: 10.1128/jb.167.2.695-702.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fukui S., Tanaka A. Immobilized microbial cells. Annu Rev Microbiol. 1982;36:145–172. doi: 10.1146/annurev.mi.36.100182.001045. [DOI] [PubMed] [Google Scholar]
  10. Krüger D. H., Hansen S., Schroeder C. Virus adaptation to host cells: the non-classical modification of phage T3. Z Allg Mikrobiol. 1980;20(8):495–502. [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  13. Miwa K., Matsui K., Terabe M., Ito K., Ishida M., Takagi H., Nakamori S., Sano K. Construction of novel shuttle vectors and a cosmid vector for the glutamic acid-producing bacteria Brevibacterium lactofermentum and Corynebacterium glutamicum. Gene. 1985;39(2-3):281–286. doi: 10.1016/0378-1119(85)90324-5. [DOI] [PubMed] [Google Scholar]
  14. Márquez G., Sousa J. M., Sánchez F. Cloning and expression in Escherichia coli of genes involved in the lysine pathway of Brevibacterium lactofermentum. J Bacteriol. 1985 Oct;164(1):379–383. doi: 10.1128/jb.164.1.379-383.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Oakley B. R., Kirsch D. R., Morris N. R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980 Jul 1;105(2):361–363. doi: 10.1016/0003-2697(80)90470-4. [DOI] [PubMed] [Google Scholar]
  16. Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  18. Rosner A., Gutstein R., Aviv H. Isolation of viable deletion mutants of Streptomyces actinophage (Pal 6) and their molecular characterization. Mol Gen Genet. 1980;178(2):337–341. doi: 10.1007/BF00270481. [DOI] [PubMed] [Google Scholar]
  19. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  21. Veldkamp H. Saprophytic coryneform bacteria. Annu Rev Microbiol. 1970;24:209–240. doi: 10.1146/annurev.mi.24.100170.001233. [DOI] [PubMed] [Google Scholar]
  22. Vidaver A. K. The plant pathogenic corynebacteria. Annu Rev Microbiol. 1982;36:495–517. doi: 10.1146/annurev.mi.36.100182.002431. [DOI] [PubMed] [Google Scholar]
  23. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]
  24. Yeh P., Oreglia J., Sicard A. M. Transfection of Corynebacterium lilium protoplasts. J Gen Microbiol. 1985 Dec;131(12):3179–3183. doi: 10.1099/00221287-131-12-3179. [DOI] [PubMed] [Google Scholar]

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