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. 1984 Dec;52(3):1032–1035. doi: 10.1128/jvi.52.3.1032-1035.1984

Isolation of a polyomavirus with an insertion of foreign DNA in the early gene promoter region.

K L Clark, M M Bendig, W R Folk
PMCID: PMC254637  PMID: 6092703

Abstract

We have isolated a polyomavirus with 134 base pairs of foreign DNA between the origin of replication and the early promoter. The insertion reduces the infectivity of the virus by interfering with events required for the initiation of infection. mRNA transcripts from the early region exhibit a marked heterogeneity of 5' termini.

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Selected References

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  1. Bendig M. M., Folk W. R. Deletion mutants of polyoma virus defining a nonessential region between the origin of replication and the initiation codon for early proteins. J Virol. 1979 Nov;32(2):530–535. doi: 10.1128/jvi.32.2.530-535.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bendig M. M., Thomas T., Folk W. R. Regulatory mutants of polyoma virus defective in DNA replication and the synthesis of early proteins. Cell. 1980 Jun;20(2):401–409. doi: 10.1016/0092-8674(80)90626-1. [DOI] [PubMed] [Google Scholar]
  3. Bensimhon M., Gabarro-Arpa J., Ehrlich R., Reiss C. Physical characteristics in eucaryotic promoters. Nucleic Acids Res. 1983 Jul 11;11(13):4521–4540. doi: 10.1093/nar/11.13.4521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bolen J. B., Consigli R. A. Differential adsorption of polyoma virions and capsids to mouse kidney cells and guinea pig erythrocytes. J Virol. 1979 Nov;32(2):679–683. doi: 10.1128/jvi.32.2.679-683.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CRAWFORD L. V. The adsorption of polyoma virus. Virology. 1962 Oct;18:177–181. doi: 10.1016/0042-6822(62)90003-x. [DOI] [PubMed] [Google Scholar]
  6. Cowie A., Jat P., Kamen R. Determination of sequences at the capped 5' ends of polyoma virus early region transcripts synthesized in vivo and in vitro demonstrates an unusual microheterogeneity. J Mol Biol. 1982 Aug 5;159(2):225–255. doi: 10.1016/0022-2836(82)90494-6. [DOI] [PubMed] [Google Scholar]
  7. Deninger P. L., Esty A., LaPorte P., Hsu H., Friedmann T. The nucleotide sequence and restriction enzyme sites of the polyoma genome. Nucleic Acids Res. 1980 Feb 25;8(4):855–860. [PMC free article] [PubMed] [Google Scholar]
  8. Ding D., Jones M. D., Leigh-Brown A., Griffin B. E. Mutant din-21, a variant of polyoma virus containing a mouse DNA sequence in the viral genome. EMBO J. 1982;1(4):461–466. doi: 10.1002/j.1460-2075.1982.tb01191.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Favaloro J., Treisman R., Kamen R. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol. 1980;65(1):718–749. doi: 10.1016/s0076-6879(80)65070-8. [DOI] [PubMed] [Google Scholar]
  10. Fenton R. G., Basilico C. Changes in the topography of early region transcription during polyoma virus lytic infection. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7142–7146. doi: 10.1073/pnas.79.23.7142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Folk W. R., Fishel B. R. Tandem repetition of the origin of DNA replication in defective polyoma virus DNA's. Virology. 1975 Apr;64(2):447–453. doi: 10.1016/0042-6822(75)90122-1. [DOI] [PubMed] [Google Scholar]
  12. Folk W. R. Induction of virus synthesis in polyoma-transformed BHK-21 cells. J Virol. 1973 Mar;11(3):424–431. doi: 10.1128/jvi.11.3.424-431.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gaudray P., Tyndall C., Kamen R., Cuzin F. The high affinity binding site on polyoma virus DNA for the viral large-T protein. Nucleic Acids Res. 1981 Nov 11;9(21):5697–5710. doi: 10.1093/nar/9.21.5697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ghosh P. K., Lebowitz P. Simian virus 40 early mRNA's contain multiple 5' termini upstream and downstream from a Hogness-Goldberg sequence; a shift in 5' termini during the lytic cycle is mediated by large T antigen. J Virol. 1981 Oct;40(1):224–240. doi: 10.1128/jvi.40.1.224-240.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gluzman Y., Frisque R. J., Sambrook J. Origin-defective mutants of SV40. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):293–300. doi: 10.1101/sqb.1980.044.01.033. [DOI] [PubMed] [Google Scholar]
  16. Griffin B. E., Fried M. Amplification of a specific region of the polyoma virus genome. Nature. 1975 Jul 17;256(5514):175–179. doi: 10.1038/256175a0. [DOI] [PubMed] [Google Scholar]
  17. Hansen U., Tenen D. G., Livingston D. M., Sharp P. A. T antigen repression of SV40 early transcription from two promoters. Cell. 1981 Dec;27(3 Pt 2):603–613. doi: 10.1016/0092-8674(81)90402-5. [DOI] [PubMed] [Google Scholar]
  18. Heiser W. C., Eckhart W. Polyoma virus early and late mRNAs in productively infected mouse 3T6 cells. J Virol. 1982 Oct;44(1):175–188. doi: 10.1128/jvi.44.1.175-188.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  20. Howley P. M., Israel M. A., Law M. F., Martin M. A. A rapid method for detecting and mapping homology between heterologous DNAs. Evaluation of polyomavirus genomes. J Biol Chem. 1979 Jun 10;254(11):4876–4883. [PubMed] [Google Scholar]
  21. Jat P., Novak U., Cowie A., Tyndall C., Kamen R. DNA sequences required for specific and efficient initiation of transcription at the polyoma virus early promoter. Mol Cell Biol. 1982 Jul;2(7):737–751. doi: 10.1128/mcb.2.7.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Jat P., Roberts J. W., Cowie A., Kamen R. Comparison of the polyoma virus early and late promoters by transcription in vitro. Nucleic Acids Res. 1982 Feb 11;10(3):871–887. doi: 10.1093/nar/10.3.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kafatos F. C., Jones C. W., Efstratiadis A. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res. 1979 Nov 24;7(6):1541–1552. doi: 10.1093/nar/7.6.1541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Kamen R., Jat P., Treisman R., Favaloro J., Folk W. R. 5' termini of polyoma virus early region transcripts synthesized in vivo by wild-type virus and viable deletion mutants. J Mol Biol. 1982 Aug 5;159(2):189–224. doi: 10.1016/0022-2836(82)90493-4. [DOI] [PubMed] [Google Scholar]
  25. Katinka M., Yaniv M. Deletions of N-terminal sequences of polyoma virus T-antigens reduce but do not abolish transformation of rat fibroblasts. Mol Cell Biol. 1982 Oct;2(10):1238–1246. doi: 10.1128/mcb.2.10.1238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  27. Mackay R. L., Consigli R. A. Early events in polyoma virus infection: attachment, penetration, and nuclear entry. J Virol. 1976 Aug;19(2):620–636. doi: 10.1128/jvi.19.2.620-636.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  29. McKnight S. L., Kingsbury R. Transcriptional control signals of a eukaryotic protein-coding gene. Science. 1982 Jul 23;217(4557):316–324. doi: 10.1126/science.6283634. [DOI] [PubMed] [Google Scholar]
  30. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  31. Moreau P., Hen R., Wasylyk B., Everett R., Gaub M. P., Chambon P. The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants. Nucleic Acids Res. 1981 Nov 25;9(22):6047–6068. doi: 10.1093/nar/9.22.6047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Myers R. M., Tjian R. Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6491–6495. doi: 10.1073/pnas.77.11.6491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Pomerantz B. J., Mueller C. R., Hassell J. A. Polyomavirus large T antigen binds independently to multiple, unique regions on the viral genome. J Virol. 1983 Sep;47(3):600–610. doi: 10.1128/jvi.47.3.600-610.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Ricca G. A., Taylor J. M., Kalinyak J. E. Simple rapid method for the synthesis of radioactively labeled cDNA hybridization probes utilizing bacteriophage M13mp7. Proc Natl Acad Sci U S A. 1982 Feb;79(3):724–728. doi: 10.1073/pnas.79.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  36. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Shortle D., Nathans D. Regulatory mutants of simian virus 40: constructed mutants with base substitutions at the origin of DNA replication. J Mol Biol. 1979 Jul 15;131(4):801–817. doi: 10.1016/0022-2836(79)90202-x. [DOI] [PubMed] [Google Scholar]
  38. Türler H. Interaction of polyoma and mouse DNAs. IV. Time course and extent of integration of polyoma DNA into mouse DNA during lytic infection. J Virol. 1977 Aug;23(2):272–285. doi: 10.1128/jvi.23.2.272-285.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. de Villiers J., Schaffner W. A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs. Nucleic Acids Res. 1981 Dec 11;9(23):6251–6264. doi: 10.1093/nar/9.23.6251. [DOI] [PMC free article] [PubMed] [Google Scholar]

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