Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1985 May;54(2):345–350. doi: 10.1128/jvi.54.2.345-350.1985

The J gene of bacteriophage phi X174: in vitro analysis of J protein function.

R K Hamatake, A Aoyama, M Hayashi
PMCID: PMC254803  PMID: 3157804

Abstract

The J protein of phi X174 is a small, highly basic protein and is a component of the phage capsid. We have investigated the role of J protein during single-stranded viral DNA synthesis and phage morphogenesis by using an in vitro system composed of purified viral and host components (Aoyama et al., Proc. Natl. Acad. Sci. U.S.A. 80:4195-4199, 1983). The characterization of the products made in the presence and absence of J protein shows that J protein is not required for viral DNA synthesis, but is required for the packaging of DNA into infectious phage. The ability of J protein to bind to double-stranded DNA as well as single-stranded DNA and other interactions with DNA suggest a model in which J protein binds to double-stranded, replicative form DNA and enters the phage prohead by remaining bound to viral DNA as it is encapsidated.

Full text

PDF
345

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts B. M., Frey L. T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA. Nature. 1970 Sep 26;227(5265):1313–1318. doi: 10.1038/2271313a0. [DOI] [PubMed] [Google Scholar]
  2. Aoyama A., Hamatake R. K., Hayashi M. In vitro synthesis of bacteriophage phi X174 by purified components. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4195–4199. doi: 10.1073/pnas.80.14.4195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aoyama A., Hamatake R. K., Hayashi M. Morphogenesis of phi X174: in vitro synthesis of infectious phage from purified viral components. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7285–7289. doi: 10.1073/pnas.78.12.7285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Arai K., Low R., Kobori J., Shlomai J., Kornberg A. Mechanism of dnaB protein action. V. Association of dnaB protein, protein n', and other repriming proteins in the primosome of DNA replication. J Biol Chem. 1981 May 25;256(10):5273–5280. [PubMed] [Google Scholar]
  5. Burgess A. B. Studies on the proteins of phi X174. II. The protein composition of the phi X coat. Proc Natl Acad Sci U S A. 1969 Oct;64(2):613–617. doi: 10.1073/pnas.64.2.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Earnshaw W. C., Casjens S. R. DNA packaging by the double-stranded DNA bacteriophages. Cell. 1980 Sep;21(2):319–331. doi: 10.1016/0092-8674(80)90468-7. [DOI] [PubMed] [Google Scholar]
  7. Eisenberg S., Griffith J., Kornberg A. phiX174 cistron A protein is a multifunctional enzyme in DNA replication. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3198–3202. doi: 10.1073/pnas.74.8.3198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eisenberg S., Scott J. F., Kornberg A. Enzymatic replication of viral and complementary strands of duplex DNA of phage phiX174 proceeds by seprate mechanisms. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3151–3155. doi: 10.1073/pnas.73.9.3151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Freymeyer D. K., 2nd, Shank P. R., Edgell M. H., Hutchison C. A., 3rd, Vanaman T. C. Amino acid sequence of the small core protein from bacteriophage phiX174. Biochemistry. 1977 Oct 18;16(21):4550–4556. doi: 10.1021/bi00640a002. [DOI] [PubMed] [Google Scholar]
  10. Fujisawa H., Hayashi M. Assembly of bacteriophage phi X174: identification of a virion capsid precursor and proposal of a model for the functions of bacteriophage gene products during morphogenesis. J Virol. 1977 Oct;24(1):303–313. doi: 10.1128/jvi.24.1.303-313.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fujisawa H., Hayashi M. Functions of gene C and gene D products of bacteriophage phi X 174. J Virol. 1977 Feb;21(2):506–515. doi: 10.1128/jvi.21.2.506-515.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fujisawa H., Hayashi M. Viral DNA-synthesizing intermediate complex isolated during assembly of bacteriophage phi X174. J Virol. 1976 Aug;19(2):409–415. doi: 10.1128/jvi.19.2.409-415.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gosule L. C., Schellman J. A. Compact form of DNA induced by spermidine. Nature. 1976 Jan 29;259(5541):333–335. doi: 10.1038/259333a0. [DOI] [PubMed] [Google Scholar]
  14. Kodaira K., Taketo A. Isolation and some properties of bacteriophage alpha3 gene J mutant. Mol Gen Genet. 1984;195(3):541–543. doi: 10.1007/BF00341460. [DOI] [PubMed] [Google Scholar]
  15. Kornberg A., Scott J. F., Bertsch L. L. ATP utilization by rep protein in the catalytic separation of DNA strands at a replicating fork. J Biol Chem. 1978 May 10;253(9):3298–3304. [PubMed] [Google Scholar]
  16. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  17. Mukai R., Hamatake R. K., Hayashi M. Isolation and identification of bacteriophage phi X174 prohead. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4877–4881. doi: 10.1073/pnas.76.10.4877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mukai R., Hayashi M. In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174. J Virol. 1977 Jun;22(3):619–625. doi: 10.1128/jvi.22.3.619-625.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  20. Sanger F., Coulson A. R., Friedmann T., Air G. M., Barrell B. G., Brown N. L., Fiddes J. C., Hutchison C. A., 3rd, Slocombe P. M., Smith M. The nucleotide sequence of bacteriophage phiX174. J Mol Biol. 1978 Oct 25;125(2):225–246. doi: 10.1016/0022-2836(78)90346-7. [DOI] [PubMed] [Google Scholar]
  21. Scott J. F., Eisenberg S., Bertsch L. L., Kornberg A. A mechanism of duplex DNA replication revealed by enzymatic studies of phage phi X174: catalytic strand separation in advance of replication. Proc Natl Acad Sci U S A. 1977 Jan;74(1):193–197. doi: 10.1073/pnas.74.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shank P. R., Hutchinson C. A., 3rd, Edgell M. H. Isolation and characterization of the four major proteins in the virion of bacteriophage phiX174. Biochemistry. 1977 Oct 18;16(21):4545–4549. doi: 10.1021/bi00640a001. [DOI] [PubMed] [Google Scholar]
  23. Shlomai J., Kornberg A. Deoxyuridine triphosphatase of Escherichia coli. Purification, properties, and use as a reagent to reduce uracil incorporation into DNA. J Biol Chem. 1978 May 10;253(9):3305–3312. [PubMed] [Google Scholar]
  24. Siegel J. E., Hayashi M. Phi-X-174 bacteriophage structural mutants which affect deoxyribonucleic acid synthesis. J Virol. 1969 Oct;4(4):400–407. doi: 10.1128/jvi.4.4.400-407.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weiner J. H., Bertsch L. L., Kornberg A. The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication. J Biol Chem. 1975 Mar 25;250(6):1972–1980. [PubMed] [Google Scholar]
  26. Wilson R. W., Bloomfield V. A. Counterion-induced condesation of deoxyribonucleic acid. a light-scattering study. Biochemistry. 1979 May 29;18(11):2192–2196. doi: 10.1021/bi00578a009. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES