Table 2.
Steady-State Parameters for rCcP and Fifteen Charge-Reversal Mutants.a
| Major Phase | Minor Phase | |||
|---|---|---|---|---|
| Mutant | KM (μM) | Vmax/e0 (s−1) | KM (μM) | Vmax/e0 (s−1) |
| rCcPb | 2.1 ± 0.2 | 640 ± 20 | ||
| Negative Cluster Mutants | ||||
| R31E | >100 | >870 | 1.2 ± 0.4 | 30 ± 3 |
| E32K | 4.5 ± 0.5 | 750 ± 20 | ||
| D33K | 2.4 ± 0.3 | 680 ± 20 | ||
| D34K | >100 | >450 | 2.7 ± 0.8 | 20 ± 2 |
| E35K | 4.4 ± 0.4 | 760 ± 20 | ||
| D37K | 82 ± 12 | 41 ± 3 | ||
| Mutants Near Ala-193 | ||||
| E201K | 2.9 ± 0.9 | 2.8 ± 0.2 | ||
| E209K | 2.8 ± 0.5 | 200 ± 10 | ||
| D210K | 1.9 ± 0.3 | 470 ± 10 | ||
| Mutants Near Glu-290 | ||||
| E118K | 51 ± 8 | 230 ± 20 | ||
| E290K | 60 ± 11 | 140 ± 10 | ||
| E291K | 3.8 ± 0.4 | 520 ± 10 | ||
| Top Front-Face Mutants | ||||
| E17K | 2.3 ± 0.4 | 420 ± 20 | ||
| D18K | 2.0 ± 0.3 | 380 ± 10 | ||
| E98K | 3.1 ± 0.7 | 690 ± 40 | ||
a
Experimental Conditions: 0.100 M ionic strength, potassium phosphate buffer, pH 7.5, 25 °C, [H2O2] = 200 μM.
b
Data from reference (30).