Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1984 Aug;51(2):553–555. doi: 10.1128/jvi.51.2.553-555.1984

A bacteriophage T5 mutant with an increased frequency of single-chain interruptions.

M Rhoades
PMCID: PMC254472  PMID: 6086964

Abstract

A T5 mutant whose DNA has two sites where single-chain interruptions occur with a higher frequency than in wild-type DNA was isolated. Both sites occurred within the same strand as do the natural interruptions in T5 DNA, and both were due to alteration of the mechanism that generates the interruptions.

Full text

PDF
553

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carter D. M., Radding C. M. The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease. J Biol Chem. 1971 Apr 25;246(8):2502–2512. [PubMed] [Google Scholar]
  2. Everett R. D. DNA replication of bacteriophage T5. 3. Studies on the structure of concatemeric T5 DNA. J Gen Virol. 1981 Jan;52(Pt 1):25–38. doi: 10.1099/0022-1317-52-1-25. [DOI] [PubMed] [Google Scholar]
  3. Hayward G. S., Smith M. G. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972 Feb 14;63(3):383–395. doi: 10.1016/0022-2836(72)90435-4. [DOI] [PubMed] [Google Scholar]
  4. Masamune Y., Fleischman R. A., Richardson C. C. Enzymatic removal and replacement of nucleotides at single strand breaks in deoxyribonucleic acid. J Biol Chem. 1971 Apr 25;246(8):2680–2691. [PubMed] [Google Scholar]
  5. Rhoades M. Localization of single-chain interruptions in bacteriophage T5 DNA. II. Electrophoretic studies. J Virol. 1977 Sep;23(3):737–750. doi: 10.1128/jvi.23.3.737-750.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Rhoades M. New physical map of bacteriophage T5 DNA. J Virol. 1982 Aug;43(2):566–573. doi: 10.1128/jvi.43.2.566-573.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rogers S. G., Godwin E. A., Shinosky E. S., Rhoades M. Interruption-deficient mutants of bacteriophage T5 I. Isolation and general properties. J Virol. 1979 Feb;29(2):716–725. doi: 10.1128/jvi.29.2.716-725.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rogers S. G., Rhoades M. Bacteriophage T5-induced endonucleases that introduce site-specific single-chain interruptions in duplex DNA. Proc Natl Acad Sci U S A. 1976 May;73(5):1576–1580. doi: 10.1073/pnas.73.5.1576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Scheible P. P., Rhoades E. A., Rhoades M. Localization of single-chain interruptions in bacteriophage T5 DNA I. Electron microscopic studies. J Virol. 1977 Sep;23(3):725–736. doi: 10.1128/jvi.23.3.725-736.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES