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. 1984 Jul;51(1):47–51. doi: 10.1128/jvi.51.1.47-51.1984

Monoclonal antibodies directed against different antigenic determinants of rotavirus.

J P Lambert, P Marbehant, D Marissens, G Zissis
PMCID: PMC254397  PMID: 6202884

Abstract

We have developed a series of monoclonal antibodies against the calf strain RIT 4237 (subgroup 1) and the human strain 82-561 (subgroup 3) of rotavirus, both grown in tissue culture, and also against the human rotavirus 81-2162 (subgroup 2), extracted from a fecal specimen. A variety of different specificities was observed among these antibodies, namely, antibodies against group and subgroup determinants, as well as neutralizing antibodies. By using monoclonal antibodies against the subgroup antigen in an enzyme-linked immunoassay system, the constant predominance of subgroup 2 viruses in humans was confirmed in 74 stools collected from children in Brussels who suffered a diarrheal illness between July 1981 and June 1983. The availability of these antibodies also made it possible to improve the sensitivity and the specificity of the test system.

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Selected References

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  1. Burtonboy G., Bazin H., Delferriere N. Rat hybridoma antibodies against canine parvovirus. Arch Virol. 1982;71(4):291–302. doi: 10.1007/BF01315059. [DOI] [PubMed] [Google Scholar]
  2. Gary G. W., Jr, Black D. R., Palmer E. Monoclonal IgG to the inner capsid of human rotavirus. Arch Virol. 1982;72(3):223–227. doi: 10.1007/BF01348968. [DOI] [PubMed] [Google Scholar]
  3. Greenberg H. B., Wyatt R. G., Kapikian A. Z., Kalica A. R., Flores J., Jones R. Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment. Infect Immun. 1982 Jul;37(1):104–109. doi: 10.1128/iai.37.1.104-109.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Greenberg H., McAuliffe V., Valdesuso J., Wyatt R., Flores J., Kalica A., Hoshino Y., Singh N. Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infect Immun. 1983 Jan;39(1):91–99. doi: 10.1128/iai.39.1.91-99.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology. 1981 Jul 30;112(2):385–390. doi: 10.1016/0042-6822(81)90285-3. [DOI] [PubMed] [Google Scholar]
  6. Kapikian A. Z., Cline W. L., Greenberg H. B., Wyatt R. G., Kalica A. R., Banks C. E., James H. D., Jr, Flores J., Chanock R. M. Antigenic characterization of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA): evidence for distinctness of IAHA and neutralization antigens. Infect Immun. 1981 Aug;33(2):415–425. doi: 10.1128/iai.33.2.415-425.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kapikian A. Z., Cline W. L., Kim H. W., Kalica A. R., Wyatt R. G., Vankirk D. H., Chanock R. M., James H. D., Jr, Vaughn A. L. Antigenic relationships among five reovirus-like (RVL) agents by complement fixation (CF) and development of new substitute CF antigens for the human RVL agent of infantile gastroenteritis. Proc Soc Exp Biol Med. 1976 Sep;152(4):535–539. doi: 10.3181/00379727-152-39434. [DOI] [PubMed] [Google Scholar]
  8. Lambert J. P., Marissens D., Marbehant P., Zissis G. Prevalence of subgroup 1, 2, and 3 rotaviruses in Belgian children suffering from acute diarrhea (1978-1981). J Med Virol. 1983;11(1):31–38. doi: 10.1002/jmv.1890110105. [DOI] [PubMed] [Google Scholar]
  9. Mebus C. A., Kono M., Underdahl N. R., Twiehaus M. J. Cell culture propagation of neonatal calf diarrhea (scours) virus. Can Vet J. 1971 Mar;12(3):69–72. [PMC free article] [PubMed] [Google Scholar]
  10. Pereira L., Dondero D. V., Gallo D., Devlin V., Woodie J. D. Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies. Infect Immun. 1982 Jan;35(1):363–367. doi: 10.1128/iai.35.1.363-367.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roseto A., Guillemin M. C., Cohen J., Scherrer R., Périés J. Production d'anticorps monoclonaux contre le Rotavirus bovin. C R Seances Acad Sci III. 1982 Jan 25;294(4):211–214. [PubMed] [Google Scholar]
  12. Roseto A., Scherrer R., Cohen J., Guillemin M. C., Charpilienne A., Feynerol C., Peries J. Isolation and characterization of anti-rotavirus immunoglobulins secreted by cloned hybridoma cell lines. J Gen Virol. 1983 Jan;64(Pt 1):237–240. doi: 10.1099/0022-1317-64-1-237. [DOI] [PubMed] [Google Scholar]
  13. Sato K., Inaba Y., Shinozaki T., Fujii R., Matumoto M. Isolation of human rotavirus in cell cultures: brief report. Arch Virol. 1981;69(2):155–160. doi: 10.1007/BF01315159. [DOI] [PubMed] [Google Scholar]
  14. Sonza S., Breschkin A. M., Holmes I. H. Derivation of neutralizing monoclonal antibodies against rotavirus. J Virol. 1983 Mar;45(3):1143–1146. doi: 10.1128/jvi.45.3.1143-1146.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thouless M. E., Beards G. M., Flewett T. H. Serotyping and subgrouping of rotavirus strains by the ELISA test. Arch Virol. 1982;73(3-4):219–230. doi: 10.1007/BF01318076. [DOI] [PubMed] [Google Scholar]
  16. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Bridger J. C., Snodgrass D. R., Herring J. A. Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Arch Virol. 1977;53(4):287–294. doi: 10.1007/BF01315627. [DOI] [PubMed] [Google Scholar]
  17. Woode G. N., Bridger J. C., Jones J. M., Flewett T. H., Davies H. A., Davis H. A., White G. B. Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals. Infect Immun. 1976 Sep;14(3):804–810. doi: 10.1128/iai.14.3.804-810.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wyatt R. G., Greenberg H. B., James W. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. Z. Definition of human rotavirus serotypes by plaque reduction assay. Infect Immun. 1982 Jul;37(1):110–115. doi: 10.1128/iai.37.1.110-115.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yewdell J. W., Gerhard W. Antigenic characterization of viruses by monoclonal antibodies. Annu Rev Microbiol. 1981;35:185–206. doi: 10.1146/annurev.mi.35.100181.001153. [DOI] [PubMed] [Google Scholar]
  20. Zissis G., Lambert J. P., Kapsenberg J. G., Enders G., Mutanda L. N. Human rotavirus serotypes. Lancet. 1981 Apr 25;1(8226):944–945. doi: 10.1016/s0140-6736(81)91639-1. [DOI] [PubMed] [Google Scholar]

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