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. 1987 May;61(5):1731–1734. doi: 10.1128/jvi.61.5.1731-1734.1987

cDNA cloning with a retrovirus expression vector: generation of a pp60c-src cDNA clone.

P L Kaplan, S Simon, C A Cartwright, W Eckhart
PMCID: PMC254166  PMID: 2437323

Abstract

We used a murine retroviral expression vector, containing a genomic clone of the chicken c-src gene, a bacterial origin of replication, and a selectable marker, to remove 10 introns from the c-src gene. All 10 introns were removed accurately, and no mutations were introduced. The processed gene encoded a functional pp60c-src protein tyrosine kinase.

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Selected References

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