Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1985 Feb;53(2):384–390. doi: 10.1128/jvi.53.2.384-390.1985

Identification of an enveloped phage, mycoplasma virus L172, that contains a 14-kilobase single-stranded DNA genome.

K Dybvig, J A Nowak, T L Sladek, J Maniloff
PMCID: PMC254648  PMID: 2982026

Abstract

We have found that mycoplasma virus L172 is an enveloped globular virion containing circular, single-stranded DNA of 14.0 kilobases. L172 has been reported by other workers to have a double-stranded DNA genome of 13 to 17 kilobase pairs and has been classified as a plasmavirus, a group for which mycoplasma virus L2 is the type member. Mycoplasma viruses L172 and L2 differ in genome size and structure, DNA base composition, and protein composition, and they have no detectable DNA homology. As the only reported enveloped virion containing single-stranded DNA, L172 represents a new group of viruses.

Full text

PDF
384

Images in this article

385Image
on p.385
386Image
on p.386
387Image
on p.387
387Image
on p.387
387Image
on p.387

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  2. Drásil V., Doskar J., Viklická S., Tkadlecek L. Size and shape of mycoplasma virus MV-Lg-L 172 DNA. Acta Virol. 1978 Nov;22(6):443–450. [PubMed] [Google Scholar]
  3. Dybvig K., Maniloff J. Integration and lysogeny by an enveloped mycoplasma virus. J Gen Virol. 1983 Aug;64(Pt 8):1781–1785. doi: 10.1099/0022-1317-64-8-1781. [DOI] [PubMed] [Google Scholar]
  4. Dybvig K., Swinton D., Maniloff J., Hattman S. Cytosine methylation of the sequence GATC in a mycoplasma. J Bacteriol. 1982 Sep;151(3):1420–1424. doi: 10.1128/jb.151.3.1420-1424.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gourlay R. N. Mycoplasmatales virus-laidlawii 2, a new virus isolated from Acholeplasma laidlawii. J Gen Virol. 1971 Jul;12(1):65–67. doi: 10.1099/0022-1317-12-1-65. [DOI] [PubMed] [Google Scholar]
  6. Gourlay R. N., Wyld S. G., Garwes D. J., Pocock D. H. Comparison of mycoplasmatales virus MV-Lg-pS2-L172 with plasmavirus MV-L2 and the other mycoplasma viruses. Arch Virol. 1979;61(4):289–296. doi: 10.1007/BF01315015. [DOI] [PubMed] [Google Scholar]
  7. Haberer K., Klotz G., Maniloff J., Kleinschmidt A. K. Structural and biological properties of mycoplasmavirus MVL3: an unusual virus-procaryote interaction. J Virol. 1979 Oct;32(1):268–275. doi: 10.1128/jvi.32.1.268-275.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Liska B., Tkadlecek L. Electron-microscopic study of a Mycoplasmatales virus, strain MV-Lg-pS2-L 172. Folia Microbiol (Praha) 1975;20(1):1–7. doi: 10.1007/BF02877079. [DOI] [PubMed] [Google Scholar]
  9. Liss A., Maniloff J. Infection of Acholeplasma laidlawii by MVL51 virus. Virology. 1973 Sep;55(1):118–126. doi: 10.1016/s0042-6822(73)81013-x. [DOI] [PubMed] [Google Scholar]
  10. Liss A., Maniloff J. Isolation of Mycoplasmatales viruses and characterization of MVL1, MVL52, and MVG51. Science. 1971 Aug 20;173(3998):725–727. doi: 10.1126/science.173.3998.725. [DOI] [PubMed] [Google Scholar]
  11. Maniloff J., Cadden S. P., Putzrath R. M. Maturation of an enveloped budding phage: mycoplasmavirus L2. Prog Clin Biol Res. 1981;64:503–513. [PubMed] [Google Scholar]
  12. Maniloff J., Haberer K., Gourlay R. N., Das J., Cole R. Mycoplasma viruses. Intervirology. 1982;18(4):177–188. doi: 10.1159/000149323. [DOI] [PubMed] [Google Scholar]
  13. Nowak J. A., Das J., Maniloff J. Characterization of an Acholeplasma laidlawii variant with a REP- phenotype. J Bacteriol. 1976 Aug;127(2):832–836. doi: 10.1128/jb.127.2.832-836.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nowak J. A., Maniloff J. Physical characterization of the superhelical DNA genome of an enveloped mycoplasmavirus. J Virol. 1979 Jan;29(1):374–380. doi: 10.1128/jvi.29.1.374-380.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Putzrath R. M., Cadden S. P., Maniloff J. Effect of cell membrane composition on the growth and composition of a nonlytic enveloped mycoplasmavirus. Virology. 1980 Oct 15;106(1):162–167. doi: 10.1016/0042-6822(80)90235-4. [DOI] [PubMed] [Google Scholar]
  16. Putzrath R. M., Maniloff J. Properties of a persistent viral infection: possible lysogeny by an enveloped nonlytic mycoplasmavirus. J Virol. 1978 Oct;28(1):254–261. doi: 10.1128/jvi.28.1.254-261.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sladek T. L., Maniloff J. Polyethylene glycol-dependent transfection of Acholeplasma laidlawii with mycoplasma virus L2 DNA. J Bacteriol. 1983 Aug;155(2):734–741. doi: 10.1128/jb.155.2.734-741.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Zasloff M., Ginder G. D., Felsenfeld G. A new method for the purification and identification of covalently closed circular DNA molcules. Nucleic Acids Res. 1978 Apr;5(4):1139–1152. doi: 10.1093/nar/5.4.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES