β1,2-Xylosyltransferase Cxt1p Is Solely Responsible for Xylose Incorporation into Cryptococcus neoformans Glycosphingolipids

Abstract

The Manα1,3(Xylβ1,2)Manα structural motif is common to both capsular polysaccharides of Cryptococcus neoformans and to cryptococcal glycosphingolipids. Comparative analysis of glycosphingolipid structural profiles in wild-type and mutant strains showed that the Xylβ1,2-transferase (Cxt1p) that participates in capsular polysaccharide biosynthesis is also the sole transferase responsible for adding xylose to C. neoformans glycosphingolipids.

The pathogenic basidiomycete Cryptococcus neoformans causes serious disease in immunocompromised patients. A dominant feature of C. neoformans is its polysaccharide capsule, which is required for virulence. Xylose is a key component of both of the major polysaccharides comprising the capsule, glucuronoxylomannan (GXM) and galactoxylomannan (GalXM), and is essential for proper capsule formation and virulence (7, 16). Xylose is also a feature of C. neoformans glycosylinositol phosphorylceramides (GIPCs) (9), glycosphingolipids characteristic of fungi (4, 12). Fungal GIPCs differ fundamentally from mammalian glycosphingolipids in terms of structure, and their biosynthesis is essential for normal growth and life cycle (3, 5), suggesting they could be exploited for diagnostic (18) and therapeutic strategies (6, 17, 19). Interestingly, specific structural features are shared between the GIPCs and the capsular polysaccharides of C. neoformans. The GIPC core structure has the overall sequence Manα3(Xylβ2)Manα4Galβ6Manα2InsPCer (9). Like the structures of both GXM and GalXM, this structure includes a branching Xylβ1,2 residue linked to the reducing mannose of the Manα1,3Manα motif. In a more extensive parallel to GalXM, the xylosylated α-mannose of the GIPC core is 1,4-linked to β-galactose.

We have recently identified a cryptococcal Xylβ1,2-transferase (Cxt1p) that acts in synthesizing both capsule polysaccharides (11). Cells in which CXT1 has been deleted (cxt1Δ::ΝΑΤ [10]) show a 30% reduction in β1,2-Xyl addition to GXM and more than 90% reduction in β1,2-xylose addition to GalXM (10). In light of the structural homologies mentioned above, we tested the hypothesis that this enzyme also adds xylose to GIPCs. To do this, we compared GIPCs from a wild-type strain (JEC21) with those of the cxt1Δ mutant cells. As a control, we used a strain which bears the deletion of UXS1, the gene encoding UDP-GlcA decarboxylase (1, 16). This strain (uxs1Δ::ADE2) cannot synthesize the xylose donor UDP-xylose and is therefore globally deficient in the xylose modification of all glycoconjugates.

The C. neoformans JEC21, uxs1Δ (generously provided to the Doering laboratory by Guilhem Janbon) (16), and cxt1Δ strains (10) were grown at 30°C with rotation (200 rpm). All of these strains are closely related serotype D MATα strains: the cxt1Δ strain was generated directly from JEC21, and the uxs1Δ strain was made from JEC155, which is derived from a related serotype D MATa strain (JEC20). For lipid preparations, cells were cultured in YPD medium (1% yeast extract, 2% Bacto peptone, 2% glucose) for 3 days, collected by centrifugation (6,000 rpm; 10 min; 4°C), washed once with cold water and twice with cold 20 mM sodium azide, and frozen. The frozen cell pellet (50 to 70 g [wet weight]) was then homogenized with 6 volumes of chloroform-methanol, 1:1 (vol/vol), and solvents were evaporated. Enrichment of glycosphingolipids, recovery of acidic fractions containing GIPCs by ion-exchange chromatography, and purification of GIPCs by high-performance liquid chromatography were then performed as described previously (2, 18). Crude acidic fractions were analyzed by high-performance thin-layer chromatography (HPTLC) on silica gel no. 60 plates (E. Merck, Darmstadt, Germany) developed in chloroform-methanol-water (60:40:9 [vol/vol/vol], containing 0.002% [wt/vol] CaCl₂), with hexose-containing components detected by Bial's orcinol reagent.

Figure 1 shows the HPTLC profiles of GIPCs from the wild-type, the uxs1Δ, and the cxt1Δ cells. Each of the three strains exhibits a pair of bands that comigrates with a fungal Manα2InsPCer (MIPC) standard; the differences in the relative distribution of the bands between monohydroxy (upper) N-acyl forms and the dihydroxy (lower) N-acyl forms may reflect strain variation. (Consistent with this idea, the distribution of MIPC bands in the cxt1Δ strain is similar to that of the bands in JEC21, its immediate parent strain [Fig. 1, lanes 3 and 1, respectively]; distribution in the uxs1Δ strain [Fig. 1, lane 2] is somewhat altered.) Significantly, the wild-type cells also express major low-mobility components (Cn-5 and Cn-6) in a darkly stained band that is completely absent from both the cxt1Δ and the uxs1Δ cells. In contrast, the dominant product in the cxt1Δ strain is a pair of higher-mobility components (Cn-4) whose migration is consistent with that of compounds that are less polar than those of the major wild-type species. The pattern in the uxs1Δ strain, the control strain completely lacking UDP-xylose, is nearly identical. While this work was in progress, Gutierrez et al. (8) reported that the major GIPC species in the uxs1Δ cells is a truncated compound, Manα3Manα4Galβ6Manα2InsPCer. The production of the same species in the cxt1Δ cells (Fig. 1) demonstrates that Cxt1p is responsible for the transfer of all xylose to cryptococcal GIPCs.

FIG. 1.

HPTLC profiles of GIPCs (orcinol-stained acidic lipid fractions) from the C. neoformans strains indicated at the top of the lanes, along with authentic Manα2InsPCer (MIPC) standard from Aspergillus fumigatus (lane S). WT, wild-type strain JEC21. The origin is indicated by the line at the bottom of the image.

We next performed structural studies to confirm the HPTLC comigration and identities of dominant products from the uxs1Δ and cxt1Δ strains. We first performed electrospray ionization mass spectrometry (ESI-MS and ESI-MSⁿ) to analyze crude and purified GIPC fractions in the positive ion mode (⁺ESI), using a linear ion trap instrument (LTQ; Thermo Finnigan, San Jose, CA). Results were interpreted essentially as described in previous reports of purified GIPC components (2, 13-15, 18). Molecular profiles of C. neoformans GIPCs as [M(Na)+Na]⁺ salt adducts were first acquired via ⁺ESI-MS. The compositions of the major molecular species in the JEC21 profile (not shown) were consistent with those of HexInsPCer (m/z 1,132, 1,148), Hex₄PenInsPCer (m/z 1,750, 1,766), and Hex₅PenInsPCer (m/z 1,912, 1,928). These results correspond to those of the previously characterized Manα2InsPCer (Cn-1), Manα3(Xylβ2)Manα4Galβ6Manα2InsPCer (Cn-5), and Manα6Manα3(Xylβ2)Manα4Galβ6Manα2InsPCer (Cn-6) GIPC sequences (9). The m/z difference of 16 between molecular adduct pair members is consistent with the differences between the degrees of hydroxylation of the ceramide fatty-N-acyl group (t18:0 4-hydroxy-sphinganine with h24:0 and h₂24:0 fatty acids, respectively). These results show that the low R_f band in the wild-type HPTLC profile (Fig. 1) corresponds to four components, Cn-5 and Cn-6, in an approximately 1:1 ratio, each bearing two types of ceramide. The profile of JEC21, thus, differs somewhat from those of the wild-type strains previously characterized (strain 444, which expressed Cn-5 almost exclusively, and strain KN99, which expressed Cn-6 almost exclusively [8, 9]).

The uxs1Δ and cxt1Δ mutant profiles are shown in Fig. 2A and B. Both profiles exhibit pairs of [M(Na)+Na]⁺ salt adduct ions consistent with the HexInsPCer (m/z 1,132, 1,148) and Hex₄InsPCer (m/z 1,618, 1,634) compositions, corresponding to previously characterized GIPC sequences Manα2InsPCer (Cn-1) and Manα3Manα4Galβ6Manα2InsPCer (Cn-4) (8). We detected traces of components with intermediate numbers of Hex residues in the mutant GIPC profiles, but no trace of xylosylated GIPC products were detected in either mutant profile. These results are summarized in Table 1.

FIG. 2.

Mass spectrometry of GIPC fractions. (A and B) ⁺ESI-MS¹ profiles (as [M(Na)+Na]⁺ salt adducts) of crude GIPC fractions from the uxs1Δ and cxt1Δ strains. (C) ⁺ESI-MS² (m/z 1,586 →) spectrum of selected [M(Li)+Li]⁺ salt adduct m/z 1,586 (Hex₄InsPCer, corresponding to the [M(Na)+Na]⁺ salt adduct m/z 1,618 to 1632) in panel B. (D) ⁺ESI-MS³ (m/z 1,586 → 921 →) spectrum originating from the same molecular species.

TABLE 1.

ESI-MS and ESI-MS² data for cryptococcal GIPCs^a

Strain and GIPC	GIP t18:0/(fa)	MW (u) M(H)	m/z
			[M(Na)+Na]+	[M(Li)+Li]+	[Cer+Li]+	[GIP(Li)+Li]+
WT (JEC21)
Cn-1	HexInsP				435
	(h24:0)	1,087	1,132	1,100		690
	(h224:0)	1,103	1,148	1,116		706
Cn-5	Hex4PenInsP				1,053
	(h24:0)	1,705	1,750	1,718		690
	(h224:0)	1,721	1,766	1,734		706
Cn-6	Hex5PenInsP				1,215
	(h24:0)	1,867	1,912	1,880		690
	(h224:0)	1,883	1,928	1,896		706
uxs1Δ and cxt1Δ mutants
Cn-1	HexInsP				435
	(h24:0)	1,119	1,132	1,100		690
	(h224:0)	1,135	1,148	1,116		706
Cn-4	Hex4InsP				921
	(h24:0)	1,573	1,618	1,586		690
	(h224:0)	1,589	1,634	1,602		706

^aMW, molecular weight; M(H), protonated uncharged molecular species; WT, wild type.

To confirm the lack of xylose in mutant GIPC molecular species, each molecular adduct in both profiles was selected for further fragmentation by the ⁺ESI-MSⁿ mode. To improve fragmentation, we treated the samples with lithium iodide, which converts GIPC molecular species to lithium salt adducts, [M(Li)+Li]⁺ (2, 13, 15, 18); this also reduces the m/z of each molecular species by 32 compared with [M(Na)+Na]⁺ (Table 1). A ⁺ESI-MS² spectrum acquired from the [M(Li)+Li]⁺ peak at m/z 1,586 (corresponding to the [M(Na)+Na]⁺ m/z 1,618) of the cxt1Δ mutant profile (Fig. 2C) showed the predominant glycosylinositol phosphate (GIP) fragment pair [B₅PO₃(Li)+Li]⁺/[C₅PO₃(Li)+Li]⁺ (m/z 921/903, respectively) corresponding to Hex₄InsP and other fragments from glycosidic cleavages (Fig. 3; Table 2). A ceramide ion ([Y₀+Li]⁺) was observable at m/z 690 (the h24:0/t18:0 lipoforms, not marked). An ⁺ESI-MS³ spectrum acquired from the [C₅PO₃(Li)+Li]⁺ ion at m/z 921 (m/z 1,586 → 921 →) (Fig. 2D) showed that all of the glycosidic cleavages were consistent with those of a linear Hex₄InsP primary fragment (Fig. 3; Table 2). Essentially identical spectra were acquired from the [M(Li)+Li]⁺ salt adduct at m/z 1,602 (corresponding to the [M(Na)+Na]⁺ m/z 1,634; not shown), except that the ceramide ion was observed in the MS² spectrum at m/z 706 (the h₂24:0/t18:0 lipoforms). Essentially identical results were obtained from the corresponding pair of [M(Li)+Li]⁺ salt adducts in the uxs1Δ strain profile (not shown).

FIG. 3.

Fragmentation of Manα3Manα4Galβ6Manα2InsPCer (Cn-4) in the modes ⁺ESI-MS² and ⁺ESI-MS³.

TABLE 2.

Product ions formed in low-energy ESI-MS² and ESI-MS³ spectra^a

m/z		Cn-4 assignment
h24:0	h224:0
1,586	1,602	[M(Li)+Li]+
1,568	1,586	[M(Li)+Li−H2O]+
1,424	1,440	[M(Li)−Hex+Li]+
1,262	1,278	[M(Li)−2Hex+Li]+
1,230	1,246	[J(Li)+Li−H2O]+
1,100	1,116	[M(Li)−3Hex+Li]+
921		[C5PO3(Li)+Li]+
903		[B5PO3(Li)+Li]+
835		[C5+Li]+
817		[B5+Li]+
759		[Y4/C5PO3(Li)+Li]+
741		[Y4/B5PO3(Li)+Li]+
690	706	[Y0+Li]+
673		[Y4/C5+Li]+ or [C4+Li]+
672		[Z0+Li]+
655		[Y4/B5+Li]+ or [B4+Li]+
597		[Y3/C5PO3(Li)+Li]+
579		[Y3/B5PO3(Li)+Li]+
511		[Y3/C5+Li]+ or [Y4/C4+Li]+ or [C3+Li]+
493		[Y3/B5+Li]+ or [Z3/C5+Li]+ or [Y4/B4+Li]+ or [Z4/C4+Li]+ or [B3+Li]+
435		[Y2/C5PO3(Li)+Li]+
417		[Y2/B5PO3(Li)+Li]+
349		[C2+Li]+ or any equivalent two-residue segment
331		[C2+Li]+ or any equivalent two-residue segment

^aData show m/z of product ions formed in low-energy ESI-MS² and ESI-MS³ spectra of lithium adducts of tetraglycosylinositol phosphorylceramide from C. neoformans GIPC (Cn-4). Base peaks are in boldface type, and fragment designations are shown as in references 2, 13, 15 and 18 and in Fig. 3.

Our data show that the lack of a single xylosyltransferase, Cxt1p, results in the complete absence of xylose from GIPCs of cryptococcal cells. This loss of xylose yields glycolipids that are indistinguishable from those formed in the uxs1Δ cells, where no xylose can be added to any glycans. The absence of residual xylose-containing GIPCs in the cxt1Δ mutant further indicates that no other enzyme performs the function of xylose addition during GIPC synthesis. In agreement with findings described by Gutierrez et al. (8), we observed that the lack of xylose modification of GIPC structures is accompanied by truncation of the terminal mannose residues distal to the branch.

Using in vitro assays, we have found that Cxt1p transfers xylose in β1,2 linkage to a Manα3Man disaccharide (11); our recent in vivo results further show that the cxt1Δ mutant is partially deficient in the transfer of Xylβ2 to both GXM and GalXM (10). Together, these data and the current studies demonstrate that Cxt1p is an unusual multiple-function xylosyltransferase that acts in three fundamental processes of C. neoformans: GXM synthesis, GalXM synthesis, and GIPC synthesis. The critical importance of the addition of xylose to cryptococcal biology and virulence (7, 16) suggests that this fungus-specific protein warrants further investigation, in particular with respect to its role in cryptococcal pathogenesis.