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. 1984 Jul;51(1):242–246. doi: 10.1128/jvi.51.1.242-246.1984

mlt Mutation in the polyomavirus genome impairing a function of the middle T protein.

C Gelinas, S Masse, M Bastin
PMCID: PMC254424  PMID: 6328044

Abstract

The DNA from polyomavirus mlt mutant P155 transforms cells in culture as efficiently as wild-type DNA but has a much lower tumorigenic potential when injected into newborn rodents. The mutant has a 12-base-pair deletion between nucleotides 1347 and 1360, i.e., in a region which encodes parts of the middle and large T antigens (G elinas et al., J. Virol. 43:1072-1081, 1982). To determine which of the two viral gene functions was affected by the mutation, we transferred the latter into a modified polyomavirus genome encoding exclusively the middle T protein. Our results show that the P155 mutation alters a function of the polyomavirus middle T protein required for the induction of the tumorigenic process in vivo. Beside the 12-base-pair deletion at 96.3 map units, there is no other alteration in the coding sequence of P155 middle T with respect to that of P16, the wild-type parental strain. We conclude, therefore, that the deletion is the lesion affecting the tumorigenic potential of mutant P155 .

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asselin C., Gelinas C., Bastin M. Role of the three polyoma virus early proteins in tumorigenesis. Mol Cell Biol. 1983 Aug;3(8):1451–1459. doi: 10.1128/mcb.3.8.1451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Asselin C., Gélinas C., Branton P. E., Bastin M. Polyoma middle T antigen requires cooperation from another gene to express the malignant phenotype in vivo. Mol Cell Biol. 1984 Apr;4(4):755–760. doi: 10.1128/mcb.4.4.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benjamin T. L. The hr-t gene of polyoma virus. Biochim Biophys Acta. 1982 Dec 21;695(2):69–95. doi: 10.1016/0304-419x(82)90018-x. [DOI] [PubMed] [Google Scholar]
  4. Crick F. H. Codon--anticodon pairing: the wobble hypothesis. J Mol Biol. 1966 Aug;19(2):548–555. doi: 10.1016/s0022-2836(66)80022-0. [DOI] [PubMed] [Google Scholar]
  5. DIAMOND L., CRAWFORD L. V. SOME CHARACTERISTICS OF LARGE-PLAQUE AND SMALL-PLAQUE LINES OF POLYOMA VIRUS. Virology. 1964 Feb;22:235–244. doi: 10.1016/0042-6822(64)90008-x. [DOI] [PubMed] [Google Scholar]
  6. Eckhart W. Complementation and transformation by temperature-sensitive mutants of polyoma virus. Virology. 1969 May;38(1):120–125. doi: 10.1016/0042-6822(69)90133-0. [DOI] [PubMed] [Google Scholar]
  7. Flanagan S. P. 'Nude', a new hairless gene with pleiotropic effects in the mouse. Genet Res. 1966 Dec;8(3):295–309. doi: 10.1017/s0016672300010168. [DOI] [PubMed] [Google Scholar]
  8. Gelinas C., Chartrand P., Bastin M. Polyoma virus mutant with normal transforming ability but impaired tumorigenic potential. J Virol. 1982 Sep;43(3):1072–1081. doi: 10.1128/jvi.43.3.1072-1081.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Griffin B. E., Dilworth S. M. Polyomavirus: an overview of its unique properties. Adv Cancer Res. 1983;39:183–268. doi: 10.1016/s0065-230x(08)61036-2. [DOI] [PubMed] [Google Scholar]
  10. Griffin B. E., Maddock C. New classes of viable deletion mutants in the early region of polyoma virus. J Virol. 1979 Sep;31(3):645–656. doi: 10.1128/jvi.31.3.645-656.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lewis A. M., Jr, Cook J. L. Presence of allograft-rejection resistance in simian virus 40-transformed hamster cells and its possible role in tumor development. Proc Natl Acad Sci U S A. 1980 May;77(5):2886–2889. doi: 10.1073/pnas.77.5.2886. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCance D. J. Growth and persistence of polyoma early region deletion mutants in mice. J Virol. 1981 Sep;39(3):958–962. doi: 10.1128/jvi.39.3.958-962.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pantelouris E. M. Absence of thymus in a mouse mutant. Nature. 1968 Jan 27;217(5126):370–371. doi: 10.1038/217370a0. [DOI] [PubMed] [Google Scholar]
  15. Rassoulzadegan M., Cowie A., Carr A., Glaichenhaus N., Kamen R., Cuzin F. The roles of individual polyoma virus early proteins in oncogenic transformation. Nature. 1982 Dec 23;300(5894):713–718. doi: 10.1038/300713a0. [DOI] [PubMed] [Google Scholar]
  16. Rassoulzadegan M., Naghashfar Z., Cowie A., Carr A., Grisoni M., Kamen R., Cuzin F. Expression of the large T protein of polyoma virus promotes the establishment in culture of "normal" rodent fibroblast cell lines. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4354–4358. doi: 10.1073/pnas.80.14.4354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rothwell V. M., Folk W. R. Comparison of the DNA sequence of the Crawford small-plaque variant of polyomavirus with those of polyomaviruses A2 and strain 3. J Virol. 1983 Nov;48(2):472–480. doi: 10.1128/jvi.48.2.472-480.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Seif R., Cuzin F. Temperature-sensitive growth regulation in one type of transformed rat cells induced by the tsa mutant of polyoma virus. J Virol. 1977 Dec;24(3):721–728. doi: 10.1128/jvi.24.3.721-728.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Soeda E., Arrand J. R., Smolar N., Griffin B. E. Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigens. Cell. 1979 Jun;17(2):357–370. doi: 10.1016/0092-8674(79)90162-4. [DOI] [PubMed] [Google Scholar]
  20. Soeda E., Arrand J. R., Smolar N., Walsh J. E., Griffin B. E. Coding potential and regulatory signals of the polyoma virus genome. Nature. 1980 Jan 31;283(5746):445–453. doi: 10.1038/283445a0. [DOI] [PubMed] [Google Scholar]
  21. Treisman R., Novak U., Favaloro J., Kamen R. Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein. Nature. 1981 Aug 13;292(5824):595–600. doi: 10.1038/292595a0. [DOI] [PubMed] [Google Scholar]

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