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. 1984 Aug;51(2):489–496. doi: 10.1128/jvi.51.2.489-496.1984

Identification of a bovine rotavirus gene and gene product influencing cellular attachment.

M Sabara, L A Babiuk
PMCID: PMC254464  PMID: 6086958

Abstract

Preliminary characterization of naturally occurring temperature-sensitive bovine rotavirus mutants, generated via gene reassortment, indicated that the product of gene segment 10 can influence virus adsorption to MA-104 cells. Cell-free translation of this gene segment in a rabbit reticulocyte system was then utilized to identify a polypeptide with a molecular weight of ca. 21,500 as the primary translation product. This identification provides a basis for further studies of rotavirus assembly and cell interactions which may, in turn, aid in elucidating the replicative strategy of the virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babiuk L. A., Mohammed K., Spence L., Fauvel M., Petro R. Rotavirus isolation and cultivation in the presence of trypsin. J Clin Microbiol. 1977 Dec;6(6):610–617. doi: 10.1128/jcm.6.6.610-617.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet. 1974 Feb 2;1(7849):149–151. doi: 10.1016/s0140-6736(74)92440-4. [DOI] [PubMed] [Google Scholar]
  3. Bridger J. C. Location of type-specific antigens in calf rotaviruses. J Clin Microbiol. 1978 Dec;8(6):625–628. doi: 10.1128/jcm.8.6.625-628.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carpio M. M., Babiuk L. A., Misra V., Blumenthal R. M. Bovine rotavirus-cell interactions: effect of virus infection on cellular integrity and macromolecular synthesis. Virology. 1981 Oct 15;114(1):86–97. doi: 10.1016/0042-6822(81)90255-5. [DOI] [PubMed] [Google Scholar]
  5. Clark S. M., Roth J. R., Clark M. L., Barnett B. B., Spendlove R. S. Trypsin enhancement of rotavirus infectivity: mechanism of enhancement. J Virol. 1981 Sep;39(3):816–822. doi: 10.1128/jvi.39.3.816-822.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  7. Cohen J. Ribonucleic acid polymerase activity associated with purified calf rotavirus. J Gen Virol. 1977 Sep;36(3):395–402. doi: 10.1099/0022-1317-36-3-395. [DOI] [PubMed] [Google Scholar]
  8. Elleman T. C., Hoyne P. A., Dyall-Smith M. L., Holmes I. H., Azad A. A. Nucleotide sequence of the gene encoding the serotype-specific glycoprotein of UK bovine rotavirus. Nucleic Acids Res. 1983 Jul 25;11(14):4689–4701. doi: 10.1093/nar/11.14.4689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Espejo R. T., Calderón E., González N., Salomon A., Martuscelli A., Romero P. Presence of two distinct types of rotavirus in infants and young children hospitalized with acute gastroenteritis in Mexico City, 1977. J Infect Dis. 1979 Apr;139(4):474–477. doi: 10.1093/infdis/139.4.474. [DOI] [PubMed] [Google Scholar]
  10. Estes M. K., Graham D. Y., Ramig R. F., Ericson B. L. Heterogeneity in the structural glycoprotein (VP7) of simian rotavirus SA11. Virology. 1982 Oct 15;122(1):8–14. doi: 10.1016/0042-6822(82)90372-5. [DOI] [PubMed] [Google Scholar]
  11. Fauvel M., Spence L., Babiuk L. A., Petro R., Bloch S. Hemagglutination and hemagglutination-inhibition studies with a strain of Nebraska calf diarrhea virus (bovine rotavirus). Intervirology. 1978;9(2):95–105. doi: 10.1159/000148927. [DOI] [PubMed] [Google Scholar]
  12. Gorziglia M., Esparza J. Poly(A) polymerase activity in human rotavirus. J Gen Virol. 1981 Apr;53(Pt 2):357–362. doi: 10.1099/0022-1317-53-2-357. [DOI] [PubMed] [Google Scholar]
  13. Graham D. Y., Estes M. K. Proteolytic enhancement of rotavirus infectivity: biology mechanism. Virology. 1980 Mar;101(2):432–439. doi: 10.1016/0042-6822(80)90456-0. [DOI] [PubMed] [Google Scholar]
  14. Greenberg H. B., Flores J., Kalica A. R., Wyatt R. G., Jones R. Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. J Gen Virol. 1983 Feb;64(Pt 2):313–320. doi: 10.1099/0022-1317-64-2-313. [DOI] [PubMed] [Google Scholar]
  15. Greenberg H. B., Kalica A. R., Wyatt R. G., Jones R. W., Kapikian A. Z., Chanock R. M. Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus. Proc Natl Acad Sci U S A. 1981 Jan;78(1):420–424. doi: 10.1073/pnas.78.1.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Greenberg H. B., Valdesuso J., van Wyke K., Midthun K., Walsh M., McAuliffe V., Wyatt R. G., Kalica A. R., Flores J., Hoshino Y. Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. J Virol. 1983 Aug;47(2):267–275. doi: 10.1128/jvi.47.2.267-275.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hruska J. F., Notter M. F., Menegus M. A., Steinhoff M. C. RNA polymerase associated with human rotaviruses in diarrhea stools. J Virol. 1978 May;26(2):544–546. doi: 10.1128/jvi.26.2.544-546.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kalica A. R., Flores J., Greenberg H. B. Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation. Virology. 1983 Feb;125(1):194–205. doi: 10.1016/0042-6822(83)90073-9. [DOI] [PubMed] [Google Scholar]
  19. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology. 1981 Jul 30;112(2):385–390. doi: 10.1016/0042-6822(81)90285-3. [DOI] [PubMed] [Google Scholar]
  20. Kapikian A. Z., Cline W. L., Greenberg H. B., Wyatt R. G., Kalica A. R., Banks C. E., James H. D., Jr, Flores J., Chanock R. M. Antigenic characterization of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA): evidence for distinctness of IAHA and neutralization antigens. Infect Immun. 1981 Aug;33(2):415–425. doi: 10.1128/iai.33.2.415-425.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  22. Mason B. B., Graham D. Y., Estes M. K. Biochemical mapping of the simian rotavirus SA11 genome. J Virol. 1983 May;46(2):413–423. doi: 10.1128/jvi.46.2.413-423.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Matsuno S., Hasegawa A., Kalica A. R., Kono R. Isolation of a recombinant between simian and bovine rotaviruses. J Gen Virol. 1980 May;48(1):253–256. doi: 10.1099/0022-1317-48-1-253. [DOI] [PubMed] [Google Scholar]
  24. McCrae M. A., Joklik W. K. The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3. Virology. 1978 Sep;89(2):578–593. doi: 10.1016/0042-6822(78)90199-x. [DOI] [PubMed] [Google Scholar]
  25. McCrae M. A., McCorquodale J. G. The molecular biology of rotaviruses. II. Identification of the protein-coding assignments of calf rotavirus genome RNA species. Virology. 1982 Mar;117(2):435–443. doi: 10.1016/0042-6822(82)90482-2. [DOI] [PubMed] [Google Scholar]
  26. Mebus C. A., Wyatt R. G., Kapikian A. Z. Intestinal lesions induced in gnotobiotic calves by the virus of human infantile gastroenteritis. Vet Pathol. 1977 May;14(3):273–282. doi: 10.1177/030098587701400310. [DOI] [PubMed] [Google Scholar]
  27. Pelham H. R., Jackson R. J. An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976 Aug 1;67(1):247–256. doi: 10.1111/j.1432-1033.1976.tb10656.x. [DOI] [PubMed] [Google Scholar]
  28. Petri T., Patterson S., Dimmock N. J. Polymorphism of the NS1 proteins of type A influenza virus. J Gen Virol. 1982 Aug;61(Pt 2):217–231. doi: 10.1099/0022-1317-61-2-217. [DOI] [PubMed] [Google Scholar]
  29. Petrie B. L., Estes M. K., Graham D. Y. Effects of tunicamycin on rotavirus morphogenesis and infectivity. J Virol. 1983 Apr;46(1):270–274. doi: 10.1128/jvi.46.1.270-274.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sabara M., Babiuk L. A., Gilchrist J., Misra V. Effect of tunicamycin on rotavirus assembly and infectivity. J Virol. 1982 Sep;43(3):1082–1090. doi: 10.1128/jvi.43.3.1082-1090.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sabara M., Deregt D., Babiuk L. A., Misra V. Genetic heterogeneity within individual bovine rotavirus isolates. J Virol. 1982 Dec;44(3):813–822. doi: 10.1128/jvi.44.3.813-822.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  33. Smith E. M., Estes M. K., Graham D. Y., Gerba C. P. A plaque assay for the simian rotavirus SAII. J Gen Virol. 1979 Jun;43(3):513–519. doi: 10.1099/0022-1317-43-3-513. [DOI] [PubMed] [Google Scholar]
  34. Spence L., Fauvel M., Petro R., Babiuk L. A. Comparison of rotavirus strains by hemagglutination inhibition. Can J Microbiol. 1978 Apr;24(4):353–362. doi: 10.1139/m78-059. [DOI] [PubMed] [Google Scholar]
  35. Weiner H. L., Drayna D., Averill D. R., Jr, Fields B. N. Molecular basis of reovirus virulence: role of the S1 gene. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5744–5748. doi: 10.1073/pnas.74.12.5744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Weiner H. L., Fields B. N. Neutralization of reovirus: the gene responsible for the neutralization antigen. J Exp Med. 1977 Nov 1;146(5):1305–1310. doi: 10.1084/jem.146.5.1305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Weiner H. L., Ramig R. F., Mustoe T. A., Fields B. N. Identification of the gene coding for the hemagglutinin of reovirus. Virology. 1978 May 15;86(2):581–584. doi: 10.1016/0042-6822(78)90099-5. [DOI] [PubMed] [Google Scholar]
  38. Woode G. N., Bridger J. C., Hall G., Dennis M. J. The isolation of a reovirus-like agent associated with diarrhoea in colostrum-deprived calves in Great Britain. Res Vet Sci. 1974 Jan;16(1):102–105. [PubMed] [Google Scholar]

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