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. 1984 Jul;51(1):215–222. doi: 10.1128/jvi.51.1.215-222.1984

mRNA discrimination in extracts from uninfected and reovirus-infected L-cells.

R Lemieux, H Zarbl, S Millward
PMCID: PMC254420  PMID: 6328041

Abstract

Uncapped reovirus mRNA extracted at late times from infected L-cells is preferentially translated in extracts from infected L-cells. However, translation of this uncapped, late, reovirus mRNA in extracts from infected cells is sensitive to inhibition by the cap analog m7GTP . These results imply that reovirus infection does not induce a transition from cap-dependent to cap-independent translation. Nevertheless, the results of in vitro translational competition experiments between L-cell mRNA and late viral mRNA were consistent with the view that reovirus does induce an alteration in the cap-dependent translational apparatus of L-cells. The reduced efficiency of translation of a variety of capped mRNAs in extracts from infected cells is also consistent with this notion. We further conclude that a factor exists in reovirus-infected L-cells that specifically stimulates translation of uncapped reovirus mRNAs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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