Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1987 Jun;61(6):2059–2062. doi: 10.1128/jvi.61.6.2059-2062.1987

Stimulation of expression of the human endogenous retrovirus genome by female steroid hormones in human breast cancer cell line T47D.

M Ono, M Kawakami, H Ushikubo
PMCID: PMC254220  PMID: 2883329

Abstract

Expression of the human endogenous retrovirus genome HERV-K, homologous to mouse mammary tumor virus, was investigated in cultured human tumor cells. In several cell lines, the HERV-K genome was expressed as an 8.8-kilobase poly(A)+ RNA which appeared to be a full-size transcript of this genome. In the human breast cancer cell line T47D, stimulation of HERV-K genome expression by progesterone was observed after estradiol treatment.

Full text

PDF
2059

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Callahan R., Chiu I. M., Wong J. F., Tronick S. R., Roe B. A., Aaronson S. A., Schlom J. A new class of endogenous human retroviral genomes. Science. 1985 Jun 7;228(4704):1208–1211. doi: 10.1126/science.2408338. [DOI] [PubMed] [Google Scholar]
  2. Cato A. C., Miksicek R., Schütz G., Arnemann J., Beato M. The hormone regulatory element of mouse mammary tumour virus mediates progesterone induction. EMBO J. 1986 Sep;5(9):2237–2240. doi: 10.1002/j.1460-2075.1986.tb04490.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chandler V. L., Maler B. A., Yamamoto K. R. DNA sequences bound specifically by glucocorticoid receptor in vitro render a heterologous promoter hormone responsive in vivo. Cell. 1983 Jun;33(2):489–499. doi: 10.1016/0092-8674(83)90430-0. [DOI] [PubMed] [Google Scholar]
  4. Deen K. C., Sweet R. W. Murine mammary tumor virus pol-related sequences in human DNA: characterization and sequence comparison with the complete murine mammary tumor virus pol gene. J Virol. 1986 Feb;57(2):422–432. doi: 10.1128/jvi.57.2.422-432.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hynes N., van Ooyen A. J., Kennedy N., Herrlich P., Ponta H., Groner B. Subfragments of the large terminal repeat cause glucocorticoid-responsive expression of mouse mammary tumor virus and of an adjacent gene. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3637–3641. doi: 10.1073/pnas.80.12.3637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Keydar I., Chen L., Karby S., Weiss F. R., Delarea J., Radu M., Chaitcik S., Brenner H. J. Establishment and characterization of a cell line of human breast carcinoma origin. Eur J Cancer. 1979 May;15(5):659–670. doi: 10.1016/0014-2964(79)90139-7. [DOI] [PubMed] [Google Scholar]
  7. Keydar I., Ohno T., Nayak R., Sweet R., Simoni F., Weiss F., Karby S., Mesa-Tejada R., Spiegelman S. Properties of retrovirus-like particles produced by a human breast carcinoma cell line: immunological relationship with mouse mammary tumor virus proteins. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4188–4192. doi: 10.1073/pnas.81.13.4188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mager D. L., Henthorn P. S. Identification of a retrovirus-like repetitive element in human DNA. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7510–7514. doi: 10.1073/pnas.81.23.7510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Noda M., Kurihara M., Takano T. Retrovirus-related sequences in human DNA: detection and cloning of sequences which hybridize with the long terminal repeat of baboon endogenous virus. Nucleic Acids Res. 1982 May 11;10(9):2865–2878. doi: 10.1093/nar/10.9.2865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. O'Connell C., O'Brien S., Nash W. G., Cohen M. ERV3, a full-length human endogenous provirus: chromosomal localization and evolutionary relationships. Virology. 1984 Oct 30;138(2):225–235. doi: 10.1016/0042-6822(84)90347-7. [DOI] [PubMed] [Google Scholar]
  11. Ono M., Cole M. D., White A. T., Huang R. C. Sequence organization of cloned intracisternal A particle genes. Cell. 1980 Sep;21(2):465–473. doi: 10.1016/0092-8674(80)90483-3. [DOI] [PubMed] [Google Scholar]
  12. Ono M. Molecular cloning and long terminal repeat sequences of human endogenous retrovirus genes related to types A and B retrovirus genes. J Virol. 1986 Jun;58(3):937–944. doi: 10.1128/jvi.58.3.937-944.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ono M., Yasunaga T., Miyata T., Ushikubo H. Nucleotide sequence of human endogenous retrovirus genome related to the mouse mammary tumor virus genome. J Virol. 1986 Nov;60(2):589–598. doi: 10.1128/jvi.60.2.589-598.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Repaske R., Steele P. E., O'Neill R. R., Rabson A. B., Martin M. A. Nucleotide sequence of a full-length human endogenous retroviral segment. J Virol. 1985 Jun;54(3):764–772. doi: 10.1128/jvi.54.3.764-772.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ringold G. M. Regulation of mouse mammary tumor virus gene expression by glucocorticoid hormones. Curr Top Microbiol Immunol. 1983;106:79–103. doi: 10.1007/978-3-642-69357-1_4. [DOI] [PubMed] [Google Scholar]
  16. Scheidereit C., Geisse S., Westphal H. M., Beato M. The glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumour virus. Nature. 1983 Aug 25;304(5928):749–752. doi: 10.1038/304749a0. [DOI] [PubMed] [Google Scholar]
  17. Segev N., Hizi A., Kirenberg F., Keydar I. Characterization of a protein, released by the T47D cell line, immunologically related to the major envelope protein of mouse mammary tumor virus. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1531–1535. doi: 10.1073/pnas.82.5.1531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sekiguchi M., Sakakibara K., Fujii G. Establishment of cultured cell lines derived from a human gastric carcinoma. Jpn J Exp Med. 1978 Feb;48(1):61–68. [PubMed] [Google Scholar]
  19. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Westley B., May F. E. The human genome contains multiple sequences of varying homology to mouse mammary tumour virus DNA. Gene. 1984 May;28(2):221–227. doi: 10.1016/0378-1119(84)90259-2. [DOI] [PubMed] [Google Scholar]
  21. von der Ahe D., Janich S., Scheidereit C., Renkawitz R., Schütz G., Beato M. Glucocorticoid and progesterone receptors bind to the same sites in two hormonally regulated promoters. Nature. 1985 Feb 21;313(6004):706–709. doi: 10.1038/313706a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES