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. 1985 Mar;53(3):1012–1015. doi: 10.1128/jvi.53.3.1012-1015.1985

The circular intracellular form of Epstein-Barr virus DNA is amplified by the virus-associated DNA polymerase.

J E Shaw
PMCID: PMC254746  PMID: 2983082

Abstract

Selective DNA extraction and hybridization procedures were used to estimate the relative number of covalently closed circular viral genomes in cultures of Epstein-Barr virus (EBV)-transformed cells. In virus-producing P3HR-1 cultures that were exposed for 11 days to phosphonoacetic acid or to acyclovir, the content of covalently closed circular EBV DNA was reduced ca. 70% relative to a control culture without drug. The EBV plasmid content of Raji, a virus nonproducer cell line, was not reduced by exposure to these compounds. When P3HR-1 cultures were exposed to 12-O-tetradecanoylphorbol-13-acetate, the number of circular genomes per cell increased. These findings indicate that two enzyme activities synthesize circular EBV DNA and that the virus-associated DNA polymerase synthesizes most of the circular EBV DNA in a virus producer culture. It is suggested that the circular genomes synthesized by the viral enzyme are intermediates in the syntheses of linear virus DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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