Abstract
We compared the nucleotide sequence in the transcriptional control region of BK virus isolates cloned directly from human urine (BK-WW) with that of prototype BK virus. BK-WW was found to have a 63-base-pair insertion and only one of the 68-base-pair enhancer repeat elements. In transient expression assays, BK-WW enhancer showed approximately one-half the activity given by the prototype enhancer.
Full text
PDF![1747](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73dd/254170/c96aca9b50c4/jvirol00096-0457.png)
![1748](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73dd/254170/ba520d06d25d/jvirol00096-0458.png)
![1749](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73dd/254170/e7ae255fc059/jvirol00096-0459.png)
![1750](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73dd/254170/9810975facd7/jvirol00096-0460.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Benoist C., Chambon P. In vivo sequence requirements of the SV40 early promotor region. Nature. 1981 Mar 26;290(5804):304–310. doi: 10.1038/290304a0. [DOI] [PubMed] [Google Scholar]
- Chauhan S., Lecatsas G., Harley E. H. Genome analysis of BK (WW) viral DNA cloned directly from human urine. Intervirology. 1984;22(3):170–176. doi: 10.1159/000149547. [DOI] [PubMed] [Google Scholar]
- Gardner S. D., Field A. M., Coleman D. V., Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet. 1971 Jun 19;1(7712):1253–1257. doi: 10.1016/s0140-6736(71)91776-4. [DOI] [PubMed] [Google Scholar]
- Gidoni D., Dynan W. S., Tjian R. Multiple specific contacts between a mammalian transcription factor and its cognate promoters. 1984 Nov 29-Dec 5Nature. 312(5993):409–413. doi: 10.1038/312409a0. [DOI] [PubMed] [Google Scholar]
- Gorman C. M., Moffat L. F., Howard B. H. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. doi: 10.1128/mcb.2.9.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
- Gruss P., Dhar R., Khoury G. Simian virus 40 tandem repeated sequences as an element of the early promoter. Proc Natl Acad Sci U S A. 1981 Feb;78(2):943–947. doi: 10.1073/pnas.78.2.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hearing P., Shenk T. The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element. Cell. 1983 Jul;33(3):695–703. doi: 10.1016/0092-8674(83)90012-0. [DOI] [PubMed] [Google Scholar]
- Howley P. M., Mullarkey M. F., Takemoto K. K., Martin M. A. Characterization of human papovavirus BK DNA. J Virol. 1975 Jan;15(1):173–181. doi: 10.1128/jvi.15.1.173-181.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Khoury G., Gruss P. Enhancer elements. Cell. 1983 Jun;33(2):313–314. doi: 10.1016/0092-8674(83)90410-5. [DOI] [PubMed] [Google Scholar]
- Mew R. T., Lecatsas G., Prozesky O. W., Harley E. H. Characteristics of BK papovavirus DNA prepared directly from human urine. Intervirology. 1981;16(1):14–19. doi: 10.1159/000149242. [DOI] [PubMed] [Google Scholar]
- Pagnani M., Negrini M., Reschiglian P., Corallini A., Balboni P. G., Scherneck S., Macino G., Milanesi G., Barbanti-Brodano G. Molecular and biological properties of BK virus-IR, a BK virus variant isolated from a human tumor. J Virol. 1986 Aug;59(2):500–505. doi: 10.1128/jvi.59.2.500-505.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pater A., Pater M. M., Chang L. S., Slawin K., Di Mayorca G. Multiple origins of the complementary defective genomes of RF and origin proximal sequences of GS, two human papovavirus isolates. Virology. 1983 Dec;131(2):426–436. doi: 10.1016/0042-6822(83)90509-3. [DOI] [PubMed] [Google Scholar]
- Rosenthal N., Kress M., Gruss P., Khoury G. BK viral enhancer element and a human cellular homolog. Science. 1983 Nov 18;222(4625):749–755. doi: 10.1126/science.6314501. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seif I., Khoury G., Dhar R. The genome of human papovavirus BKV. Cell. 1979 Dec;18(4):963–977. doi: 10.1016/0092-8674(79)90209-5. [DOI] [PubMed] [Google Scholar]
- Watanabe S., Yoshiike K. Change of DNA near the origin of replication enhances the transforming capacity of human papovavirus BK. J Virol. 1982 Jun;42(3):978–985. doi: 10.1128/jvi.42.3.978-985.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watanabe S., Yoshiike K. Evolutionary changes of transcriptional control region in a minute-plaque viable deletion mutant of BK virus. J Virol. 1986 Aug;59(2):260–266. doi: 10.1128/jvi.59.2.260-266.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yang R. C., Wu R. BK virus DNA: complete nucleotide sequence of a human tumor virus. Science. 1979 Oct 26;206(4417):456–462. doi: 10.1126/science.228391. [DOI] [PubMed] [Google Scholar]
- Yang R. C., Wu R. Comparative study of papovavirus DNA: BKV(MM), BKV(WT) and SV40. Nucleic Acids Res. 1979 Oct 10;7(3):651–668. doi: 10.1093/nar/7.3.651. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zenke M., Grundström T., Matthes H., Wintzerith M., Schatz C., Wildeman A., Chambon P. Multiple sequence motifs are involved in SV40 enhancer function. EMBO J. 1986 Feb;5(2):387–397. doi: 10.1002/j.1460-2075.1986.tb04224.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ter Schegget J., Sol C. J., Baan E. W., van der Noordaa J., van Ormondt H. Naturally occurring BK virus variants (JL and Dik) with deletions in the putative early enhancer-promoter sequences. J Virol. 1985 Jan;53(1):302–305. doi: 10.1128/jvi.53.1.302-305.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]