Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1985 Feb;53(2):345–349. doi: 10.1128/jvi.53.2.345-349.1985

Feline panleukopenia virus replicates in cells in which cellular DNA synthesis is blocked.

C Lenghaus, T K Mun, M J Studdert
PMCID: PMC254643  PMID: 2982022

Abstract

The components of the cell cycle for a feline embryo cell line were defined. Thymidine (6mM)-supplemented medium reversibly arrested cells 1 h into the S phase of the cell cycle and was used in a double blocking procedure to synchronize cells to the early S phase. The kinetics of feline panleukopenia virus replication in synchronized cells was studied by using (i) inclusion body formation, (ii) a plaque assay for cell-associated and cell-free virus under one-step growth conditions, (iii) an enzyme immunoassay for viral protein, (iv) electron microscopy of infected cells, and (v) the detection and identification of viral replicative form DNA by restriction endonuclease analysis. Parallel studies by each of these procedures of the replication of feline panleukopenia virus in cells in which a 6 mM thymidine block was maintained indicated that parvovirus replicated with essentially similar kinetics in both unblocked, synchronized cells and in cells in which the block was maintained. Accordingly, a 6 mM thymidine-supplemented medium, although it effectively blocks cellular DNA synthesis, does not block the replication of parvovirus.

Full text

PDF
345

Images in this article

348Image
on p.348

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Astell C. R., Thomson M., Chow M. B., Ward D. C. Structure and replication of minute virus of mice DNA. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):751–762. doi: 10.1101/sqb.1983.047.01.086. [DOI] [PubMed] [Google Scholar]
  2. Bernhard W. A new staining procedure for electron microscopical cytology. J Ultrastruct Res. 1969 May;27(3):250–265. doi: 10.1016/s0022-5320(69)80016-x. [DOI] [PubMed] [Google Scholar]
  3. Galavazi G., Schenk H., Bootsma D. Synchronization of mammalian cells in vitro by inhibition of the DNA synthesis. I. Optimal conditions. Exp Cell Res. 1966 Feb;41(2):428–437. doi: 10.1016/s0014-4827(66)80149-0. [DOI] [PubMed] [Google Scholar]
  4. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  5. Jeon K. W. Simple method for staining and preserving epoxy resin-embedded animal tissue sections for light microscopy. Life Sci. 1965 Oct;4(19):1839–1841. doi: 10.1016/0024-3205(65)90064-0. [DOI] [PubMed] [Google Scholar]
  6. Lenghaus C., Studdert M. J. Relationships of canine panleucopaenia (enteritis) and myocarditis paroviruses to feline panleucopaenia virus. Aust Vet J. 1980 Mar;56(3):152–153. doi: 10.1111/j.1751-0813.1980.tb05663.x. [DOI] [PubMed] [Google Scholar]
  7. O'Reilly K. J., Whitaker A. M. The development of feline cell lines for the growth of feline infectious enteritis (panleucopaenia) virus. J Hyg (Lond) 1969 Mar;67(1):115–124. doi: 10.1017/s0022172400041498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. O'Shea J. D., Studdert M. J. Growth of an autonomously replicating parvovirus (feline panleukopenia): kinetics and morphogenesis. Arch Virol. 1978;57(2):107–122. doi: 10.1007/BF01315672. [DOI] [PubMed] [Google Scholar]
  9. PUCK T. T., STEFFEN J. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION. Biophys J. 1963 Sep;3:379–397. doi: 10.1016/s0006-3495(63)86828-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rhode S. L., 3rd, Paradiso P. R. Parvovirus genome: nucleotide sequence of H-1 and mapping of its genes by hybrid-arrested translation. J Virol. 1983 Jan;45(1):173–184. doi: 10.1128/jvi.45.1.173-184.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rhode S. L., 3rd Replication process of the parvovirus H-1. I. Kinetics in a parasynchronous cell system. J Virol. 1973 Jun;11(6):856–861. doi: 10.1128/jvi.11.6.856-861.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Studdert M. J., Peterson J. E. Some properties of feline panleukopenia virus. Arch Gesamte Virusforsch. 1973;42(4):346–354. doi: 10.1007/BF01250715. [DOI] [PubMed] [Google Scholar]
  14. Studdert M. J. Restriction endonuclease DNA fingerprinting of respiratory, foetal and perinatal foal isolates of equine herpesvirus type 1. Arch Virol. 1983;77(2-4):249–258. doi: 10.1007/BF01309272. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES