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. 1985 Apr;54(1):236–239. doi: 10.1128/jvi.54.1.236-239.1985

Primer binding sites corresponding to several tRNA species are present in DNAs of different members of the same retrovirus-like gene family (VL30).

A Itin, E Keshet
PMCID: PMC254784  PMID: 3973981

Abstract

We analyzed the putative tRNA primer binding site (PBS) present in several cloned copies of the murine retrovirus-like VL30 family. In the five VL30 DNA clones analyzed, we identified PBS sequences corresponding to three different tRNA species: tRNAPro, tRNAGly, and tRNAGln. The latter two PBS sequences have not been previously encountered in other retroviral or retrovirus-like systems. A unique situation was observed in which PBS sequences complementary to two different tRNA species were flanked by otherwise identical VL30 sequences. In addition, we demonstrated the use of PBS-specific synthetic oligonucleotides for the identification of the tRNA primer and their potential utility in the direct cloning of PBS-containing DNA elements.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Besmer P., Olshevsky U., Baltimore D., Dolberg D., Fan H. Virus-like 30S RNA in mouse cells. J Virol. 1979 Mar;29(3):1168–1176. doi: 10.1128/jvi.29.3.1168-1176.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Devare S. G., Reddy E. P., Law J. D., Aaronson S. A. Nucleotide sequence analysis of the long terminal repeat of integrated simian sarcoma virus: evolutionary relationship with other mammalian retroviral long terminal repeats. J Virol. 1982 Jun;42(3):1108–1113. doi: 10.1128/jvi.42.3.1108-1113.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eibel H., Gafner J., Stotz A., Philippsen P. Characterization of the yeast mobile element Ty1. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):609–617. doi: 10.1101/sqb.1981.045.01.079. [DOI] [PubMed] [Google Scholar]
  4. Hampe A., Gobet M., Even J., Sherr C. J., Galibert F. Nucleotide sequences of feline sarcoma virus long terminal repeats and 5' leaders show extensive homology to those of other mammalian retroviruses. J Virol. 1983 Jan;45(1):466–472. doi: 10.1128/jvi.45.1.466-472.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harada F., Peters G. G., Dahlberg J. E. The primer tRNA for Moloney murine leukemia virus DNA synthesis. Nucleotide sequence and aminoacylation of tRNAPro. J Biol Chem. 1979 Nov 10;254(21):10979–10985. [PubMed] [Google Scholar]
  6. Haseltine W. A., Kleid D. G. A method for classification of 5' termini of retroviruses. Nature. 1978 Jun 1;273(5661):358–364. doi: 10.1038/273358a0. [DOI] [PubMed] [Google Scholar]
  7. Haseltine W. A., Kleid D. G., Panet A., Rothenberg E., Baltimore D. Ordered transcription of RNA tumor virus genomes. J Mol Biol. 1976 Sep 5;106(1):109–131. doi: 10.1016/0022-2836(76)90303-x. [DOI] [PubMed] [Google Scholar]
  8. Hodgson C. P., Elder P. K., Ono T., Foster D. N., Getz M. J. Structure and expression of mouse VL30 genes. Mol Cell Biol. 1983 Dec;3(12):2221–2231. doi: 10.1128/mcb.3.12.2221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Howk R. S., Troxler D. H., Lowy D., Duesberg P. H., Scolnick E. M. Identification of a 30S RNA with properties of a defective type C virus in murine cells. J Virol. 1978 Jan;25(1):115–123. doi: 10.1128/jvi.25.1.115-123.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Itin A., Keshet E. Nucleotide sequence analysis of the long terminal repeat of murine virus-like DNA (VL30) and its adjacent sequences: resemblance to retrovirus proviruses. J Virol. 1983 Sep;47(3):656–659. doi: 10.1128/jvi.47.3.656-659.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Keshet E., Itin A. Patterns of genomic distribution and sequence heterogeneity of a murine "retrovirus-like" multigene family. J Virol. 1982 Jul;43(1):50–58. doi: 10.1128/jvi.43.1.50-58.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Keshet E., Shaul Y., Kaminchik J., Aviv H. Heterogeneity of "virus-like" genes encoding retrovirus-associated 30S RNA and their organization within the mouse genome. Cell. 1980 Jun;20(2):431–439. doi: 10.1016/0092-8674(80)90629-7. [DOI] [PubMed] [Google Scholar]
  13. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  14. Peters G. G., Glover C. Low-molecular-weight RNAs and initiation of RNA-directed DNA synthesis in avian reticuloendotheliosis virus. J Virol. 1980 Feb;33(2):708–716. doi: 10.1128/jvi.33.2.708-716.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sprinzl M., Gauss D. H. Compilation of tRNA sequences. Nucleic Acids Res. 1984;12 (Suppl):r1–57. [PMC free article] [PubMed] [Google Scholar]
  16. Taylor J. M., Illmensee R. Site on the RNA of an avian sarcoma virus at which primer is bound. J Virol. 1975 Sep;16(3):553–558. doi: 10.1128/jvi.16.3.553-558.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Varmus H. E. Form and function of retroviral proviruses. Science. 1982 May 21;216(4548):812–820. doi: 10.1126/science.6177038. [DOI] [PubMed] [Google Scholar]

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