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The Journal of Automatic Chemistry logoLink to The Journal of Automatic Chemistry
. 1993;15(6):227–232. doi: 10.1155/S146392469300029X

Signal-to-noise optimization and evaluation of a home-made visible diode-array spectrophotometer

Ivo M Raimundo, Jr 1, Celio Pasquini 1
PMCID: PMC2548024  PMID: 18924979

Abstract

This paper describes a simple low-cost multichannel visible spectrophotometer built with an RL512G EGG-Reticon photodiode array. A symmetric Czerny-Turner optical design was employed; instrument control was via a single-board microcomputer based on the 8085 Intel microprocessor. Spectral intensity data are stored in the single-board's RAM and then transferred to an IBM-AT 3865X compatible microcomputer through a RS-232C interface. This external microcomputer processes the data to recover transmittance, absorbance or relative intensity of the spectra. The signal-to-noise ratio and dynamic range were improved by using variable integration times, which increase during the same scan; and by the use of either weighted or unweighted sliding average of consecutive diodes. The instrument is suitable for automatic methods requiring quasi-simultaneous multiwavelength detections, such as multivariative calibration and flow-injection gradient scan techniques.

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