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. 1984 Nov;52(2):624–629. doi: 10.1128/jvi.52.2.624-629.1984

SMX-1 virus-induced inhibition of ecotropic and recombinant proviral sequence amplification in thymuses of AKR mice.

D L Buchhagen, E Stockert
PMCID: PMC254566  PMID: 6092684

Abstract

SMX-1 virus delays the appearance of spontaneous thymomas in AKR mice which have been inoculated as young adults by the intrathymic route. Analyses of high-molecular-weight thymus DNAs from SMX-1 virus-inoculated AKR mice indicated the absence of 3' recombinant proviral-cellular DNA junction fragments generated by EcoRI and PvuII digestion. An average of five recombinant proviral fragments were detected in DNAs from spontaneous thymomas that developed in medium-injected control mice. Preleukemic mice that amplify murine leukemia virus-related antigens on their thymocyte surface contained unintegrated proviruses in their thymus DNAs, and 2.3-kilobase EcoRI and 2.1-kilobase PvuII recombinant DNA fragments were detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buchhagen D. L. Specific integration of recombinant proviral sequences in ecotropic Gross virus-accelerated AKR thymomas. Virology. 1984 Apr 30;134(2):287–295. doi: 10.1016/0042-6822(84)90298-8. [DOI] [PubMed] [Google Scholar]
  2. Chattopadhyay S. K., Lander M. R., Rands E., Lowy D. R. Structure of endogenous murine leukemia virus DNA in mouse genomes. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5774–5778. doi: 10.1073/pnas.77.10.5774. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cloyd M. W., Hartley J. W., Rowe W. P. Lymphomagenicity of recombinant mink cell focus-inducing murine leukemia viruses. J Exp Med. 1980 Mar 1;151(3):542–552. doi: 10.1084/jem.151.3.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789–792. doi: 10.1073/pnas.74.2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Herr W., Gilbert W. Germ-line MuLV reintegrations in AKR/J mice. Nature. 1982 Apr 29;296(5860):865–868. doi: 10.1038/296865a0. [DOI] [PubMed] [Google Scholar]
  6. Herr W., Gilbert W. Somatically acquired recombinant murine leukemia proviruses in thymic leukemias of AKR/J mice. J Virol. 1983 Apr;46(1):70–82. doi: 10.1128/jvi.46.1.70-82.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herr W., Schwartz D., Gilbert W. Isolation and mapping of cDNA hybridization probes specific for ecotropic and nonecotropic murine leukemia proviruses. Virology. 1983 Feb;125(1):139–154. doi: 10.1016/0042-6822(83)90070-3. [DOI] [PubMed] [Google Scholar]
  8. Jenkins N. A., Copeland N. G., Taylor B. A., Lee B. K. Organization, distribution, and stability of endogenous ecotropic murine leukemia virus DNA sequences in chromosomes of Mus musculus. J Virol. 1982 Jul;43(1):26–36. doi: 10.1128/jvi.43.1.26-36.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kawashima K., Ikeda H., Hartley J. W., Stockert E., Rowe W. P., Old L. J. Changes in expression of murine leukemia virus antigens and production of xenotropic virus in the late preleukemic period in AKR mice. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4680–4684. doi: 10.1073/pnas.73.12.4680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Khan A. S., Rowe W. P., Martin M. A. Cloning of endogenous murine leukemia virus-related sequences from chromosomal DNA of BALB/c and AKR/J mice: identification of an env progenitor of AKR-247 mink cell focus-forming proviral DNA. J Virol. 1982 Nov;44(2):625–636. doi: 10.1128/jvi.44.2.625-636.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Donnell P. V., Stockert E., Obata Y., Old L. J. Leukemogenic properties of AKR dualtropic (MCF) viruses: amplification of murine leukemia virus-related antigens on thymocytes and acceleration of leukemia development in AKR mice. Virology. 1981 Jul 30;112(2):548–563. doi: 10.1016/0042-6822(81)90301-9. [DOI] [PubMed] [Google Scholar]
  12. Rein A. Interference grouping of murine leukemia viruses: a distinct receptor for the MCF-recombinant viruses in mouse cells. Virology. 1982 Jul 15;120(1):251–257. doi: 10.1016/0042-6822(82)90024-1. [DOI] [PubMed] [Google Scholar]
  13. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  14. Steffen D. L., Taylor B. A., Weinberg R. A. Continuing germ line integration of AKV proviruses during the breeding of AKR mice and derivative recombinant inbred strains. J Virol. 1982 Apr;42(1):165–175. doi: 10.1128/jvi.42.1.165-175.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Steffen D., Bird S., Rowe W. P., Weinberg R. A. Identification of DNA fragments carrying ecotropic proviruses of AKR mice. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4554–4558. doi: 10.1073/pnas.76.9.4554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stockert E., O'Donnell P. V., Obata Y., Old L. J. Inhibition of AKR leukemogenesis by SMX-1, a dualtropic murine leukemia virus. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3720–3724. doi: 10.1073/pnas.77.6.3720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yoshimura F. K., Breda M. Lack of AKR ecotropic provirus amplification in AKR leukemic thymuses. J Virol. 1981 Sep;39(3):808–815. doi: 10.1128/jvi.39.3.808-815.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yoshimura F. K., Levine K. L. AKR thymic lymphomas involving mink cell focus-inducing murine leukemia viruses have a common region of provirus integration. J Virol. 1983 Feb;45(2):576–584. doi: 10.1128/jvi.45.2.576-584.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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