Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1984 Oct;52(1):290–292. doi: 10.1128/jvi.52.1.290-292.1984

Monoclonal antibodies to a monkeypox virus polypeptide determinant.

L F Roumillat, J L Patton, M L Davis
PMCID: PMC254519  PMID: 6207310

Abstract

Three monkeypox virus (MPV) antibody-secreting murine monoclones were characterized as being of the immunoglobulin G1 isotype, gave a 4+ reaction in the indirect fluorescent-antibody test, gave a positive reaction in the enzyme immunoassay, and did not neutralize MPV. These monoclonal antibodies were determined by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis transblot method to react to a 15,500-molecular-weight MPV polypeptide. This reactivity could not be removed by adsorption to a vaccinia virus-infected cell suspension. The three monoclonal antibodies were specific for MPV when tested against epidemiologically unrelated isolates of cowpox virus, variola virus, vaccinia virus, and MPV.

Full text

PDF
290

Images in this article

290Image
on p.290
291Image
on p.291

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon R. Chemically defined antiviral vaccines. Annu Rev Microbiol. 1980;34:593–618. doi: 10.1146/annurev.mi.34.100180.003113. [DOI] [PubMed] [Google Scholar]
  2. Buchman T. G., Roizman B., Nahmias A. J. Demonstration of exogenous genital reinfection with herpes simplex virus type 2 by restriction endonuclease fingerprinting of viral DNA. J Infect Dis. 1979 Sep;140(3):295–304. doi: 10.1093/infdis/140.3.295. [DOI] [PubMed] [Google Scholar]
  3. Esposito J. J., Obijeski J. F., Nakano J. H. The virion and soluble antigen proteins of variola, monkeypox, and vaccinia viruses. J Med Virol. 1977;1(2):95–110. doi: 10.1002/jmv.1890010203. [DOI] [PubMed] [Google Scholar]
  4. Harper L., Bedson H. S., Buchan A. Identification of orthopoxviruses by polyacrylamide gel electrophoresis of intracellular polypeptides. I. Four major groupings. Virology. 1979 Mar;93(2):435–444. doi: 10.1016/0042-6822(79)90247-2. [DOI] [PubMed] [Google Scholar]
  5. Heinz F. X., Berger R., Majdic O., Knapp W., Kunz C. Monoclonal antibodies to the structural glycoprotein of tick-borne encephalitis virus. Infect Immun. 1982 Sep;37(3):869–874. doi: 10.1128/iai.37.3.869-874.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kim K. S., Sapienza V. J., Chen C. M., Wisniewski K. Production and characterization of monoclonal antibodies specific for a glycosylated polypeptide of human cytomegalovirus. J Clin Microbiol. 1983 Aug;18(2):331–343. doi: 10.1128/jcm.18.2.331-343.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Köhler G., Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol. 1976 Jul;6(7):511–519. doi: 10.1002/eji.1830060713. [DOI] [PubMed] [Google Scholar]
  8. Pereira L., Klassen T., Baringer J. R. Type-common and type-specific monoclonal antibody to herpes simplex virus type 1. Infect Immun. 1980 Aug;29(2):724–732. doi: 10.1128/iai.29.2.724-732.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Roumillat L. F., Feorino P. M., Caplan D. D., Lukert P. D. Analysis and characterization of herpes simplex virus after its persistence in a lymphoblastoid cell line for 15 months. Infect Immun. 1980 Aug;29(2):671–677. doi: 10.1128/iai.29.2.671-677.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smith G. E., Summers M. D. Application of a novel radioimmunoassay to identify baculovirus structural proteins that share interspecies antigenic determinants. J Virol. 1981 Jul;39(1):125–137. doi: 10.1128/jvi.39.1.125-137.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith J. S., Sumner J. W., Roumillat L. F. Enzyme immunoassay for rabies antibody in hybridoma culture fluids and its application to differentiation of street and laboratory strains of rabies virus. J Clin Microbiol. 1984 Feb;19(2):267–272. doi: 10.1128/jcm.19.2.267-272.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tsang V. C., Peralta J. M., Simons A. R. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol. 1983;92:377–391. doi: 10.1016/0076-6879(83)92032-3. [DOI] [PubMed] [Google Scholar]
  13. Ziegler D. W., Hutchinson H. D., Koplan J. P., Nakano J. H. Detection by radioimmunoassay of antibodies in human smallpox patients and vaccinees. J Clin Microbiol. 1975 Mar;1(3):311–317. doi: 10.1128/jcm.1.3.311-317.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES