Abstract
The nature of neutrophil-respiratory syncytial virus (RSV) interaction was investigated by assessing factors that influence neutrophil adherence to RSV-infected tissue culture monolayers. The adherence of neutrophils to infected cells was directly proportional to the degree of RSV replication as evidenced by infectious virus production, cytopathological changes, or viral antigen appearance. Sixty-one percent of the neutrophils adhered to the RSV-infected cells as compared with 52.7% on noninfected monolayers (P less than 0.05). The addition of RSV-specific antibody markedly increased polymorphonuclear leukocyte adherence to 88.5% (P less than 0.001). Complement in the absence of antibody augmented polymorphonuclear leukocyte adherence, but to a lesser degree, 69.0% (P less than 0.025). Arachidonic acid metabolism appeared to play a critical role in the adherence process; thromboxane was the single most important arachidonic acid metabolite. Inhibition of thromboxane synthesis reduced antibody-dependent polymorphonuclear leukocyte adherence on RSV-infected cells to 52.3% (P less than 0.025). These observations suggest a role for neutrophils in RSV infection. It is proposed that neutrophils may participate in RSV infection at the site of viral replication through the attachment to infected cells and the subsequent release of mediators of inflammation.
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- Cines D. B., Lyss A. P., Bina M., Corkey R., Kefalides N. A., Friedman H. M. Fc and C3 receptors induced by herpes simplex virus on cultured human endothelial cells. J Clin Invest. 1982 Jan;69(1):123–128. doi: 10.1172/JCI110422. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Douglas R. G., Jr, Alford R. H., Cate T. R., Couch R. B. The leukocyte response during viral respiratory illness in man. Ann Intern Med. 1966 Mar;64(3):521–530. doi: 10.7326/0003-4819-64-3-521. [DOI] [PubMed] [Google Scholar]
- Faden H., Kaul T. N., Ogra P. L. Activation of oxidative and arachidonic acid metabolism in neutrophils by respiratory syncytial virus antibody complexes: possible role in disease. J Infect Dis. 1983 Jul;148(1):110–116. doi: 10.1093/infdis/148.1.110. [DOI] [PubMed] [Google Scholar]
- Feigin R. D., Shackelford P. G. Value of repeat lumbar puncture in the differential diagnosis of meningitis. N Engl J Med. 1973 Sep 13;289(11):571–574. doi: 10.1056/NEJM197309132891108. [DOI] [PubMed] [Google Scholar]
- Fitzpatrick F. A., Stringfellow D. A. Virus and interferon effects on cellular prostaglandin biosynthesis. J Immunol. 1980 Jul;125(1):431–437. [PubMed] [Google Scholar]
- Ingerman-Wojenski C., Silver M. J., Smith J. B., Macarak E. Bovine endothelial cells in culture produce thromboxane as well as prostacyclin. J Clin Invest. 1981 May;67(5):1292–1296. doi: 10.1172/JCI110157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaul A., Scott R., Gallagher M., Scott M., Clement J., Ogra P. L. Respiratory syncytial virus infection. Rapid diagnosis in children by use of indirect immunofluorescence. Am J Dis Child. 1978 Nov;132(11):1088–1090. doi: 10.1001/archpedi.1978.02120360044006. [DOI] [PubMed] [Google Scholar]
- Kaul T. N., Faden H., Ogra P. L. Effect of respiratory syncytial virus and virus-antibody complexes on the oxidative metabolism of human neutrophils. Infect Immun. 1981 May;32(2):649–654. doi: 10.1128/iai.32.2.649-654.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaul T. N., Welliver R. C., Ogra P. L. Appearance of complement components and immunoglobulins on nasopharyngeal epithelial cells following naturally acquired infection with respiratory syncytial virus. J Med Virol. 1982;9(2):149–158. doi: 10.1002/jmv.1890090210. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaul T. N., Welliver R. C., Ogra P. L. Comparison of fluorescent-antibody, neutralizing-antibody, and complement-enhanced neutralizing-antibody assays for detection of serum antibody to respiratory syncytial virus. J Clin Microbiol. 1981 May;13(5):957–962. doi: 10.1128/jcm.13.5.957-962.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kelly J. P., Johnson M. C., Parker C. W. Effect of inhibitors of arachidonic acid metabolism on mitogenesis in human lymphocytes: possible role of thromboxanes and products of the lipoxygenase pathway. J Immunol. 1979 Apr;122(4):1563–1571. [PubMed] [Google Scholar]
- MacGregor R. R., Friedman H. M., Macarak E. J., Kefalides N. A. Virus infection of endothelial cells increases granulocyte adherence. J Clin Invest. 1980 Jun;65(6):1469–1477. doi: 10.1172/JCI109811. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacGregor R. R., Macarak E. J., Kefalides N. A. Comparative adherence of granulocytes to endothelial monolayers and nylon fiber. J Clin Invest. 1978 Mar;61(3):697–702. doi: 10.1172/JCI108981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McGillen J., Patterson R., Phair J. P. Adherence of polymorphonuclear leukocytes to nylon: modulation by prostacyclin (PGI2), corticosteroids, and complement activation. J Infect Dis. 1980 Mar;141(3):382–388. doi: 10.1093/infdis/141.3.382. [DOI] [PubMed] [Google Scholar]
- Sieber O. F., Wilska M. L., Riggin R. Elevated nitroblue tetrazolium dye reduction test response in acute viral respiratory disease. Pediatrics. 1976 Jul;58(1):122–124. [PubMed] [Google Scholar]
- Smith T. F., McIntosh K., Fishaut M., Henson P. M. Activation of complement by cells infected with respiratory syncytial virus. Infect Immun. 1981 Jul;33(1):43–48. doi: 10.1128/iai.33.1.43-48.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spagnuolo P. J., Ellner J. J., Hassid A., Dunn M. J. Thromboxane A2 mediates augmented polymorphonuclear leukocyte adhesiveness. J Clin Invest. 1980 Sep;66(3):406–414. doi: 10.1172/JCI109870. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stevens D. A., Ferrington R. A., Jordan G. W., Merigan T. C. Cellular events in zoster vesicles: relation to clinical course and immune parameters. J Infect Dis. 1975 May;131(5):509–515. doi: 10.1093/infdis/131.5.509. [DOI] [PubMed] [Google Scholar]