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. 1984 Nov;52(2):650–655. doi: 10.1128/jvi.52.2.650-655.1984

Induction of antibody to foot-and-mouth disease virus in presensitized mouse spleen cell cultures.

T Collen, K C McCullough, T R Doel
PMCID: PMC254569  PMID: 6092687

Abstract

Cultures of spleen cells from immunized mice were stimulated in vitro by soluble preparations of purified foot-and-mouth disease virus. Virus-specific antibody, as detected by an enzyme-linked immunosorbent assay, was produced by immune spleen cells but not by normal, nonimmune cells. The optimal specific response was obtained with 1 microgram of virus per ml of culture; as the virus concentration was increased, the production of specific antibody was reduced. For very low concentrations of virus (less than 0.01 microgram per culture), there was tentative evidence of suppression of the specific antibody response. The levels of specific antibody induced were dependent on the source and number of plastic-adherent cells present in the cultures. We intend to use this model system to study further the basis of immunity to foot-and-mouth disease virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abu Elzein E. M., Crowther J. R. Detection and quantification of IgM, IgA, IgG1 and IgG2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay. J Hyg (Lond) 1981 Feb;86(1):79–85. doi: 10.1017/s0022172400068765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abu Elzein E. M., Crowther J. R. Differentiation of foot-and-mouth disease virus strains using a competition enzyme-linked immunosorbent assay. J Virol Methods. 1982 Jan;3(6):355–365. doi: 10.1016/0166-0934(82)90040-4. [DOI] [PubMed] [Google Scholar]
  3. BACHRACH H. L., TRAUTMAN R., BREESE S. S., Jr CHEMICAL PHYSICAL PROPERTIES OF VIRTUALLY PURE FOOT-AND-MOUTH DISEASE VIRUS. Am J Vet Res. 1964 Mar;25:333–342. [PubMed] [Google Scholar]
  4. Doel T. R., Baccarini P. J. Thermal stability of foot-and-mouth disease virus. Arch Virol. 1981;70(1):21–32. doi: 10.1007/BF01320790. [DOI] [PubMed] [Google Scholar]
  5. Dosch H. M., Gelfand E. W. In vitro induction and measurement of hemolytic plaque forming cells in man. J Immunol Methods. 1976;11(2):107–116. doi: 10.1016/0022-1759(76)90138-1. [DOI] [PubMed] [Google Scholar]
  6. Golding S. M., Hedger R. S., Talbot P. Radial immuno-diffusion and serum-neutralisation techniques for the assay of antibodies to swine vesicular disease. Res Vet Sci. 1976 Mar;20(2):142–147. [PubMed] [Google Scholar]
  7. Graves J. H., McKercher P. D., Callis J. J. Foot-and-mouth disease vaccine: influence of the vaccine virus subtype on neutralizing antibody and resistance to disease. Am J Vet Res. 1972 Apr;33(4):765–768. [PubMed] [Google Scholar]
  8. Heijnen C. J., UytdeHaag F., Gmelig-Meyling F. H., Ballieux R. E. Localization of human antigen-specific helper and suppressor function in distinct T-cell subpopulations. Cell Immunol. 1979 Mar 15;43(2):282–292. doi: 10.1016/0008-8749(79)90173-4. [DOI] [PubMed] [Google Scholar]
  9. Kirchner H., Tosato G., Blaese R. M., Broder S., Magrath I. T. Polyclonal immunoglobulin secretion by human B lymphocytes exposed to Epstein-Barr virus in vitro. J Immunol. 1979 Apr;122(4):1310–1313. [PubMed] [Google Scholar]
  10. Knudsen R. C., Groocock C. M., Andersen A. A. Immunity to foot-and-mouth disease virus in guinea pigs: clinical and immune responses. Infect Immun. 1979 Jun;24(3):787–792. doi: 10.1128/iai.24.3.787-792.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Koprowski H., Mocarelli P., Wiktor T. J. Antibody response in vitro to an animal virus: production of rabies virus neutralizing antibodies by mouse cells in culture. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2433–2436. doi: 10.1073/pnas.69.9.2433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kraaijeveld C. A., la Rivière G., Benaissa-Trouw B. J., Jansen J., Harmsen T., Snippe H. Effect of the adjuvant dimethyl dioctadecyl ammonium bromide on the humoral and cellular immune responses to encephalomyocarditis virus. Antiviral Res. 1983 Sep;3(3):137–149. doi: 10.1016/0166-3542(83)90021-9. [DOI] [PubMed] [Google Scholar]
  13. Lamb J. R., Woody J. N., Hartzman R. J., Eckels D. D. In vitro influenza virus-specific antibody production in man: antigen-specific and HLA-restricted induction of helper activity mediated by cloned human T lymphocytes. J Immunol. 1982 Oct;129(4):1465–1470. [PubMed] [Google Scholar]
  14. Lane H. C., Volkman D. J., Whalen G., Fauci A. S. In vitro antigen-induced, antigen-specific antibody production in man. Specific and polyclonal components, kinetics, and cellular requirements. J Exp Med. 1981 Oct 1;154(4):1043–1057. doi: 10.1084/jem.154.4.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Luzzati A. L., Taussig M. J., Meo T., Pernis B. Induction of an antibody response in cultures of human peripheral blood lymphocytes. J Exp Med. 1976 Sep 1;144(3):573–585. doi: 10.1084/jem.144.3.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mishell R. I., Dutton R. W. Immunization of normal mouse spleen cell suspensions in vitro. Science. 1966 Aug 26;153(3739):1004–1006. doi: 10.1126/science.153.3739.1004. [DOI] [PubMed] [Google Scholar]
  17. Unanue E. R. The regulatory role of macrophages in antigenic stimulation. Part Two: symbiotic relationship between lymphocytes and macrophages. Adv Immunol. 1981;31:1–136. doi: 10.1016/s0065-2776(08)60919-0. [DOI] [PubMed] [Google Scholar]
  18. Uytdehaag F. G., Osterhaus A. D., Loggen H. G., Bakker R. H., van Asten J. A., Kreeftenberg J. G., van der Marel P., van Steenis B. Induction of antigen-specific antibody response in human peripheral blood lymphocytes in vitro by a dog kidney cell vaccine against rabies virus (DKCV). J Immunol. 1983 Sep;131(3):1234–1239. [PubMed] [Google Scholar]
  19. Wong C. Y., Woodruff J. J., Woodruff J. F. Generation of cytotoxic T lymphocytes during coxsackievirus tb-3 infection. II. Characterization of effector cells and demonstration cytotoxicity against viral-infected myofibers1. J Immunol. 1977 Apr;118(4):1165–1169. [PubMed] [Google Scholar]
  20. Yarchoan R., Murphy B. R., Strober W., Schneider H. S., Nelson D. L. Specific anti-influenza virus antibody production in vitro by human peripheral blood mononuclear cells. J Immunol. 1981 Dec;127(6):2588–2594. [PubMed] [Google Scholar]

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