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. Author manuscript; available in PMC: 2008 Sep 22.
Published in final edited form as: Biochemistry. 2006 Apr 4;45(13):4231–4239. doi: 10.1021/bi052064y

Figure 4.

Figure 4

Treatment of non-expressing mutants with glycerol. (A) Representative Western blots of cell-surface biotinylated protein expression and (B) total biotinylated protein expression without (−) and with (+) addition of 0.5 M glycerol to the medium, as described in Experimental Procedures. Blots were probed with 1:1000 dilution of anti-NaDC1 antibodies, followed by 1:5000 dilution of horseradish peroxidase-linked anti-rabbit antibodies. Chemiluminescent molecular weight markers (Std) are shown in the first lane of each blot.