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. 1984 Jul;51(1):147–153. doi: 10.1128/jvi.51.1.147-153.1984

Characterization of a Rous sarcoma virus mutant defective in packaging its own genomic RNA: biological properties of mutant TK15 and mutant-induced transformants.

S Kawai, T Koyama
PMCID: PMC254412  PMID: 6328034

Abstract

A mutant derived from a temperature-sensitive mutant of Rous sarcoma virus ( tsNY68 ) which showed extremely low infectivity was characterized. Infection of chicken embryo fibroblast cells with the mutant, TK15 , induced two types of transformants, mutant-producing 15c (+) and nonvirus -producing 15c (-) transformants. 15c (+) cells expressed all four viral genes normally and produced a normal level of virus particles. No complementation was observed between the mutant and avian leukosis viruses. However, when 15c (+) cells were cocultured with nonvirus -producing cells transformed by Y73, a replication-defective avian sarcoma virus, a high titer of Y73 virus was recovered. From its biological properties, the mutant seemed to have a defect(s) outside the viral genes. Biochemical analysis of the TK15 mutant (T. Koyama , F. Harada, and S. Kawai , J. Virol. 51:154-162, 1984) revealed that it had a defect in packaging its own genomic RNA. During replication of TK15 virus, the TK15 mutant appeared to segregate at high frequency more defective variants that induced 15c (-) transformants, in most of which only the src gene was expressed. The mechanism for the segregation of 15c (-) transformants is discussed with respect to the defect of the mutant.

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Selected References

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  1. Bishop J. M. Retroviruses. Annu Rev Biochem. 1978;47:35–88. doi: 10.1146/annurev.bi.47.070178.000343. [DOI] [PubMed] [Google Scholar]
  2. Gilboa E., Mitra S. W., Goff S., Baltimore D. A detailed model of reverse transcription and tests of crucial aspects. Cell. 1979 Sep;18(1):93–100. doi: 10.1016/0092-8674(79)90357-x. [DOI] [PubMed] [Google Scholar]
  3. Haseltine W. A., Maxam A. M., Gilbert W. Rous sarcoma virus genome is terminally redundant: the 5' sequence. Proc Natl Acad Sci U S A. 1977 Mar;74(3):989–993. doi: 10.1073/pnas.74.3.989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hayward W. S., Neel B. G. Retroviral gene expression. Curr Top Microbiol Immunol. 1981;91:217–276. doi: 10.1007/978-3-642-68058-8_8. [DOI] [PubMed] [Google Scholar]
  5. Kawai S., Duesberg P. H., Hanafusa H. Transformation-defective mutants of Rous sarcoma virus with src gene deletions of varying length. J Virol. 1977 Dec;24(3):910–914. doi: 10.1128/jvi.24.3.910-914.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kawai S., Hanafusa H. Genetic recombination with avian tumor virus. Virology. 1972 Jul;49(1):37–44. doi: 10.1016/s0042-6822(72)80005-9. [DOI] [PubMed] [Google Scholar]
  7. Kawai S., Hanafusa H. Isolation of defective mutant of avian sarcoma virus. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3493–3497. doi: 10.1073/pnas.70.12.3493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kawai S., Hanafusa H. Plaque assay for some strains of avian leukosis virus. Virology. 1972 Apr;48(1):126–135. doi: 10.1016/0042-6822(72)90120-1. [DOI] [PubMed] [Google Scholar]
  9. Kawai S., Hanafusa H. Recombination between a temperature-sensitive mutant and a deletion mutant of Rous sarcoma virus. J Virol. 1976 Aug;19(2):389–397. doi: 10.1128/jvi.19.2.389-397.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kawai S., Hanafusa H. The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant. Virology. 1971 Nov;46(2):470–479. doi: 10.1016/0042-6822(71)90047-x. [DOI] [PubMed] [Google Scholar]
  11. Kawai S., Metroka C. E., Hanafusa H. Complementation of functions required for cell transformation by double infection with RSV mutants. Virology. 1972 Jul;49(1):302–304. doi: 10.1016/s0042-6822(72)80032-1. [DOI] [PubMed] [Google Scholar]
  12. Kawai S. Transformation of rat cells by fusion-infection with Rous sarcoma virus. J Virol. 1980 Jun;34(3):772–776. doi: 10.1128/jvi.34.3.772-776.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kawai S., Yamamoto T. Isolation of different kinds of non-virus producing chick cells transformed by Schmidt-Ruppin strain (subgroup A) of Rous sarcoma virus. Jpn J Exp Med. 1970 Aug;40(4):243–256. [PubMed] [Google Scholar]
  14. Kawai S., Yoshida M., Segawa K., Sugiyama H., Ishizaki R., Toyoshima K. Characterization of Y73, an avian sarcoma virus: a unique transforming gene and its product, a phosphopolyprotein with protein kinase activity. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6199–6203. doi: 10.1073/pnas.77.10.6199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Koyama T., Harada F., Kawai S. Characterization of a Rous sarcoma virus mutant defective in packaging its own genomic RNA: biochemical properties of mutant TK15 and mutant-induced transformants. J Virol. 1984 Jul;51(1):154–162. doi: 10.1128/jvi.51.1.154-162.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Linial M., Medeiros E., Hayward W. S. An avian oncovirus mutant (SE 21Q1b) deficient in genomic RNA: biological and biochemical characterization. Cell. 1978 Dec;15(4):1371–1381. doi: 10.1016/0092-8674(78)90062-4. [DOI] [PubMed] [Google Scholar]
  17. Linial M. Transfer of defective avian tumor virus genomes by a Rous sarcoma virus RNA packaging mutant. J Virol. 1981 Apr;38(1):380–382. doi: 10.1128/jvi.38.1.380-382.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mann R., Mulligan R. C., Baltimore D. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell. 1983 May;33(1):153–159. doi: 10.1016/0092-8674(83)90344-6. [DOI] [PubMed] [Google Scholar]
  19. Rubin H. A VIRUS IN CHICK EMBRYOS WHICH INDUCES RESISTANCE IN VITRO TO INFECTION WITH ROUS SARCOMA VIRUS. Proc Natl Acad Sci U S A. 1960 Aug;46(8):1105–1119. doi: 10.1073/pnas.46.8.1105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shank P. R., Linial M. Avian oncovirus mutant (SE21Q1b) deficient in genomic RNA: characterization of a deletion in the provirus. J Virol. 1980 Nov;36(2):450–456. doi: 10.1128/jvi.36.2.450-456.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sorge J., Ricci W., Hughes S. H. cis-Acting RNA packaging locus in the 115-nucleotide direct repeat of Rous sarcoma virus. J Virol. 1983 Dec;48(3):667–675. doi: 10.1128/jvi.48.3.667-675.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stacey D. W. Expression of a subgenomic retroviral messenger RNA. Cell. 1980 Oct;21(3):811–820. doi: 10.1016/0092-8674(80)90444-4. [DOI] [PubMed] [Google Scholar]
  23. Stacey D. W. Messenger activity of virion RNA for avian leukosis viral envelope glycoprotein. J Virol. 1979 Mar;29(3):949–956. doi: 10.1128/jvi.29.3.949-956.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Taylor J. M. DNA intermediates of avian RNA tumor viruses. Curr Top Microbiol Immunol. 1979;87:23–41. doi: 10.1007/978-3-642-67344-3_2. [DOI] [PubMed] [Google Scholar]
  25. Varmus H. E. Form and function of retroviral proviruses. Science. 1982 May 21;216(4548):812–820. doi: 10.1126/science.6177038. [DOI] [PubMed] [Google Scholar]
  26. Vogt P. K. Genetically stable reassortment of markers during mixed infection with avian tumor viruses. Virology. 1971 Dec;46(3):947–952. doi: 10.1016/0042-6822(71)90093-6. [DOI] [PubMed] [Google Scholar]
  27. Watanabe S., Temin H. M. Encapsidation sequences for spleen necrosis virus, an avian retrovirus, are between the 5' long terminal repeat and the start of the gag gene. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5986–5990. doi: 10.1073/pnas.79.19.5986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Weiss R. A., Mason W. S., Vogt P. K. Genetic recombinants and heterozygotes derived from endogenous and exogenous avian RNA tumor viruses. Virology. 1973 Apr;52(2):535–552. doi: 10.1016/0042-6822(73)90349-8. [DOI] [PubMed] [Google Scholar]
  29. Yoshida M., Kawai S., Toyoshima K. Genome structure of avian sarcoma virus Y73 and unique sequence coding for polyprotein p90. J Virol. 1981 May;38(2):430–437. doi: 10.1128/jvi.38.2.430-437.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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