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. 1984 Jul;51(1):14–19. doi: 10.1128/jvi.51.1.14-19.1984

Evidence for variation of supercoil densities among simian virus 40 nucleoprotein complexes and for higher supercoil density in replicating complexes.

S S Chen, M T Hsu
PMCID: PMC254392  PMID: 6328033

Abstract

The distribution of DNA topoisomers in intracellular simian virus 40 DNA was analyzed by gel electrophoresis. The results suggested that DNA extracted from 70S chromatin had a different superhelical density distribution as compared with the DNA obtained from virions or virion assembly intermediates. The heterogeneity of simian virus 40 viral DNA superhelical density at a late time after infection was partly due to increased virion production and partly due to the intrinsic heterogeneity of the superhelical density of DNA extracted from virions. Using two-dimensional gel electrophoretic analysis we also showed that simian virus 40 DNA templates used for DNA replication have a higher average superhelical density than the bulk of intracellular viral DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coca-Prados M., Hsu M. T. Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly. J Virol. 1979 Jul;31(1):199–208. doi: 10.1128/jvi.31.1.199-208.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coca-Prados M., Vidali G., Hsu M. T. Intracellular forms of simian virus 40 nucleoprotein complexes. III. Study of histone modifications. J Virol. 1980 Nov;36(2):353–360. doi: 10.1128/jvi.36.2.353-360.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coca-Prados M., Yu H. Y., Hsu M. T. Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion. J Virol. 1982 Nov;44(2):603–609. doi: 10.1128/jvi.44.2.603-609.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Depew D. E., Wang J. C. Conformational fluctuations of DNA helix. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4275–4279. doi: 10.1073/pnas.72.11.4275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fernandez-Munoz R., Coca-Prados M., Hsu M. T. Intracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells. J Virol. 1979 Feb;29(2):612–623. doi: 10.1128/jvi.29.2.612-623.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Germond J. E., Hirt B., Oudet P., Gross-Bellark M., Chambon P. Folding of the DNA double helix in chromatin-like structures from simian virus 40. Proc Natl Acad Sci U S A. 1975 May;72(5):1843–1847. doi: 10.1073/pnas.72.5.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gourlie B. B., Pigiet V. P. Polyoma virus minichromosomes: characterization of the products of in vitro DNA synthesis. J Virol. 1983 Feb;45(2):585–593. doi: 10.1128/jvi.45.2.585-593.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grigoryev S. A., Ioffe L. B. The dependence of the linking number of a circular minichromosome upon the shape and the orientation of its nucleosomes. FEBS Lett. 1981 Jul 20;130(1):43–46. doi: 10.1016/0014-5793(81)80661-8. [DOI] [PubMed] [Google Scholar]
  9. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  10. Keller W. Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4876–4880. doi: 10.1073/pnas.72.12.4876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klug A., Lutter L. C. The helical periodicity of DNA on the nucleosome. Nucleic Acids Res. 1981 Sep 11;9(17):4267–4283. doi: 10.1093/nar/9.17.4267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Luchnik A. N., Bakayev V. V., Zbarsky I. B., Georgiev G. P. Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin. EMBO J. 1982;1(11):1353–1358. doi: 10.1002/j.1460-2075.1982.tb01322.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rinaldy A., Feunteun J., Rosenberg B. H. Prereplicative events involving simian virus 40 DNA in permissive cells. J Virol. 1982 Jan;41(1):237–243. doi: 10.1128/jvi.41.1.237-243.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shure M., Pulleyblank D. E., Vinograd J. The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity. Nucleic Acids Res. 1977;4(5):1183–1205. doi: 10.1093/nar/4.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith G. R. DNA supercoiling: another level for regulating gene expression. Cell. 1981 Jun;24(3):599–600. doi: 10.1016/0092-8674(81)90085-4. [DOI] [PubMed] [Google Scholar]
  16. Stein A. DNA wrapping in nucleosomes. The linking number problem re-examined. Nucleic Acids Res. 1980 Oct 24;8(20):4803–4820. doi: 10.1093/nar/8.20.4803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sundin O., Varshavsky A. Terminal stages of SV40 DNA replication proceed via multiply intertwined catenated dimers. Cell. 1980 Aug;21(1):103–114. doi: 10.1016/0092-8674(80)90118-x. [DOI] [PubMed] [Google Scholar]
  18. Wang J. C. The path of DNA in the nucleosome. Cell. 1982 Jul;29(3):724–726. doi: 10.1016/0092-8674(82)90433-0. [DOI] [PubMed] [Google Scholar]
  19. Worcel A., Strogatz S., Riley D. Structure of chromatin and the linking number of DNA. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1461–1465. doi: 10.1073/pnas.78.3.1461. [DOI] [PMC free article] [PubMed] [Google Scholar]

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