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. 1985 Mar;53(3):1005–1007. doi: 10.1128/jvi.53.3.1005-1007.1985

Methylation pattern of fish lymphocystis disease virus DNA.

H Wagner, D Simon, E Werner, H Gelderblom, C Darai, R M Flügel
PMCID: PMC254744  PMID: 3973962

Abstract

The content and distribution of 5-methylcytosine in DNA from fish lymphocystis disease virus was analyzed by high-pressure liquid chromatography, nearest-neighbor analysis, and with restriction endonucleases. We found that 22% of all C residues were methylated, including methylation of the following dinucleotide sequences: CpG to 75%, CpC to ca. 1%, and CpA to 2 to 5%. Comparison of relative digestion of viral DNA with MspI and HpaII indicated that CCGG sequences were almost completely methylated at the inner C. The degree of methylation of GCGC was much lower. The methylation pattern of fish lymphocystis disease virus DNA differed from that of the host cell DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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