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. 1985 Jul;55(1):96–100. doi: 10.1128/jvi.55.1.96-100.1985

Monkey B-lymphotropic papovavirus mutant capable of replicating in T-lymphoblastoid cells.

T Kanda, K K Takemoto
PMCID: PMC254902  PMID: 3925162

Abstract

Monkey B-lymphotropic papovavirus (LPV) DNA present as free copies in LPV-transformed hamster embryo cells was molecularly cloned in Escherichia coli. Twenty-two of 24 cloned DNAs were 4.9 kilobases long and shorter than the wild-type LPV DNA (5.1 kilobases). The shorter DNA was nondefective and generated infectious virus (designated LPV-76) upon transfection of human B-lymphoblastoid BJA-B cells. LPV-76 DNA had a small deletion in the early region and a deletion and an insertion in the control region for transcription. LPV-76 VP-1 was apparently larger than that of the wild-type LPV. LPV-76 could grow in human T-lymphoblastoid MOLT-4 cells, whereas the wild-type LPV replicated only in B-lymphoblastoid cells. Characterization of constructed recombinant viruses between wild-type LPV and LPV-76 showed that the mutation responsible for the extended host range of LPV-76 was within the PstI B fragment, which includes the VP-1 coding region. These data strongly suggest that the mutation of VP-1 altered the host range of LPV.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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