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Journal of Virology logoLink to Journal of Virology
. 1984 Aug;51(2):452–457. doi: 10.1128/jvi.51.2.452-457.1984

Immediate-early transcription of Herpesvirus saimiri.

W Bodemer, E Knust, S Angermüller, B Fleckenstein
PMCID: PMC254459  PMID: 6086956

Abstract

Transcription of Herpesvirus saimiri was characterized during the initial phases of productive infection by Northern blot analyses and hybridizations of radioactive cDNA with cloned fragments of virion L-DNA. Under conditions of immediate-early transcription, e.g., blocking of viral protein synthesis by cycloheximide, a single cytoplasmic polyadenylated viral RNA of 2.7 kilobases was found in infected cells. The sequence coding for this RNA was between map units 0.89 and 0.93; it was transcribed from right to left in prototype arrangement of M-DNA. The immediate-early mRNA of lytically infected cells appeared to be very similar, if not identical, to the single viral RNA species found in lymphoid cells transformed by H. saimiri.

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Selected References

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