Cell Cycle-Dependent Ciliogenesis and Cancer

Abstract

In mammals, most cell types have primary cilia, protruding structures involved in sensing mechanical and chemical signals from the extracellular environment, that act as major communication hubs for signaling controlling cell differentiation and polarity. The list of clinical disorders associated with ciliary dysfunction has expanded from polycystic kidney disease (PKD) to include many others. Transformed cells commonly lack cilia, but whether this lack is cause or consequence of transformation is not well understood. We here discuss work addressing recently identified actions of the cancer-promoting proteins Aurora A and HEF1/NEDD9/CAS-L at cilia. Together with older studies, this work suggests loss of cilia in cancer may contribute to the insensitivity of cancer cells to environmental repressive signals, based in part on derangement of cell cycle checkpoints governed by cilia and centrosomes.

Introduction

Immotile primary cilia are found on most epithelial and stromal cells in the mammalian body. The primary cilium is a centriole-based organelle that consists of (9+0) microtubule pairs located at the plasma membrane. The past decade has seen exponential growth in studies of the cilium, based on the recognition that numerous developmental defects and chronic diseases arise from aberrant formation or function of cilium (1). The idea of the cilium as 'cellular cybernetic probe' ((2), i.e. force and small molecule-sensing protrusion) capable of transducing environmental information from the extracellular matrix to the centrosome, and suggesting a matrix-cilium-Golgi signaling continuum (2) was first proposed in the 1990s. This model is now supported by extensive data, and informs studies of “ciliary diseases” such as PKD, Bardet-Biedl syndrome, and Kartagener’s syndrome.

A correlation between cellular transformation and loss of a primary cilium has been noted for over a decade (3). It has been proposed that cancer cells lacking cilia reduce or alter their response to extracellular cues that regulate growth and differentiation. A number of recent studies have shown that a number of critical cell signaling and adhesion molecules are clustered on the cilium, and require an intact cilium for normal function (Figure 1A). These include components of the Shh (Sonic Hedgehog), PCP (planar cell polarity), PDGFRα (platelet derived growth factor receptor α) and VHL/GSK3β (von Hippel-Lindau, and glycogen synthase kinase 3 β) pathways (4–6). For at least VHL/GSK3β, deregulated expression of the signaling protein has been found to influence the ability of cells to form cilia (7). Nevertheless, the significance of ciliary loss for influencing signaling in cancer development is largely unclear.

Figure 1.

A. The cilium as an environmental rheostat: coordination of cancer-related signaling molecules. PDGF-AA binding to the PDGFRαα receptor located at the ciliary membrane induces phosphorylation and activation of the Akt and Mek1/2-Erk1/2 signaling pathways. Sonic hedgehog (Shh) binding to Patched (Ptch) relieves inhibition of the Smoothened protein (Smo), and initiates a signaling cascade that result in activation of the Gli family of zinc-finger transcription factors. Localization of the Smo receptor to primary cilia is important for this signaling cascade. Aberrant Shh signaling was recently observed in a variety of epithelial cancers, and it has been shown that the Shh target Gli1 regulates expression of Snail, a gene important for epithelial-mesenchymal transition (EMT). pVHL and GSK-3β also localize to primary cilia, and act together to maintain the primary cilium: their combined inactivation leads to loss of cilia, and correlates with renal tumor progression.

B. Centrosome serves as the signaling molecules hub licensing cell cycle progression. As cells emerge from quiescence and resorb cilia, the CDK2-cyclin E complex progressively associates with the centrosome and triggers the G1/S transition. Centrosome duplication and DNA synthesis are tightly connected through cyclin E/Cdk2 activation. Overexpression of IFT88 prevents G1-S transition, while depletion of IFT88 induces cell cycle progression. The cell cycle checkpoint regulator p53 evaluates centrosome integrity in cell cycle. p53 accumulation at the centrosome is preceded by p38-dependent phosphorylation, and is important for execution of G1/S arrest. Deletion or mutations in p53 gene were found in over 50% of all human cancers: consequences if any for ciliary integrity are not known.

C. Model for HEF1– Aurora A – HDAC6 signaling at cilia. Extracellular factors bind to growth factor receptors (GFR) to induce HEF1. HEF1 binds to Aurora A kinase, promoting its activation. Aurora A in turn phosphorylates and activates cilia-associated HDAC6. Moving along the ciliary axomene, HDAC6 deacetylates substrates, causing ciliary resorption. These events occur as an early transient response of G0/G1 cells to serum treatment, as well as at G2/M transition in response to intrinsic signaling cues.

Overexpression of HEF1 is associated with metastatic progression of melanoma and lung cancer (8, 9), while Aurora A overexpression is a common oncogenic lesion in multiple cancer types (10). Overexpression of HEF1 and Aurora A promotes cytokinetic failure, a contributor to genomic instability; overexpression of HEF1 also induces tumor cell migration and invasion. As discussed below, the recent definition of a HEF1 – Aurora A – HDAC6 signaling axis governing the resorption of cilia in addition to the previously defined roles for these proteins suggests a novel molecular mechanism to explain at least some cancer-associated ciliary loss. We propose that enhanced intracellular levels of HEF1 and Aurora A may also interfere with a normal cellular interconversion between cilia and centrosome that helps time cell cycle by staging critical signaling complexes that govern emergence from quiescence, entry to S phase, and initiation of M phase. Through these actions, HEF1 and Aurora A can provide multiple stimuli to cancer aggressiveness.

Cell-cycle dependent ciliogenesis

The transition between centrosome and cilium, and the regulation of cell cycle control, are closely linked in normal dividing cells. Centrosomes, which are composed of two barrel-shaped centrioles surrounded by pericentriolar material, organize the cytoplasmic microtubule network during interphase, and the bipolar spindle during mitosis. In post-mitotic cells, the centrosome migrates to the cell surface, and one of the centrioles differentiates into a basal body that nucleates microtubules to form a cilium (11). In normal proliferating cells, the presence of a cilium on the cell surface is transient: most commonly observed in G1, and usually resorbed before or during G2 phase. In asynchronous populations, typically 10–25% of cells have observable cilia (12). As cultured cells become quiescent, an increasing proportion of the population is ciliated, attaining >90% in some cell types. This quiescent ciliated state parallels the natural state of normal cells organized in tissues in vivo, and persists until environmental changes trigger cell cycle re-entry.

An increasing number of factors controlling the formation or loss of cilia in normal cell cycle are being found to influence cell cycle. Regarding ciliary formation, removal of a number of intraflagellar transport (IFT) proteins causes severe ciliary assembly defects or complete loss of cilium (13). Some of the IFT proteins, long thought to solely be relevant to protein trafficking within cilia, have recently been found to function in cell cycle control. For example, reduced levels of IFT27 results in cell growth inhibition, and incomplete or asymmetric cytokinesis (14). Overexpression of IFT88 prevents G1-S transition, while depletion of IFT88 causes cilium disappearance and promotes cell cycle progression (15). As the ciliary membrane is strongly enriched with growth factor, chemo- and mechano-sensing receptors (13, 16), absence of cilia may partly influence cell cycle progression by limiting the ability of cells to respond to external cues.

As an alternative explanation, some evidence suggests that misregulation of the basal body-centrosome transition may trigger cell cycle checkpoints. A centrosome is becoming well-established as an organization center for signaling proteins that drive S-phase progression (17), emphasized by studies demonstrating loss of cell cycle progression following centrosome ablation (18). An early study suggested that at least in some model systems, centrioles encompassed within a ciliary basal body do not appear to be competent to organize the factors necessary to progress through S phase (19). Rather, the basal body “differentiates” back into a centrosome prior to performing these functions, which include spatially coordinating the activation of the Cdk2/cyclin E kinase complex (20), necessary both for centrosome duplication and DNA synthesis (21). However, as mice lacking Cdk2 are phenotypically normal, with only a delay in S-phase entry by Cdk2−/− cells (22), and as some cell types can progress through S phase although their centrosomes are entirely ablated (18), activation of centrosomal Cdk2/cyclin E is not essential in all cell types, potentially due to redundant pathways. It has also been proposed that overexpressed IFT88 induces cell cycle arrest at least in part by activating the Rb growth suppressor (15). Interestingly, a recent detailed analysis has demonstrated that siRNA depletion of a panel of proteins required for core centrosomal structural integrity both induces G1 arrest by triggering a p53/p38-dependent checkpoint, and inhibits formation of primary cilia (23) Figure 1B. The relative priority of these events in the arrest process requires further study.

Although some cell types transition through S phase without resorbing cilia, almost no examples exist where ciliated cells enter mitosis, suggesting a second restriction point of intact cilia on cell cycle (12). In a number of systems, a centrosome is not necessary for spindle formation (24) or mitotic entry, although an important centrosomal role in timing mitotic entry based on spatially coordination activation of the Aurora A kinase to allow nuclear envelope breakdown has recently been described (25). It is possible that a mother centriole sequestered in a basal body has an actively inhibitory role on mitotic entry, although no experiments to date have directly tested this idea. Suggestively, a cilia-associated member of the NIMA family of cell cycle kinases, Fa2p, is necessary for both cell division-regulated ciliary resorption, and cell cycle progression from G2 to M phase (16, 26).

Clearly, while a growing body of evidence links cilia function and cell cycle in normal cells, none of the preceding studies have closely implicated the ciliary resorption/protrusion cycle with changes relevant to cancer. In the context of the above discussion, the typical absence of cilia from transformed cells suggest several possible interpretations for a cancer-promoting role of the altered state: (1) it may be beneficial to some classes of tumor cells to limit responsiveness to external cues that promote differentiation over cycling; and (2) the continual presence of centrioles in a centrosomal rather than ciliary context may remove cilia-initiated cell cycle restrictions. Such restriction cannot be universal for mammalian cancer; some cell types, notably hematopoietic cells, are non-ciliated. However, particularly for cancers associated with aberrant signaling involving PDGF, Wnt, or Hedgehog signaling, such a ciliary contribution may be key. To better assess such a model, it is important to understand the factors controlling ciliary loss in cancer.

HEF1-dependent activation of oncogenic Aurora A kinase induces ciliary disassembly

Aurora A is a centrosomally localized cell cycle regulatory serine/threonine kinase that activates the cyclin B1-Cdk1 mitotic kinase, and coordinates formation of a bipolar spindle and nuclear envelope breakdown in M phase (25). Aurora A is overexpressed due to gene amplification in many types of human cancer (10). In both naturally occurring tumors, and with ectopic overexpression systems, increased Aurora A correlates with induction of aneuploidy, centrosomal anomalies, tumor invasiveness, and poor prognosis. It has recently been proposed that increased Aurora A expression is the major contributing factor to the elevated instability and aneuploidy of p53 null tumors (27).

HEF1 has recently emerged as a pro-metastatic factor. Elevated expression of HEF1 marks melanomas, a subset of lung cancers, and other cancers, and for melanomas and glioblastomas, HEF1 clearly drives invasive behavior (9, 28). A significant amount of work over the past 12 years has elucidated complex HEF1 roles at focal adhesions in regulation of migration, apoptosis, and chemokine response (29, 30), processes relevant to metastasis. However, in 2005, a centrosomal pool of HEF1 was found to bind and help activate the Aurora A kinase at mitotic entry (31), linking function of the two proteins. Increased HEF1 expression produced similar defects in cytokinesis and centrosome amplification as increased levels of Aurora A, suggesting a potential role for HEF1 in promoting aneuploidy in metastatic tumors.

Providing a critical link to the idea of a role for HEF1 and Aurora A in the ciliary cycle, in 2004 Snell and co-workers had determined that CALK, a Chlamydomonas protein that is distantly related to Aurora A, governs resorption of the flagellum, an organelle similar to the mammalian cilium (32). Intriguingly, in Chlamydomonas CALK is not solely a mitotic kinase, but functions throughout cell cycle in response to a variety of cues inducing loss of flagella. Pursuing these hints, we subsequently demonstrated HEF1-dependent activation of Aurora A upon induction of ciliary resorption by growth factor treatment of quiescent cells ((33), Figure 1C). We also established the requirement for Aurora A kinase for cilium disassembly not only during mitosis, but also in G1 cells emerging from quiescence: the first time a non-mitotic activity for Aurora A had been identified in mammalian cells, and paralleling activity in Chlamydomonas. Further, microinjection of Aurora A into cells resulted in almost instantaneous cilium disappearance, leading to the conclusion that active Aurora A is necessary and sufficient to induce cilium resorption. Interestingly, a recent study of male infertility has established a mutation leading to loss of expression of the Aurora A-related kinase, Aurora C caused production of large-headed, multiflagellar spermatozoa (34), indirectly suggesting a related activity.

Moving downstream, Aurora A specifically phosphorylates the tubulin deacetylase HDAC6, dramatically increasing in HDAC6 deacetylase activity. Inhibition of HDAC6 activity by the small molecule inhibitor tubacin, or gene silencing of HDAC6 by siRNA, in each case resulted in cilium stabilization, thus establishing HDAC6 as one important downstream targets of Aurora A kinase involved in cilium destabilization. HDAC6 activity is most simply explained through action regulating the ciliary axoneme, removing stabilizing acetylation marks on axonemal α-tubulin. However, some earlier studies in Chlamydomonas have argued that changes in the acetylation status of α-tubulin do not influence flagellar resorption (35) hence, either Chlamydomonas and mammals may be different in this regard, or HDAC6 may target additional or alternative substrates in this process. At least indirectly, one such target may be Aurora A itself; a very recent study has shown inhibition of HDAC6 caused destabilization of Aurora A kinase via disruption of an HSP90-Aurora A complex (36). It is likely that additional Aurora A and HDAC6 targets will be identified, with IFT proteins likely candidates for activity-altering modification.

Tipping the cilium-centrosome balance as a pro-cancer stimulus?

As reviewed above, a growing body of data suggests that structural and signaling integrity of the cilium are important for regulation of a normal cell cycle. These data indicate timed resorption and reassembly of cilia are dynamic processes closely linked to execution of cell developmental programs, and suggest the following speculative model (Figure 1C): in quiescent, G0/G1 cells of lineages that are typically ciliated, receptors for growth factors (GF) or other differentiation control signals (eg.PDGFR/Ptc-Smo) are displayed on the ciliary membrane. Upon stimulation of these receptors, a signal is transported down to the command center at the basal body. This induces “firing” of the Aurora A kinase, promoting local activation of HDAC6, transporting it along axoneme to destabilize microtubules and/or influence the activity of IFTs. Again speculatively, in normal cells organized in tissues (not actively proliferating), one purpose of transient Aurora A-HDAC6 activation following receipt of transient growth factor stimulation may be to control ciliary length. Fine control of ciliary length may serve as a rheostat, increasing or decreasing the ability of cells to respond to growth factors by limiting area for display of cilia-associated receptors. In contrast, strong activation of Aurora A and HDAC6 is sufficient to induce full ciliary resorption and remove basal body-dependent restrictions on cell cycle progression, with the basal body centriole differentiating to a centrosome competent to fully coordinate S- and M-phase entry. Finally, abnormal overexpression and/or activation of key components of this resorption signaling machinery such as HEF1 and Aurora A enforces ciliary disassembly under all conditions, distorting the responsiveness of cells to a subset of external growth regulatory cues (37), and inducing excessive cell proliferation and migration.

The relations between control of ciliary integrity, ciliary signaling, and cancer clearly require further study. Provocatively, besides the role of cilia in coordinating PDGFα and Hedgehog signaling, disruption of the ciliary basal body has very recently been shown to also cause defective signaling in the Wnt pathway: Wnt signaling is commonly perturbed in many cancers. In lung cancer metastases dependent on loss of the tumor suppressor LKB1, HEF1 is strongly upregulated (8): interestingly, LKB1 also regulates Wnt signaling (38), and mutational inactivation of LKB1 in development phenocopies loss of VHL, another regulator of cilia (39). A common role of LKB1, Wnt, and VHL is in the regulation of planar cell polarity (PCP). PCP control is extremely important in known ciliary diseases such as PKD, with aberrant mitotic orientation found to contribute to renal tubular enlargement and cyst formation (40). It seems likely that LKB1 might be an upstream regulator of ciliary resorption through control of HEF1, Aurora A, and HDAC6. Whether HEF1, Aurora A, and HDAC6 in turn influence PCP via actions at centrosomes and cilia is an as-yet uninvestigated but intriguing possibility.

Based on the well-established role of Aurora A and HDAC6 in regulating mitosis, several inhibitors targeting these proteins have been developed and have entered Phase I clinical trials for advanced cancer treatment. The new ciliary functions of Aurora A kinase and HDAC6 suggest the necessity of a more complicated interpretation of the output of Aurora A- and HDAC-directed therapeutics. Further research directed at understanding the relationship between the cilium, cell-cycle, and cilia-mediated signaling is likely to reveal new insights into the pathogenesis of many types of cancer, and nominate additional cilia-associated proteins as new targets for cancer therapeutics.