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. 1987 Jun;61(6):1938–1948. doi: 10.1128/jvi.61.6.1938-1948.1987

Creation of a chimeric oncogene: analysis of the biochemical and biological properties of v-erbB/src fusion polypeptide.

M L Privalsky
PMCID: PMC254201  PMID: 2883328

Abstract

A novel gene was created that linked complementary portions of two different tyrosine kinase oncogenes: v-erB and v-src. The v-erbB/src chimera encoded a glycoprotein exhibiting the subcellular distribution of the v-erbB protein but containing the kinase catalytic domain of the v-src parent. Fibroblasts expressing the v-erbB/src gene product became transformed to an oncogenic state and closely resembled cells expressing the v-erbB parent oncogene. Our results indicated that v-erbB sequences can be functionally replaced by sequences derived from a different oncogene, v-src, and that important determinants of the transformed phenotype appear to be encoded in oncogene sequences distinct from those defining the kinase catalytic domain itself.

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Selected References

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