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. 1985 Jun;54(3):899–901. doi: 10.1128/jvi.54.3.899-901.1985

Role of the 3' long open reading frame region of bovine leukemia virus in the maintenance of cell transformation.

R Kettmann, Y Cleuter, D Gregoire, A Burny
PMCID: PMC254885  PMID: 2987544

Abstract

Viral RNA expression was studied by dot blot hybridization with polyadenylated RNAs extracted from a bovine (YR-1) and an ovine (YR-2) tumor cell clone. Both clones were derived from in vivo bovine leukemia virus-induced tumors. The probes used were either the bovine leukemia virus information or only the long open reading frame sequences. No viral RNA corresponding to the bovine leukemia virus long open reading frame region was detected in YR-2, and a very limited amount of bovine leukemia virus messages was unraveled in YR-1. These results strongly suggest that viral expression, even in the long open reading frame region, is not required to maintain transformation of at least some tumor cells.

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Selected References

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  1. Baliga V., Ferrer J. F. Expression of the bovine leukemia virus and its internal antigen in blood lymphocytes. Proc Soc Exp Biol Med. 1977 Nov;156(2):388–391. doi: 10.3181/00379727-156-39942. [DOI] [PubMed] [Google Scholar]
  2. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  3. Deschamps J., Kettmann R., Burny A. Experiments with cloned complete tumor-derived bovine leukemia virus information prove that the virus is totally exogenous to its target animal species. J Virol. 1981 Nov;40(2):605–609. doi: 10.1128/jvi.40.2.605-609.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Franchini G., Wong-Staal F., Gallo R. C. Human T-cell leukemia virus (HTLV-I) transcripts in fresh and cultured cells of patients with adult T-cell leukemia. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6207–6211. doi: 10.1073/pnas.81.19.6207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grégoire D., Couez D., Deschamps J., Heuertz S., Hors-Cayla M. C., Szpirer J., Szpirer C., Burny A., Huez G., Kettmann R. Different bovine leukemia virus-induced tumors harbor the provirus in different chromosomes. J Virol. 1984 Apr;50(1):275–279. doi: 10.1128/jvi.50.1.275-279.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gupta P., Ferrer J. F. Expression of bovine leukemia virus genome is blocked by a nonimmunoglobulin protein in plasma from infected cattle. Science. 1982 Jan 22;215(4531):405–407. doi: 10.1126/science.6276975. [DOI] [PubMed] [Google Scholar]
  7. Hahn B., Manzari V., Colombini S., Franchini G., Gallo R. C., Wong-Staal F. Common site of integration of HTLV in cells of three patients with mature T-cell leukaemia-lymphoma: a retraction. Nature. 1983 Sep 22;305(5932):340–340. doi: 10.1038/305340a0. [DOI] [PubMed] [Google Scholar]
  8. Kettmann R., Deschamps J., Cleuter Y., Couez D., Burny A., Marbaix G. Leukemogenesis by bovine leukemia virus: proviral DNA integration and lack of RNA expression of viral long terminal repeat and 3' proximate cellular sequences. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2465–2469. doi: 10.1073/pnas.79.8.2465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kettmann R., Deschamps J., Couez D., Claustriaux J. J., Palm R., Burny A. Chromosome integration domain for bovine leukemia provirus in tumors. J Virol. 1983 Jul;47(1):146–150. doi: 10.1128/jvi.47.1.146-150.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kettmann R., Marbaix G., Cleuter Y., Portetelle D., Mammerickx M., Burny A. Genomic integration of bovine leukemia provirus and lack of viral RNA expression in the target cells of cattle with different responses to BLV infection. Leuk Res. 1980;4(6):509–519. doi: 10.1016/0145-2126(80)90062-4. [DOI] [PubMed] [Google Scholar]
  11. Marbaix G., Kettmann R., Cleuter Y., Burny A. Viral RNA content of bovine leukemia virus-infected cells. Mol Biol Rep. 1981 May 22;7(1-3):135–138. doi: 10.1007/BF00778744. [DOI] [PubMed] [Google Scholar]
  12. Portetelle D., Bruck C., Burny A., Dekegel D., Mammerickx M., Urbain J. Detection of complement-dependent lytic antibodies in sera from bovine leukemia virus-infected animals. Ann Rech Vet. 1978;9(4):667–674. [PubMed] [Google Scholar]
  13. Rice N. R., Stephens R. M., Couez D., Deschamps J., Kettmann R., Burny A., Gilden R. V. The nucleotide sequence of the env gene and post-env region of bovine leukemia virus. Virology. 1984 Oct 15;138(1):82–93. doi: 10.1016/0042-6822(84)90149-1. [DOI] [PubMed] [Google Scholar]
  14. Rosen C. A., Sodroski J. G., Kettman R., Burny A., Haseltine W. A. Trans activation of the bovine leukemia virus long terminal repeat in BLV-infected cells. Science. 1985 Jan 18;227(4684):320–322. doi: 10.1126/science.2981432. [DOI] [PubMed] [Google Scholar]
  15. Seiki M., Eddy R., Shows T. B., Yoshida M. Nonspecific integration of the HTLV provirus genome into adult T-cell leukaemia cells. Nature. 1984 Jun 14;309(5969):640–642. doi: 10.1038/309640a0. [DOI] [PubMed] [Google Scholar]
  16. Seiki M., Hattori S., Hirayama Y., Yoshida M. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618–3622. doi: 10.1073/pnas.80.12.3618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sodroski J. G., Rosen C. A., Haseltine W. A. Trans-acting transcriptional activation of the long terminal repeat of human T lymphotropic viruses in infected cells. Science. 1984 Jul 27;225(4660):381–385. doi: 10.1126/science.6330891. [DOI] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Westin E. H., Wong-Staal F., Gelmann E. P., Dalla-Favera R., Papas T. S., Lautenberger J. A., Eva A., Reddy E. P., Tronick S. R., Aaronson S. A. Expression of cellular homologues of retroviral onc genes in human hematopoietic cells. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2490–2494. doi: 10.1073/pnas.79.8.2490. [DOI] [PMC free article] [PubMed] [Google Scholar]

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