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. 1984 Dec;52(3):892–896. doi: 10.1128/jvi.52.3.892-896.1984

Construction and properties of a ribosome-binding site mutation in gene E of phi X174 bacteriophage.

S Gillam, C R Astell, P Jahnke, C A Hutchison 3rd, M Smith
PMCID: PMC254610  PMID: 6238174

Abstract

Oligodeoxyribonucleotide mutagenesis has been used to produce a G----A mutation at nucleotide 557 of the phi X174 genome. This changes the ribosome-binding sequence GAGG of gene E to GAAG without affecting the amino acid, glutamine, encoded by the overlapping gene D. The phi X174rb(E)557 mutant does not lyse infected Escherichia coli C and therefore results in the accumulation of a large number intracellular mature phage particles. Thus, the mutation inactivates production of the gene E lytic product, presumably by blocking translation of gene E, without affecting other phage functions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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