Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Virology logoLink to Journal of Virology
. 1987 Jun;61(6):1834–1841. doi: 10.1128/jvi.61.6.1834-1841.1987

Evidence that the sigma 1 protein of reovirus serotype 3 is a multimer.

R Bassel-Duby, M L Nibert, C J Homcy, B N Fields, D G Sawutz
PMCID: PMC254187  PMID: 3553613

Abstract

In this report, we study the reovirus serotype 3 (strain Dearing) sigma 1 protein obtained from various sources: from Escherichia coli expressing sigma 1 protein, from reovirus-infected mouse L cells, and from purified reovirions. We demonstrate that the sigma 1 protein is a multimer in its undisrupted form and present biochemical evidence suggesting that the multimer is made up of four sigma 1 subunits.

Full text

PDF
1834

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amann E., Brosius J., Ptashne M. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene. 1983 Nov;25(2-3):167–178. doi: 10.1016/0378-1119(83)90222-6. [DOI] [PubMed] [Google Scholar]
  2. Bartlett N. M., Gillies S. C., Bullivant S., Bellamy A. R. Electron microscopy study of reovirus reaction cores. J Virol. 1974 Aug;14(2):315–326. doi: 10.1128/jvi.14.2.315-326.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bassel-Duby R., Jayasuriya A., Chatterjee D., Sonenberg N., Maizel J. V., Jr, Fields B. N. Sequence of reovirus haemagglutinin predicts a coiled-coil structure. 1985 May 30-Jun 5Nature. 315(6018):421–423. doi: 10.1038/315421a0. [DOI] [PubMed] [Google Scholar]
  4. Bazinet C., King J. The DNA translocating vertex of dsDNA bacteriophage. Annu Rev Microbiol. 1985;39:109–129. doi: 10.1146/annurev.mi.39.100185.000545. [DOI] [PubMed] [Google Scholar]
  5. Burstin S. J., Spriggs D. R., Fields B. N. Evidence for functional domains on the reovirus type 3 hemagglutinin. Virology. 1982 Feb;117(1):146–155. doi: 10.1016/0042-6822(82)90514-1. [DOI] [PubMed] [Google Scholar]
  6. Cashdollar L. W., Chmelo R. A., Wiener J. R., Joklik W. K. Sequences of the S1 genes of the three serotypes of reovirus. Proc Natl Acad Sci U S A. 1985 Jan;82(1):24–28. doi: 10.1073/pnas.82.1.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  8. Co M. S., Gaulton G. N., Fields B. N., Greene M. I. Isolation and biochemical characterization of the mammalian reovirus type 3 cell-surface receptor. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1494–1498. doi: 10.1073/pnas.82.5.1494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cohen C., Phillips G. N., Jr Spikes and fimbriae: alpha-helical proteins form surface projections on microorganisms. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5303–5304. doi: 10.1073/pnas.78.9.5303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cohen C., Reinhardt B., Parry D. A., Roelants G. E., Hirsch W., Kanwé B. Alpha-helical coiled-coil structures of Trypanosoma brucei variable surface glycoproteins. Nature. 1984 Sep 13;311(5982):169–171. doi: 10.1038/311169a0. [DOI] [PubMed] [Google Scholar]
  11. Davies G. E., Stark G. R. Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins. Proc Natl Acad Sci U S A. 1970 Jul;66(3):651–656. doi: 10.1073/pnas.66.3.651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Drayna D., Fields B. N. Biochemical studies on the mechanism of chemical and physical inactivation of reovirus. J Gen Virol. 1982 Nov;63(Pt 1):161–170. doi: 10.1099/0022-1317-63-1-161. [DOI] [PubMed] [Google Scholar]
  13. Drayna D., Fields B. N. Genetic studies on the mechanism of chemical and physical inactivation of reovirus. J Gen Virol. 1982 Nov;63(Pt 1):149–159. doi: 10.1099/0022-1317-63-1-149. [DOI] [PubMed] [Google Scholar]
  14. Fields B. N., Greene M. I. Genetic and molecular mechanisms of viral pathogenesis: implications for prevention and treatment. Nature. 1982 Nov 4;300(5887):19–23. doi: 10.1038/300019a0. [DOI] [PubMed] [Google Scholar]
  15. Finberg R., Weiner H. L., Fields B. N., Benacerraf B., Burakoff S. J. Generation of cytolytic T lymphocytes after reovirus infection: role of S1 gene. Proc Natl Acad Sci U S A. 1979 Jan;76(1):442–446. doi: 10.1073/pnas.76.1.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Fontana A., Weiner H. L. Interaction of reovirus with cell surface receptors. II. Generation of suppressor T cells by the hemagglutinin of reovirus type 3. J Immunol. 1980 Dec;125(6):2660–2664. [PubMed] [Google Scholar]
  17. Freymann D. M., Metcalf P., Turner M., Wiley D. C. 6 A-resolution X-ray structure of a variable surface glycoprotein from Trypanosoma brucei. Nature. 1984 Sep 13;311(5982):167–169. doi: 10.1038/311167a0. [DOI] [PubMed] [Google Scholar]
  18. Hendrix R. W. Symmetry mismatch and DNA packaging in large bacteriophages. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4779–4783. doi: 10.1073/pnas.75.10.4779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hogle J. M., Chow M., Filman D. J. Three-dimensional structure of poliovirus at 2.9 A resolution. Science. 1985 Sep 27;229(4720):1358–1365. doi: 10.1126/science.2994218. [DOI] [PubMed] [Google Scholar]
  20. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  21. Lee P. W., Hayes E. C., Joklik W. K. Protein sigma 1 is the reovirus cell attachment protein. Virology. 1981 Jan 15;108(1):156–163. doi: 10.1016/0042-6822(81)90535-3. [DOI] [PubMed] [Google Scholar]
  22. McLachlan A. D., Karn J. Periodic charge distributions in the myosin rod amino acid sequence match cross-bridge spacings in muscle. Nature. 1982 Sep 16;299(5880):226–231. doi: 10.1038/299226a0. [DOI] [PubMed] [Google Scholar]
  23. Nagata L., Masri S. A., Mah D. C., Lee P. W. Molecular cloning and sequencing of the reovirus (serotype 3) S1 gene which encodes the viral cell attachment protein sigma 1. Nucleic Acids Res. 1984 Nov 26;12(22):8699–8710. doi: 10.1093/nar/12.22.8699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Onodera T., Toniolo A., Ray U. R., Jenson A. B., Knazek R. A., Notkins A. L. Virus-induced diabetes mellitus. XX. Polyendocrinopathy and autoimmunity. J Exp Med. 1981 Jun 1;153(6):1457–1473. doi: 10.1084/jem.153.6.1457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pelletier J., Nicholson R., Bassel-Duby R., Fields B. N., Sonenberg N. Expression of reovirus type 3 (Dearing) sigma 1 and sigma s polypeptides in Escherichia coli. J Gen Virol. 1987 Jan;68(Pt 1):135–145. doi: 10.1099/0022-1317-68-1-135. [DOI] [PubMed] [Google Scholar]
  26. Phillips G. N., Jr, Flicker P. F., Cohen C., Manjula B. N., Fischetti V. A. Streptococcal M protein: alpha-helical coiled-coil structure and arrangement on the cell surface. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4689–4693. doi: 10.1073/pnas.78.8.4689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ralph S. J., Harvey J. D., Bellamy A. R. Subunit structure of the reovirus spike. J Virol. 1980 Dec;36(3):894–896. doi: 10.1128/jvi.36.3.894-896.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H. J., Johnson J. E., Kamer G., Luo M., Mosser A. G. Structure of a human common cold virus and functional relationship to other picornaviruses. Nature. 1985 Sep 12;317(6033):145–153. doi: 10.1038/317145a0. [DOI] [PubMed] [Google Scholar]
  29. Shepherd W. D., Kaplan S. Effect of heat and 2-mercaptoethanol on intracytoplasmic membrane polypeptides of Rhodopseudomonas sphaeroides. J Bacteriol. 1978 Aug;135(2):656–667. doi: 10.1128/jb.135.2.656-667.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]
  31. Steinert P. M., Steven A. C., Roop D. R. The molecular biology of intermediate filaments. Cell. 1985 Sep;42(2):411–420. doi: 10.1016/0092-8674(85)90098-4. [DOI] [PubMed] [Google Scholar]
  32. Tyler K. L., McHenry L. C., Jr Classics in neurology. Fragments of neurologic history: pseudohypertrophic muscular dystrophy and Gowers' sign. Neurology. 1983 Jan;33(1):88–89. doi: 10.1212/wnl.33.1.88. [DOI] [PubMed] [Google Scholar]
  33. Tyler K. L., McPhee D. A., Fields B. N. Distinct pathways of viral spread in the host determined by reovirus S1 gene segment. Science. 1986 Aug 15;233(4765):770–774. doi: 10.1126/science.3016895. [DOI] [PubMed] [Google Scholar]
  34. Weber P. C., Salemme F. R. Structural and functional diversity in 4-alpha-helical proteins. Nature. 1980 Sep 4;287(5777):82–84. doi: 10.1038/287082a0. [DOI] [PubMed] [Google Scholar]
  35. Weiner H. L., Ault K. A., Fields B. N. Interaction of reovirus with cell surface receptors. I. Murine and human lymphocytes have a receptor for the hemagglutinin of reovirus type 3. J Immunol. 1980 May;124(5):2143–2148. [PubMed] [Google Scholar]
  36. Weiner H. L., Drayna D., Averill D. R., Jr, Fields B. N. Molecular basis of reovirus virulence: role of the S1 gene. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5744–5748. doi: 10.1073/pnas.74.12.5744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Weiner H. L., Fields B. N. Neutralization of reovirus: the gene responsible for the neutralization antigen. J Exp Med. 1977 Nov 1;146(5):1305–1310. doi: 10.1084/jem.146.5.1305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Weiner H. L., Greene M. I., Fields B. N. Delayed hypersensitivity in mice infected with reovirus. I. Identification of host and viral gene products responsible for the immune response. J Immunol. 1980 Jul;125(1):278–282. [PubMed] [Google Scholar]
  39. Weiner H. L., Powers M. L., Fields B. N. Absolute linkage of virulence and central nervous system cell tropism of reoviruses to viral hemagglutinin. J Infect Dis. 1980 May;141(5):609–616. doi: 10.1093/infdis/141.5.609. [DOI] [PubMed] [Google Scholar]
  40. Weiner H. L., Ramig R. F., Mustoe T. A., Fields B. N. Identification of the gene coding for the hemagglutinin of reovirus. Virology. 1978 May 15;86(2):581–584. doi: 10.1016/0042-6822(78)90099-5. [DOI] [PubMed] [Google Scholar]
  41. Wilson I. A., Skehel J. J., Wiley D. C. Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution. Nature. 1981 Jan 29;289(5796):366–373. doi: 10.1038/289366a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES